한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

Ulva grossa sp. nov. (Ulvales, Chlorophyta) from Korea based on Molecular and Morphological Analyses

  • Kang, Pil Joon (Department of Marine Biology, Pukyong National University) ;
  • An, Jae Woo (Department of Marine Biology, Pukyong National University) ;
  • Nam, Ki Wan (Department of Marine Biology, Pukyong National University)
  • 투고 : 2022.04.21
  • 심사 : 2022.06.08
  • 발행 : 2022.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A green alga specimen was collected from the eastern coast of Korea. This species shared the typical features of genus Ulva and was characterized by irregularly shaped thalli, relatively small and thick thallus, entire undulate margins without serrations, and one or two pyrenoids per cell. In a phylogenetic tree, based on sequences of the nuclear-encoded internal transcribed spacer region, it nests as a sister clade to a few species including Ulva ohnoi, which has a relatively large thallus. This Korean algal specimen differs from the species forming the same subclades, including U. ohnoi, Ulva fasciata, Ulva reticulata, and Ulva gigantean, and has a relatively small (3-8 cm) and thick (60-100 ㎛) thallus. Of these species, U. ohnoi, originally described from Japan, is similar to the Korean alga as it had a thick thallus of 30-90 ㎛, but it has microscopic serrations on the thallus margin, unlike the Korean alga. The genetic distance between the Korean alga species and the aforementioned species was determined to be 1.8%-4.8%, indicating an inter-specific divergence level at the genus Ulva. Herein, Ulva grossa sp. nov. (Ulvales, Chlorophyta) from Korea is described based on the morphological and molecular analyses.

키워드

과제정보

This work was supported by a Research Grant of Pukyong National University(2021)

참고문헌

  1. Kazi, M.A., Kavale, M.G., Singh, V.V. 2016. Morphological and molecular characterization of Ulva chaugulii sp. nov., U. lactuca and U. ohnoi (Ulvophyceae, Chlorophyta) from India. Phycologia 55, 45-54. https://doi.org/10.2216/15-11.1
  2. Guiry, M.D. in Guiry, M.D., Guiry, G.M. 2022. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. Available from http://www.algaebase.org. Accessed: 25 April 2022.
  3. Fletcher, R.L. 1996. The occurrence of 'green tides' - a review. In: Schramm W, Nienhuis PH (eds) Marine benthic vegetation in Europe: recent changes and the effect of eutrophication. Springer, Heidelberg, pp 7-43.
  4. Blomster, J., Back, S., Fewer, D.P., Kiirikki, M., Lehvo, A., Maggs, C.A., Stanhope, M.J. 2002. Novel morphology in Enteromorpha (Ulvophyceae) forming green tides. Am. J. Bot. 89, 1756-1763. https://doi.org/10.3732/ajb.89.11.1756
  5. Zhao, J., Jiang, P., Liu, Z., Wang, J., Cui, Y., Qin, S. 2010. Genetic variation of Ulva (Enteromorpha) prolifera (Ulvales, Chlorophyta) - the causative species of the green tides in the Yellow Sea, China. J. Appl. Phycol. 23, 227-233. https://doi.org/10.1007/s10811-010-9563-1
  6. Bliding, C. 1968. A critical survey of European taxa in Ulvales. II. Ulva, Ulvaria, Monostroma, Kornmannia. Bot. Notiser. 121,535-629.
  7. Koeman, R.P.T., van den Hoek, C. 1981. The taxonomy of Ulva (Chlorophyceae) in the Netherlands. Br. Phycol. J. 16, 9-53. https://doi.org/10.1080/00071618100650031
  8. Blomster, J., Maggs, C.A., Stanhope, M.J. 1998. Molecular and morphological analysis of Enteromorpha intestinalis and E. compressa (Chlorophyta) in the British Isles. J. Phycol. 34, 319-340. https://doi.org/10.1046/j.1529-8817.1998.340319.x
  9. Hayden, H.S., Blomster, J., Maggs, C.A., Silva, P.C., Stanhope, M.J., Waaland, J.R. 2003. Linnaeus was right all along: Ulva and Enteromorpha are not distinct genera. Eur. J. Phycol. 38, 277-294. https://doi.org/10.1080/1364253031000136321
  10. Shimada, S., Hiraoka, M., Nabata, S., lima, M., Masuda, M. 2003. Molecular Phylogenetic analyses of the Japanese Ulva and Enteromorpha (Ulvales, Ulvophyceae), with special reference to the free-floating Ulva. Phycol. Res. 51, 99-108. https://doi.org/10.1046/j.1440-1835.2003.00296.x
  11. O'Kelly, C.J., Kurihara, A., Shipley, T.C., Sherweed, A.R. 2010. Molecular assessment of Ulva spp. (Ulvophyceae, Chlorophyta) in the Hawaiian Islands. J. Phycol. 46, 728-735. https://doi.org/10.1111/j.1529-8817.2010.00860.x
  12. Liu, F., Pang, S.J., Xu, N., Shan, T.F., Sun, S., Hu, X., Yang, J.Q. 2010. Ulva diversity in the Yellow Sea during the large-scale green algal blooms in 2008-2009. Phycol. Res. 58, 270-279. https://doi.org/10.1111/j.1440-1835.2010.00586.x
  13. Guidone, M., Thornber, C., Wysor, B., O'Kelly, C.J. 2013. Molecular and morphological diversity of Narragansett Bay (RI, USA) Ulva (Ulvales, Chlorophyta) populations. J. Phycol. 49, 979-995 https://doi.org/10.1111/jpy.12108
  14. Guoying, D., Feifei, W., Yunxiang, M., Shenghua, G., Hongfan, X., Guiq, B. 2014. DNA barcoding assessment of green macroalga in coastal zone around Qingdao, China. J. Ocean. Univ. China. 13, 97-103. https://doi.org/10.1007/s11802-014-2197-1
  15. Heesch, S., Broom, J.E.S., Neill, K.F., Farr, T.J., Dalen, J.L., Nelson, W.A. 2009. Ulva, UmbraUlva and Gemina: genetic survey of New Zealand taxa reveals diversity and introduced species. Eur. J. Phycol. 44, 143-154. https://doi.org/10.1080/09670260802422477
  16. Hofmann, L.C., Nettleton, J.C., Neefus, C.D., Mathieson, A.C. 2010. Cryptic diversity of Ulva (Ulvales, Chlorophyta) in the Great Bay Estuarine System (Atlantic USA): introduced and indigenous distromatic species. Eur. J. Phycol. 45, 230-239. https://doi.org/10.1080/09670261003746201
  17. Kraft, L.G.K., Kraft, G.T, Waller, R.F. 2010. Investigations into southern Australia Ulva (Ulvophyceae, Chlorophyta) taxonomy and molecular phylogeny indicate both cosmopolitanism and endemic cryptic species. J. Phycol. 46, 1257-1277. https://doi.org/10.1111/j.1529-8817.2010.00909.x
  18. Wolf, M.A., Sciuto, K., Androli, C., Moro, I. 2012. Ulva (Chlorophyta, Ulvales) biodiversity in the North Adriatic Sea (Mediterranean, Italy): cryptic species and new introductions. J. Phycol. 48, 1510-1521. https://doi.org/10.1111/jpy.12005
  19. Kirkendale, L., Saunders, G.W., Winberg, P. 2013. A molecular survey of Ulva (Chlorophyta) in temperate Australia reveals enhanced levels of cosmopolitanism. J. Phycol. 49: 69-81. https://doi.org/10.1111/jpy.12016
  20. Loughnane, C.J., McIvor, L.M., Rindi, F., Stengel, D.B., Guiry, M.D. 2008. Morphology, rbcL phylogeny and distribution of distromatic Ulva (Ulvophyceae, Chlorophyta) in Ireland and southern Britain. Phycologia 47, 416-429. https://doi.org/10.2216/PH07-61.1
  21. Matsumono, K., Shimada, S. 2015. Systematics of green algae resembling Ulva conglobata, with a description of Ulva adhaerens sp. nov. (Ulvales, Ulvophyceae). Eur. J. Phycol. 50, 100-111. https://doi.org/10.1080/09670262.2014.994189
  22. Lee, I.K., Kang, J.W. 1986. A check list of marine algae in Korea. Kor. J. Phycol. 1, 311-325.
  23. Lee, Y.P., Kang, S.Y. 2002. A Catalogue of the Seaweeds in Korea. Jeju National University Press, Jeju, Korea. 662 pp.
  24. Lee, Y.P. 2008. Marine Algae of Jeju. Academy Publishing Co, Seoul, Korea. 477 pp.
  25. Bae, E.H. 2010. Ulotrichales, Ulvales. In: Bae EH, Kim HS, Kwon CJ, Hwang IK, Kim GH, Klochkova TA (eds) Algal Flora of Korea. Volume 1, Number 1. Chlorophyta: Ulvophyceae: Ulotrichales, Ulvales, Cladophorales, Bryopsidales. Marine Green Algae. National Institute of Biological Resources, Incheon, pp 7-52.
  26. Kim, H.S., Boo, S.M., Lee, I.K., Sohn, C.H. 2013. National List of Species of Korea: Marine Algae. Jeonghaengsa, Seoul, Korea. 336 pp.
  27. An, J.W. and Nam, K.W. 2017. First record of Ulva torta (Ulvales, Chlorophyta) in Korea. Korean. J. Environ. Biol. 35, 329-334. https://doi.org/10.11626/KJEB.2017.35.3.329
  28. Bliding, C. 1963. A critical survey of European taxa in Ulvales. Part I. Capsosiphon, Percursaria, Blidingia, Enteromorpha. Op. Bot. Soc. Bot. Lund. 8, 1-160.
  29. Harper, J.T., Saunders, G.W. 2001. Molecular systematics of the Florideophyceae (Rhodophyta) using nuclear large and small subunit rDNA sequence data. J. Phycol. 37, 1073-1082. https://doi.org/10.1046/j.1529-8817.2001.00160.x
  30. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic. Acids. Symp. Ser. 41, 95-98.
  31. Tamura, K. 1992. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Mol. Biol. Evol. 9, 678-687.
  32. Linnaeus, C. 1753. Species plantarum, exhibentes plantas rite cognitas, ad genera relatas, cum differentiis specificis, nominibus trivialibus, synonymis selectis, locis natalibus, secundum systema sexuale digestas. Vol. 1. Impensis Laurentii Salvii, Holmiae [Stockholm], Sweden. 560 pp.
  33. Steffensen, D.A. 1976. The effect of nutrient enrichment and temperature on the growth in culture of Ulva lactuca L. Aqua. Bot. 2, 337-351. https://doi.org/10.1016/0304-3770(76)90031-0
  34. Mshigeni, K.E., Kajumulo, A.A. 1979. Effects of the environment on polymorphism in Ulva fasciata Delile (Chlorophyta, Ulvaceae). Bot. Mar. 22, 145-148.
  35. Tanner, C.E. 1986. Investigations of the taxonomy and morphological variation of Ulva (Chlorophyta): Ulva californica Wille. Phycologia 25, 510-520. https://doi.org/10.2216/i0031-8884-25-4-510.1
  36. Phillips, J.A. 1988. Field, anatomical and developmental studies on southern Australian species of Ulva (Ulvaceae, Chlorophyta). Aust. Syst. Bot. 1, 411-456. https://doi.org/10.1071/SB9880411
  37. Malta, E.J., Draisma, S.G.A., Kamermans, P. 1999. Free-floating Ulva in the southwest Netherlands: species or morphotypes? A morphological, molecular and ecological comparison. Eur. J. Phycol. 34, 443-454. https://doi.org/10.1080/09541449910001718801
  38. Hiraoka, M., Shimada, S., Uenosono, M., Masuda, M. 2003. A new green-tide-forming alga, Ulva ohnoi Hiraoka et Shimada sp. nov. (Ulvales, Ulvophyceae) from Japan. Phycol. Res. 51, 14-29.
  39. Womersley, H.B.S. 1984. The Marine Benthic Flora of Southern Australia. Part I. Government Printer, Adelaide, Australia. 329 pp.
  40. Dawes, C.J., Mathieson, A.C. 2008. The seaweeds of Florida. University Press of Florida, Gainesville, Florida, U.S.A. 591 pp.
  41. Silva, P.C., Basson, P.W., Moe, R.L. 1996. Catalogue of the benthic marine algae of the Indian Ocean. Univ. Calif. Publ. in. Bot. 79, 1-1259.
  42. Hayden, H.S., Waaland, J.R. 2002. Phylogenetic systematics of the Ulvaceae (Ulvales, Ulvophyceae) using chloroplast and nuclear DNA sequences. J. Phycol. 38, 1200-1212. https://doi.org/10.1046/j.1529-8817.2002.01167.x
  43. Ichihara, K., Arai, S., Uchimura, M., Fay, E.J., Ebata, H., Hiraoka, M., Shimada, S. 2009. New species of freshwater Ulva, Ulva limnetica (Ulvales, Ulvophyceae) from the Ryukyu Islands, Japan. Phycol. Res. 57, 94-103. https://doi.org/10.1111/j.1440-1835.2009.00525.x
  44. Abbott, I.A. and Huisman, J.M. 2004. Marine green and brown algae of the Hawaiian Islands. Bishop Museum Press, Honolulu, U.S.A. 259 pp.
  45. Brodie, J., Maggs, C.A., John, D.M. 2007. Green Seaweeds of Britain and Ireland. British Phycological Society, London, UK. 242 pp.