The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The effect of isoflavonoid contents in SH003 and its subfractions on breast cancer

SH003과 분획물의 구성 성분인 이소플라본의 유방암에 대한 효과

  • Yu-Jeong Choi (Department of Science in Korean Medicine, Graduate School, Kyung Hee University) ;
  • Won-Geun Choi (Department of Science in Korean Medicine, Graduate School, Kyung Hee University) ;
  • Kangwook Lee (Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University) ;
  • Miso Jeong (Department of Science in Korean Medicine, Graduate School, Kyung Hee University) ;
  • Sang Cheol Park (Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University) ;
  • Young Pyo Jang (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Seong-Gyu Ko (Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University)
  • 최유정 (경희대학교 일반대학원 기초한의과학과) ;
  • 최원근 (경희대학교 일반대학원 기초한의과학과) ;
  • 이강욱 (경희대학교 한의과대학 예방의학교실) ;
  • 정미소 (경희대학교 일반대학원 기초한의과학과) ;
  • 박상철 (경희대학교 일반대학원 나노의약생명과학과) ;
  • 장영표 (경희대학교 약학대학 한약학과) ;
  • 고성규 (경희대학교 한의과대학 예방의학교실)
  • Received : 2022.06.10
  • Accepted : 2022.08.16
  • Published : 2022.09.01
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Abstract

Objectives: We investigated the isoflavone contained in SH003 and its fractions, and the effect of these components on the inhibition of breast cancer. Methods: The isoflavones in solvent fractions of SH003 extract were identified by UPLC-MS and its contents were quantified using HPLC analysis. The estrogenic activity of SH003 or fractions was assessed by ERE luciferase assay in estrogen receptor (ER)-positive MCF-7 cells. To test the breast cancer inhibitory effect, the cell viability was measured using an MTT assay. Results: In this study, we demonstrated that SH003 and fractions contain 4 isoflavones which are calycosin-7-β-D-glucoside, formononetin-7-β-D-glucoside, calycosin, and formononetin. Despite containing isoflavones, estrogen-dependent transcription activity was not altered by both SH003 and fractions. On the other hand, SH003 and fractions inhibited the cell viability of breast cancer. In addition, its isoflavone components also showed reduced cell viability in various breast cancer cells. Conclusions: Overall, the phytoestrogen included in SH003 and fractions did not influence the estrogenic activity, emphasizing the safety of SH003 and fractions in breast cancer treatment.

Keywords

Acknowledgement

This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2020R1A5A2019413).

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