Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Analysis of enzyme activity changes caused by flooding stress in upland crops

침수 스트레스에 의한 밭작물의 효소활성 변화 분석

  • Juhyung Shin (Department of Integrative Biology, Kyungpook National University) ;
  • Byeonggyu Kim (Department of Integrative Biology, Kyungpook National University) ;
  • Kihwan Kim (Department of Applied Biosciences, Kyungpook National University) ;
  • Tae-An Kang (Department of Applied Biosciences, Kyungpook National University) ;
  • Won-Chan Kim (Department of Integrative Biology, Kyungpook National University)
  • 신주형 (경북대학교 농생명융합공학과) ;
  • 김병규 (경북대학교 농생명융합공학과) ;
  • 김기환 (경북대학교 응용생명과학과) ;
  • 강태안 (경북대학교 응용생명과학과) ;
  • 김원찬 (경북대학교 농생명융합공학과)
  • Received : 2022.08.19
  • Accepted : 2022.09.20
  • Published : 2022.09.30
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Abstract

Among various environmental stresses, humid stress lacks mechanisms and biochemical understanding compared to drought, low temperature, and high salt stresses. The aim of this study was to investigate enzyme activity of field crops under humidity stress. Results of this study could be used as basic data for understanding humidity stress and early diagnosis. Growth and enzyme activities of sesame, perilla, red beans, sorghum, and beans as major field crops in Korea when flooded were investigated. It was confirmed that growths of both shoots and roots were retarded. In plants, anaerobic fermentation occurred due to flooding stress, which increased the activity of alcohol dehydrogenase (ADH) compared to the control group. Increases of reactive oxygen species (ROS) were also observed. All flooded plants showed increased peroxidase (POD) activity and lipid peroxidation. Their dyeing strength was darker than that of the control group, even in 3,3'-diaminobenzidine (DAB) staining. Since enzyme activity changes in plants appear relatively faster than changes in phenotype at the ground level, they could be used as biomarkers for early diagnosis of humidity stress in crops.

다양한 환경 스트레스 중 습해 스트레스의 경우 기타 가뭄이나 저온, 고염 등에 비하여 메커니즘과 생화학적 이해가 부족한 실정이다. 본 연구는 습해 스트레스 하에서의 밭작물의 효소활성에 대하여 조사하여 습해 스트레스에 대한 이해와 조기 진단을 위한 기초자료로 활용하고자 수행하였다. 국내의 주요 밭작물인 참깨, 들깨, 팥, 수수, 콩 등을 대상으로 침수 스트레스를 주었을 시 생육도와 식물 내부의 변화에 대하여 조사하였는데, 스트레스를 받을 경우 지하부는 물론 지상부에서도 생육의 저하가 일어나는 것을 확인하였다. 식물체 내에서는 침수 스트레스에 의하여 혐기적 발효가 일어나 대조군에 비하여 ADH의 활성이 높아지는 것을 확인하였으며 스트레스를 받을 시 발생하는 ROS에 의한 변화 또한 확인하였다. 습해를 받은 식물들 모두 POD 활성과 lipid peroxidation이 증가하였고 이를 시각화한 DAB 염색에서도 염색 강도가 대조군에 비하여 진하게 염색되었다. 지상부에서 표현형의 변화보다 식물 체내의 효소활성의 변화가 비교적 빠르게 나타나므로 추후 작물들의 습해 스트레스 조기진단을 위한 생체지표로써 활용될 수 있을 것으로 사료된다.

Keywords

Acknowledgement

본 논문은 농촌진흥청 공동연구사업(과제번호: PJ015683022021)의 지원에 의해 이루어졌다.

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