한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제35권3호
  • /
  • Pages.178-184
  • /
  • 2022
  • /
  • 1225-4339(pISSN)
  • /
  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

[10]-Gingerol Induces Intrinsic Apoptosis in A2058 Human Melanoma Cells

  • Guon, Tae Eun (Dept. of Food and Nutrition, Duksung Women's University) ;
  • Chung, Ha Sook (Dept. of Food and Nutrition, Duksung Women's University)
  • 투고 : 2022.04.08
  • 심사 : 2022.05.10
  • 발행 : 2022.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The objective of the present study was to investigate the molecular mechanisms involved in the activity of [10]-gingerol using A2058 human melanoma cells. [10]-Gingerol inhibited the proliferation of A2058 cells by 50% at a concentration of 52 μM. Such inhibition was dose-dependent accompanied by morphological change indicative of apoptosis. Furthermore, flow cytometric analysis by Annexin V and PI double staining showed that [10]-gingerol increased the extent of apoptosis. Analysis of the mechanism of these events indicated that [10]-gingerol increased the ratio of Bax to Bcl-2, resulting in the activation of caspase-9, caspase-3, and poly-ADP-ribose polymerase in a dose-dependent manner.

키워드

참고문헌

  1. Adams JM, Cory S. 1998. The Bcl-2 protein family: Arbiters of cell survival. Science 281:1322-1326 https://doi.org/10.1126/science.281.5381.1322
  2. Araya LE, Soni IV, Hardy JA, Julien O. 2021. Deorphanizing caspase-3 and caspase-9 substrates in and out of apoptosis with deep substrate profiling. ACS Chem Biol 16:2280-2296 https://doi.org/10.1021/acschembio.1c00456
  3. Armstrong JS. 2007. Mitochondrial medicine: Pharmacological targeting of mitochondria in disease. Br J Pharmacol 151:1154-1165 https://doi.org/10.1038/sj.bjp.0707288
  4. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res 47:936-942
  5. Chen J, He X, Peng H, Ou-Yang X, He X. 2008. Research on the antitumor effect of ginsenoside Rg3 in B16 melanoma cells. Melanoma Res 18:322-329 https://doi.org/10.1097/CMR.0b013e32830b3536
  6. Cummins DL, Cummins JM, Pantle H, Silverman MA, Leonard AL, Chanmugam A. 2006. Cutaneous malignant melanoma. Mayo Clin Proc 81:500-507 https://doi.org/10.4065/81.4.500
  7. Green DR, Reed JC. 1998. Mitochondria and apoptosis. Science 281:1309-1312 https://doi.org/10.1126/science.281.5381.1309
  8. Guon TE, Chung HS. 2016. Effect of Zingiber officinale Roscoe extract on antioxidant and apoptosis in A2058 human melanoma cells. J East Asian Soc Diet Life 26:207-214 https://doi.org/10.17495/easdl.2016.6.26.3.207
  9. Igney FH, Krammer PH. 2002. Death and anti-death: Tumour resistance to apoptosis. Nat Rev Cancer 2:277-288 https://doi.org/10.1038/nrc776
  10. Kim EK, Choi EJ. 2015. Compromised MAPK signaling in human diseases: an update. Arch Toxicol 89:867-882 https://doi.org/10.1007/s00204-015-1472-2
  11. Kumar V, Abbas AK, Fausto N, Aster JC. 2010. Robins and Cotran: Pathologic Basis and Disease. 8th ed. p.25. Saunders
  12. Lee KH, Shin ES, Sim EJ, Bae YJ. 2020. Comparison of antioxidant and antimicrobial activities of fingerroot (Boesenbergia pandura) and ginger (Zingiber officinale Roscoe). Korean J Food Nutr 33:105-110 https://doi.org/10.9799/KSFAN.2020.33.1.105
  13. Lee SH, Meng XW, Flatten KS, Loegering DA, Kaufmann SH. 2013. Phosphatidylserine exposure during apoptosis reflects bidirectional trafficking between plasma membrane and cytoplasm. Cell Death Differ 20:64-76 https://doi.org/10.1038/cdd.2012.93
  14. Liu JF, Chen CY, Chen HT, Chang CS, Tang CH. 2016. BL-038, a benzofuran derivative, induces cell apoptosis in human chondrosarcoma cells through reactive oxygen species/mitochondrial dysfunction and the caspases dependent pathway. Int J Mol Sci 17:1491 https://doi.org/10.3390/ijms17091491
  15. Martinvalet D, Zhu P, Lieberman J. 2005. Granzyme A induces caspase-independent mitochondrial damage, a required first step for apoptosis. Immunity 22:355-370 https://doi.org/10.1016/j.immuni.2005.02.004
  16. Nakazawa T, Ohsawa K. 2002. Metabolism of [6]-gingerol in rats. Life Sci 70:2165-2175 https://doi.org/10.1016/S0024-3205(01)01551-X
  17. Park HY, Kim GY, Kwon TK, Hwang HJ, Kim ND, Yoo YH, Choi YH. 2013. Apoptosis induction of human leukemia U937 cells by 7,8-dihydroxyflavone hydrate through modulation of the Bcl-2 family of proteins and the MAPKs signaling pathway. Mutat Res Genet Toxicol Environ Mutagen 751:101-108 https://doi.org/10.1016/j.mrgentox.2012.12.002
  18. Pereira NA, Song Z. 2008. Some commonly used caspase substrates and inhibitors lack the specificity required to monitor individual caspase activity. Biochem Biophys Res Commun 377:873-877 https://doi.org/10.1016/j.bbrc.2008.10.101
  19. Pfeffer CM, Singh ATK. 2018. Apoptosis: A target for anticancer therapy. Int J Mol Sci 19:448 https://doi.org/10.3390/ijms19020448
  20. Portt L, Norman G, Clapp C, Greenwood M, Greenwood MT. 2011. Anti-apoptosis and cell survival: A review. Biochim Biophys Acta Mol Cell Res 1813:238-259 https://doi.org/10.1016/j.bbamcr.2010.10.010
  21. Rigel DS, Carucci JA. 2000. Malignant melanoma: Prevention, early detection, and treatment in the 21st century. CA Cancer J Clin 50:215-236 https://doi.org/10.3322/canjclin.50.4.215
  22. Rolt A, Cox LS. 2020. Structural basis of the anti-ageing effects of polyphenolics: Mitigation of oxidative stress. BMC Chem 14:50 https://doi.org/10.1186/s13065-020-00696-0
  23. Ryu HS. 2007. The effects of Zingiber officinale Roscoe extracts on mouse IFN-r and IL-10 production. Korean J Food Nutr 20:259-264
  24. Ryu MJ, Kim AD, Kang KA, Chung HS, Kim HS, Suh IS, Chang WY, Hyun JW. 2013. The green algae Ulva fasciataDelile extract induces apoptotic cell death in human colon cancer cells. In Vitro Cell Dev Biol Anim 49:74-81 https://doi.org/10.1007/s11626-012-9547-3
  25. Ryu MJ, Chung HS. 2015. [10]-Gingerol induces mitochondrial apoptosis through activation of MAPK pathway in HCT116 human colon cancer cells. In Vitro Cell Dev Biol Anim 51:92-101 https://doi.org/10.1007/s11626-014-9806-6
  26. Ryu MJ, Chung HS. 2016. Fucoidan reduces oxidative stress by regulating the gene expression of HO-1 and SOD-1 through the Nrf2/ERK signaling pathway in HaCaT cells. Mol Med Rep 14:3255-3260 https://doi.org/10.3892/mmr.2016.5623
  27. Yoshikawa M, Hatakeyama S, Chatani N, Nishino Y, Yamahara J. 1993. Qualitative and quantitative analysis of bioactive principles in Zingiberis rhizoma by means of high performance liquid chromatography and gas liquid chromatography. On the evaluation of Zingiberis rhizomaand chemical change of constituents during Zingiberis rhizoma processing. Yakugaku Zasshi 113:307-315 https://doi.org/10.1248/yakushi1947.113.4_307