한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제26권1호
  • /
  • Pages.23-36
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  • 2022
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Expression and Functional Analysis of cofilin1-like in Craniofacial Development in Zebrafish

  • Jin, Sil (Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Jeon, Haewon (Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Choe, Chong Pyo (Division of Applied Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
  • 투고 : 2021.12.28
  • 심사 : 2022.03.09
  • 발행 : 2022.03.15
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초록

Pharyngeal pouches, a series of outgrowths of the pharyngeal endoderm, are a key epithelial structure governing facial skeleton development in vertebrates. Pouch formation is achieved through collective cell migration and rearrangement of pouch-forming cells controlled by actin cytoskeleton dynamics. While essential transcription factors and signaling molecules have been identified in pouch formation, regulators of actin cytoskeleton dynamics have not been reported yet in any vertebrates. Cofilin1-like (Cfl1l) is a fish-specific member of the Actin-depolymerizing factor (ADF)/Cofilin family, a critical regulator of actin cytoskeleton dynamics in eukaryotic cells. Here, we report the expression and function of cfl1l in pouch development in zebrafish. We first showed that fish cfl1l might be an ortholog of vertebrate adf, based on phylogenetic analysis of vertebrate adf and cfl genes. During pouch formation, cfl1l was expressed sequentially in the developing pouches but not in the posterior cell mass in which future pouch-forming cells are present. However, pouches, as well as facial cartilages whose development is dependent upon pouch formation, were unaffected by loss-of-function mutations in cfl1l. Although it could not be completely ruled out a possibility of a genetic redundancy of Cfl1l with other Cfls, our results suggest that the cfl1l expression in the developing pouches might be dispensable for regulating actin cytoskeleton dynamics in pouch-forming cells.

키워드

과제정보

We thank Jinsung Lim for technical assistance. This work was supported by Gyeongsang National University academic support (to H. J), by the Global Ph.D. Fellowship program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2019H1A2A1075288) (to S.J), and by a grant from Basic Science Research Program through the NRF funded by the Ministry of Science and ICT (2019R1A2C1004704) (to C.P.C).

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