The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Identification of an Unreported Fungal Species in Korea and Novel Ice Nucleation Active Fungus: Fusarium diversisporum

  • Diane Avalos-Ruiz (School of Applied Biosciences, Kyungpook National University) ;
  • Gwang-Jae Lim (School of Applied Biosciences, Kyungpook National University) ;
  • Seong-Keun Lim (School of Applied Biosciences, Kyungpook National University) ;
  • Leonid N. Ten (School of Applied Biosciences, Kyungpook National University) ;
  • In-Kyu Kang (Department of Horticultural Science, Kyungpook National University) ;
  • Seung-Yeol Lee (School of Applied Biosciences, Kyungpook National University) ;
  • Hee-Young Jung (School of Applied Biosciences, Kyungpook National University)
  • Received : 2022.08.23
  • Accepted : 2022.11.25
  • Published : 2022.12.31
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Abstract

In this study, the fungal strain KNUF-21-F39 was isolated from a declined apple tree (Malus domestica) in the Chungcheongbuk province in Korea. The strain KNUF-21-F39 presented a slow growth rate and a variety of macroconidia shapes and sizes ranging from ovoid to fusoid and 1- to 5-septate, primarily showing 3- and 4-septate, with "S" -shaped macroconidia rarely observed. The strain was identified based on morphological characteristics along with phylogenetic analysis performed using the internal transcribed spacer region (ITS) and partial sequences of translation elongation factor 1-α (tef1), RNA polymerase largest subunit (rpb1), and calmodulin (cal) genes. The fungal strain KNUF-21-F39 was identified as Fusarium diversisporum, which has not been previously reported in Korea. The ice nucleation activity (INA) of the strain was also evaluated, identifying the strain as positive for INA. This is the first report characterizing F. diversisporum as an IN-active fungal species.

Keywords

Acknowledgement

This work was carried out with the support of the Cooperative Research Program for Agriculture, Science and Technology Development (Project No. PJ014998022022), Rural Development Administration, Republic of Korea.

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