한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제26권1호
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  • Pages.1-12
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  • 2022
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  • 2465-9525(pISSN)
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  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
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Signal Transduction of C-Terminal Phosphorylation Regions for Equine Luteinizing Hormone/Chorionic Gonadotropin Receptor (eLH/CGR)

  • Byambaragchaa, Munkhzaya (Institute of Genetic Engineering, Hankyong National University) ;
  • Joo, Hyo-Eun (Division of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University) ;
  • Kim, Sang-Gwon (Division of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University) ;
  • Kim, Yean-Ji (Division of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University) ;
  • Park, Gyeong-Eun (Division of Animal Science, School of Animal Life Convergence Sciences, Hankyong National University) ;
  • Min, Kwan-Sik (Institute of Genetic Engineering, Hankyong National University)
  • 투고 : 2021.12.03
  • 심사 : 2022.02.12
  • 발행 : 2022.03.15
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초록

This study aimed to investigate the signal transduction of phosphorylation sites at the carboxyl (C)-terminal region of equine luteinizing hormone/chorionic gonadotropin receptor (eLH/CGR). The eLH/CGR has a large extracellular domain of glycoprotein hormone receptors within the G protein-coupled receptors. We constructed a mutant (eLH/CGR-t656) of eLH/CGR, in which the C-terminal cytoplasmic tail was truncated at the Phe656 residue, through polymerase chain reaction. The eLH/CGR-t656 removed 14 potential phosphorylation sites in the intracellular C-terminal region. The plasmids were transfected into Chinese hamster ovary (CHO)-K1 and PathHunter Parental cells expressing β-arrestin, and agonist-induced cAMP responsiveness was analyzed. In CHO-K1 cells, those expressing eLH/CGR-t656 were lower than those expressing eLH/CGR wild-type (eLH/CGR-wt). The EC50 of the eLH/CGR-t656 mutant was approximately 72.2% of the expression observed in eLH/CGR-wt. The maximal response in eLH/CGR-t656 also decreased to approximately 43% of that observed in eLH/CGR-wt. However, in PathHunter Parental cells, cAMP activity and maximal response of the eLH/CGR-t656 mutant were approximately 173.5% and 100.8%, respectively, of that of eLH/CGR-wt. These results provide evidence that the signal transduction of C-terminal phosphorylation in eLH/CGR plays a pivotal role in CHO-K1 cells. The cAMP level was recovered in PathHunter Parental cells expressing β-arrestin. We suggest that the signal transduction of the C-terminal region phosphorylation sites is remarkably different depending on the cells expressing β-arrestin in CHO-K1 cells.

키워드

과제정보

This work was supported by a research grant from Hankyong National University in 2021.

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