Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
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Hesperidin and Hesperetin Protect against Oxidative Stress on Hepatic Toxicity in Rats

Hesperidin과 Hesperetin의 간 손상 동물모델에서 산화적 스트레스에 대한 간 보호 효과

  • Kim, Ji Hyun (Department of Food Science and Nutrition, Gyeongsang National University) ;
  • Li, Li (Department of Food Science and Nutrition, Pusan National University) ;
  • Kim, Mi Suk (Department of Food Science, Gyeongsang National University) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University) ;
  • Kim, Hyun Young (Department of Food Science and Nutrition, Gyeongsang National University) ;
  • Choi, Jine Shang (Department of Food Science and Technology, Gyeongsang National University)
  • 김지현 (경상국립대학교 식품영양학과) ;
  • 이여 (부산대학교 식품영양학과) ;
  • 김미숙 (경상국립대학교 식품과학과) ;
  • 조은주 (부산대학교 식품영양학과) ;
  • 김현영 (경상국립대학교 식품영양학과) ;
  • 최진상 (경상국립대학교 식품공학과)
  • Received : 2022.04.26
  • Accepted : 2022.06.08
  • Published : 2022.06.30
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Abstract

Objectives: To investigate the protective effect of hesperidin and hesperetin against oxidative stress in 2,2'-azobis (2-aminopropane) dihydrochloride (AAPH)-induced liver toxicity in rats. Methods: Hesperidin or hesperetin (200 mg/kg/day, respectively) was orally administered for 7 days once daily in rats. Subsequently, AAPH (50 mg/kg/day) was administered intraperitoneally. Lipid peroxidation, nitric oxide production, catalase activity, and protein expressions of nuclear factor-kappa B (NF-κB) and inducible nitric oxide synthase (iNOS) in the liver tissues were measured. Results: Administration of hesperidin and hesperetin significantly decreased serum aspartate transaminase and alanine transaminase levels in AAPH-induced oxidative stress liver tissues compared with control group. Lipid peroxidation and nitric oxide (NO) production were also significantly reduced by hesperidin and hesperetin in AAPH-induced oxidative stress liver tissues. In particular, lipid peroxidation levels of hesperetin-administered group significantly decreased to 5.02 nmole/mg protein in oxidative stress rats. Hesperidin and hesperetin significantly increased antioxidant activity, such as that of catalase. Furthermore, administration of hesperidin and hesperetin substantially down-regulated the expression of NF-κB and iNOS in liver tissues. Administration of hesperidin reduced NO levels and iNOS expression more than in the hesperetin-administered group. Conclusions: Administration of hesperidin and hesperetin led to a reduction in AAPH-induced liver toxicity by regulating oxidative stress.

Keywords

References

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