Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Extraction of Natural Emulsifier from Medicago sativa L. and Sapindus saponaria L.: Optimization using CCD-RSM

알팔파 및 무환자나무열매로부터 천연유화제의 추출: CCD-RSM을 이용한 최적화

  • Hong, Seheum (Department of Polymer Science and Engineering, Dankook University) ;
  • Lee, Seung Bum (Department of Chemical Engineering, Dankook University)
  • 홍세흠 (단국대학교 고분자시스템공학부) ;
  • 이승범 (단국대학교 화학공학과)
  • Received : 2022.04.13
  • Accepted : 2022.05.09
  • Published : 2022.06.10
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Abstract

In this study, natural emulsifiers were extracted from Medicago sativa L. and Sapindus saponaria L. The extraction yield using CCD-RSM and the extraction process of foaming stability of the extracted product were optimized and 95% confidence interval was used to confirm the statistical reasonableness of the optimization. Herein, independent parameters were the ethanol volume and extraction temperature, whereas reaction parameters were the extraction yield and foaming stability. Under the condition of 53.5 vol% ethanol and extraction temperature (70.9 ℃), the maximum yield and foaming stability of the extracted product from Medicago sativa L were predicted as 26.2 wt% and 44.5%, respectively. In the case of the extraction from Sapindus saponaria L, the maximum yield and foaming stability were expected to be 31.9 wt% and 47.5% under the optimized conditions including 60.4 vol% of ethanol and extraction temperature (72.4 ℃). The average experimental error for validating the accuracy was about 3.4(± 0.3)% and 5.0(± 0.04)% for the extraction processes from Medicago sativa L. and Sapindus saponaria L., respectively.

본 연구에서는 알팔파 및 무환자나무열매로부터 천연유화제를 추출하여 CCD-RSM을 이용한 추출수율 및 추출물의 거품안정도에 관한 추출공정을 최적화하였으며, 95% 신뢰구간에서 최적화 결과의 통계학적 합리성을 확인하였다. 독립변수로는 주정/초순수의 부피비와 추출온도, 반응치로는 추출수율과 추출물의 거품안정도로 설정하였다. 주정/초순수 부피비 53.5 vol%, 추출온도 70.9 ℃인 최적조건 하에서 알팔파 추출공정의 최대 추출수율은 26.2 wt%, 추출물의 최대 거품안정도는 44.5%로 예측되었다. 무환자나무열매 추출공정의 경우, 최적조건인 주정/초순수 부피비 60.4 vol%, 추출온도 72.4 ℃에서 최대 추출수율은 31.9 wt%, 추출물의 최대 거품안정도는 47.5%로 예측되었다. 이러한 최적화 결과의 정확성을 확인하기 위해 실제 실험을 수행한 결과, 알팔파 및 무환자나무열매 추출공정에 대한 각각의 평균 오차율은 3.4(± 0.3)% 및 5.0(± 0.04)%로 나타났다.

Keywords

References

  1. K. Ogino and M. Abe, Mixed surfactant systems: Solution properties of anionic-nonionic mixed surfactant systems, Marcel Dekker, Inc., 1-23 (1992).
  2. E. Gawel and M. Grzelak, Lucerne(Medicago sativa L.) in the human diet-Case reports and short reports, J. Herb. Med., 10, 8-16 (2017). https://doi.org/10.1016/j.hermed.2017.07.002
  3. Z. Bialy, M. Jurzysta, W. Oleszek, S. Piacente, C. Pizza, and J. Agric, Saponins in Alfalfa (Medicago sativa L.) Root and Their Structural Elucidation, J. Agric. Food. Chem., 47, 3185-3192 (1999). https://doi.org/10.1021/jf9901237
  4. X. G. Liu, Y. Q. Sun, J. Bain, T. Han, D. D. Yue, D. Q. Li, and P. Y. Gao, Neuroprotective effects of triterpenoid saponins from Medicago sativa L. against H2O2-induced oxidative stress in SHSY5Y cells, Bioorg. Chem., 83, 468-476 (2019). https://doi.org/10.1016/j.bioorg.2018.11.008
  5. F. Rodrigues, P. D. Oliveria, J. Neves, B. Sarmento, M. H. Amaral, and M. B. Oliveira, Medicago spp. extracts as promising ingredients for skin care products, Ind. Crops Prod., 49, 634-644 (2013). https://doi.org/10.1016/j.indcrop.2013.06.015
  6. M. Homayoonfal, F. Khodaiyan, and M. Mousavi, Modelling and optimising of physicochemical features of walnut-oil beverage emulsions by implementation of response surface methodology: Effect of preparation conditions on emulsion stability, Food Chem., 174, 649-659 (2015). https://doi.org/10.1016/j.foodchem.2014.10.117
  7. B. S. Qader, E. E. Supeni, M. K. A. Ariffin, and A. R. A. Talib, RSM approach for modeling and optimization of designing parameters for inclined fins of solar air heater, Renew. Energy, 136, 48-68 (2019). https://doi.org/10.1016/j.renene.2018.12.099
  8. C. Yu, T. Cheng, J. Chen, Z. Ren, and M. Zeng, Investigation on thermal-hydraulic performance of parallel-flow shell and tube heat exchanger with a new type of anti-vibration baffle and wire coil using RSM method, Int. J. Therm. Sci., 138, 351-366 (2019). https://doi.org/10.1016/j.ijthermalsci.2018.12.035
  9. A. Garcia, S. A. Rhoden, J. Bernardi-Wenzel, R. C. Orlandelli, J. L. Azevedo, and J. A. Pamphile, Antimicrobial activity of crude extracts of endophytic fungi isolated from medicinal plant Sapindus saponaria L., J. Appl. Pharm. Sci., 2, 35-40 (2012).
  10. Z. Wang, J. Hong, L. Zhang, and P. Liu, Voltage fault detection and precaution of batteries based on entropy and standard deviation for electric vehicles, Energy Procedia, 105, 2163-2168 (2017). https://doi.org/10.1016/j.egypro.2017.03.611
  11. J. R. Pycke, Multivariate extensions of the Anderson-Darling process, Stat. Probabil. Lett., 63, 387-399 (2003). https://doi.org/10.1016/S0167-7152(03)00111-1