Journal of Dental Rehabilitation and Applied Science (구강회복응용과학지)

Korean Academy of Stomatognathic Function and Occlusion (대한턱관절교합학회)
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Effects of Shiitake mushroom extract on antimicrobial activity against periodontopathogens and inflammatory condition of human gingival fibroblast

치주 질환관련 세균의 항균 및 세포 염증에 대한 표고버섯 추출물의 효과

  • Jeon, Yeol-Mae (Department of Preventive Dentistry, College of Dentistry, Dankook University)
  • 전열매 (단국대학교 치과대학 예방치과학교실)
  • Received : 2022.05.28
  • Accepted : 2022.06.14
  • Published : 2022.06.30
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Abstract

Purpose: The purpose of this study was to investigate antimicrobial activity of extracts from shiitake mushroom against periodontopathogens and its cytotoxicity for human gingival fibroblast. Materials and Methods: Shiitake mushroom was soaked in water and acetone, and the supernatant was dried to collect its extract. The susceptibility of periodontopathogens for the extracts was investigated. Human gingival fibroblast was treated with the extracts, and the cell viability was measured CCK-8 solution. Results: The water extract from shiitake mushroom significantly reduced the growth of periodontopathogens at 2.5 mg/ml (P < 0.05). The acetone extract significantly inhibited the growth of Porphyromonas gingivalis and Tannerella forsythia at 0.32 mg/ml and Treponema denticola growth at 0.64 mg/ml (P < 0.05). The cytotoxicity of the extract was shown at a concentration of 2.5 mg/ml. The extracts with a concentration of 1.25 mg/ml appeared to be reduce cell viability after 4 h. Conclusion: The extracts of shiitake mushroom have antimicrobial activity against periodontitis-causing bacteria and relieving inflammation. Therefore, the extracts may be a candidate for preventing and treating periodontal disease.

목적: 이 연구의 목적은 표고 버섯 추출물의 치주 병원체에 대한 항균 활성과 인간 치은 섬유아세포에 대한 세포 독성을 조사하는 것입니다. 연구 재료 및 방법: 표고버섯을 물과 아세톤에 닮근 후, 상등액을 건조하여 추출물을 채취하였다. 추출물에 대한 치주 병원체의 감수성을 조사하였다. 인간 치은 섬유아세포에 추출물을 처리하고 CCK-8 용액을 이용하여 세포 생존율을 측정하였다. 결과: 표고버섯 물추출물은 2.5 mg/ml에서 치주병원체의 증식을 유의하게 감소시켰다(P < 0.05). 아세톤 추출물은 0.32 mg/ml에서 Porphyromonas gingivalis와 Tannerella forsythia의 성장을 유의하게 억제하였고, 0.64 mg/ml에서 Treponema denticola의 성장을 유의하게 억제하였다(P < 0.05). 추출물의 세포독성은 2.5 mg/ml의 농도에서 나타났으며, 1.25 mg/ml 농도의 추출물이 4시간 후부터 세포 생존률을 감소시키는 것으로 나타났다. 결론: 표고버섯 추출물은 치주염 유발 세균에 대한 항균 활성과 염증 완화 효과를 보였다. 따라서 추출물은 치주질환 예방 및 치료에 대한 후보물질이 될 수 있다.

Keywords

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