Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Nardotidis seu Sulculii Concha water extract (NSCE) on liver damage and depression in restraint-induced stress model

구속 스트레스 모델에서 석결명의 간손상 및 우울증 관련 인자에 미치는 영향

  • Kim, Min-Jung (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Oh, Tae-woo (Korean Institute of Oriental Medicine Technology Application Center) ;
  • Do, Hyun-joo (Daegu-gyeongbuk Medical innovation foundation) ;
  • Kim, Kwang-yeon (Korean Institute of Oriental Medicine Technology Application Center) ;
  • Yang, Joo-hye (Korean Institute of Oriental Medicine Technology Application Center) ;
  • Son, Jae-Dong (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Yang, Ye-jin (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University) ;
  • You, Young-Zoo (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Woo-Hyun (Department of Veterinary Epidemiology College of Veterinary Medicine, Gyeongsang National University) ;
  • Kang, Seung-Ho (Daegu Haany University) ;
  • Lee, Dong-ho (Department of Anaesthesiology and Pain Medicine, Busan Paik Hospital, Inje University) ;
  • Ki, Seung-hee (Department of Anaesthesiology and Pain Medicine, Busan Paik Hospital, Inje University) ;
  • Kim, Young-Woo (School of Korean Medicine, Dongguk University) ;
  • Park, Kwang-Il (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University)
  • 김민정 (경상대학교 수의과대학 수의생리학) ;
  • 오태우 (한국한의학연구원 한의기술응용센터) ;
  • 도현주 (대구경북첨단의료산업진흥재단) ;
  • 김광연 (한국한의학연구원 한의기술응용센터) ;
  • 양주혜 (한국한의학연구원 한의기술응용센터) ;
  • 손재동 (경상대학교 수의과대학 수의생리학) ;
  • 양예진 (경상대학교 수의과대학 수의생리학) ;
  • 유영주 (경상대학교 수의과대학 수의생리학) ;
  • 김우현 (경상대학교 수의과대학 수의전염병학) ;
  • 강승호 (대구한의대학교) ;
  • 이동호 (인제대학교 부산 백병원) ;
  • 기승희 (인제대학교 부산 백병원) ;
  • 김영우 (동국대학교 한의과대학) ;
  • 박광일 (경상대학교 수의과대학 수의생리학)
  • Received : 2022.05.10
  • Accepted : 2022.05.18
  • Published : 2022.05.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study investigated anti-inflammatory effects of Nardotidis seu Sulculii Concha water extract (NSCE) against restraint-induced stress. Methods : In vivo, NSCE was orally administered to male white mice at concentrations of 250 mg/kg and 500 mg/kg for 3 days, and then restraint-induced stress was induced for 6 hours. The level of liver damage was measured by serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). The stress-related hormones such as cortisol and corticosterone were measured by ELISA assay. Also, western blot analysis was performed to detect expression of mitogen-activated protein kinase (MAPK) and cyclooxygenase-2 (COX-2) proteins. Pathological changes were observed by hematoxylin and eosin (H&E) staining of the liver tissue, and Immunohistochemical (IHC) staining was performed to examine liver inflammation through macrophage infiltration. Results : The AST, ALT, LDH and the stress related hormones such as cortisol and corticosterone were significantly decreased in the NSCE treated group compared with stress group. In histological analysis, H&E staining of liver tissues did not detect the hepatic injury or damage in all groups. As a result of IHC staining, it was confirmed that infiltration of macrophages was increased in the stress-induced group, but decreased in the group treated with NSCE. The COX-2 and MAPK proteins expression was significantly increased by restraint-induced stress, but these proteins were decreased in the NSCE treated group. Conclusions : These results suggest that NSCE has the anti-inflammatory activity in restraint-induced stress model, and it is believed that NSCE can be used for the prevention of liver inflammation.

Keywords

Acknowledgement

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (number: HF20C0212) and (HF21C0061). This work was also supported by a grant (No. K17830) from the Korea Institute of Oriental Medicine funded by the Ministry of Education, Science and Technology (MEST), Republic of Korea.

References

  1. KIM JK. Mediation effect of LMX quality on the relationship between job stress and job satisfaction. The Journal of the Korea Contents Association. 2010;10(11):424-434. https://doi.org/10.5392/JKCA.2010.10.11.424
  2. Joung JY, Cho JH, Kim YH, Choi SH, Son CG. A literature review for the mechanisms of stress-induced liver injury. Brain Behavior. 2019;9(3):e01235. https://doi.org/10.1002/brb3.1235
  3. Russ TC, Kivimaki M, Morling JR, Starr JM, Stamatakis E, Batty GD. Association Between Psychological Distress and Liver Disease Mortality: A Meta-analysis of Individual Study Participants. Gastroenterology. 2015;148(5):958-66. https://doi.org/10.1053/j.gastro.2015.02.004
  4. Spiers JG, Steiger N, Khadka A, Juliani J, Hill AF, Lavidis NA, et al. Repeated acute stress modulates hepatic inflammation and markers of macrophage polarisation in the rat. Biochimie. 2021;(180): 30-42.
  5. Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, Feng Y. The role of oxidative stress and antioxidants in liver diseases. Itrernational journal of Molecular sciences. 2015;16:26087-124. https://doi.org/10.3390/ijms161125942
  6. Aube C, Bazeries P, Lebigot J, Cartier V, Boursier J. Liver fibrosis, cirrhosis, and cirrhosis-related nodules: Imaging diagnosis and surveillance. Diagn Interv Imaging. 2017 ;98(6):455-468. https://doi.org/10.1016/j.diii.2017.03.003
  7. Hong IH, Park SJ, Goo MJ, Lee HR, Park JK, Ki MR, Kim SH, Lee EM, Kim AY, Jeong KS. JNK1 and JNK2 regulate α-SMA in hepatic stellate cells during CCl4 -induced fibrosis in the rat liver. Pathology International. 2013;63:483-91. https://doi.org/10.1111/pin.12094
  8. Park HJ, Lee JS, Lee JD, Kim NJ, Pyo JH, Kang JM. The Anti-inflammatory Effect of Cinnamomi Ramulus. The Journal of Internal Korean Medicine. 2005;26:140-51.
  9. Kang SS. Medical Institute. Dictionary of Chinese Medical Herbs. Shanghai Science Publishing. 1998;2895-6.
  10. Moon SY, Kim YW, Kim SC. Anti-Inflammatory Effects of Haliotidis Concha. The Journal of Korean Medical Ophthalmology & Otolaryngology & Dermatology. 2013;26(4):70-80. https://doi.org/10.6114/jkood.2013.26.4.070
  11. Jeong DY, Kim YJ, Shim JS, Lee JH, Jung SK, Kim SY, et al. The Effect of Haliotidis Concha on the Growth and Ginsenoside Biosynthesis of Korean Ginseng Hairy Root. Journal of Ginseng Research. 2009;33:206-11. https://doi.org/10.5142/JGR.2009.33.3.206
  12. Kim KY, Lee SJ, Jee SY, Bae SJ, Song YR, Yun UJ, et al. Cellular-protective effects of Nardotidis seu Sulculii Concha Extract against oxidative stress. Herbal Formula Science. 2021;29(2):71-80. https://doi.org/10.14374/HFS.2021.29.2.71
  13. Yoo DH, Leeinchul. The Antioxidant Activity of Mangifera indica L. var. irwin Branches Extracts and the Inhibitoty Effect of Inflammatory Factors of iNOS, COX-2 in Macrophages. Journal of Investigative Cosmetology. 2020;16(4):357-65. https://doi.org/10.15810/JIC.2020.16.4.006
  14. Jew SS, Bae ON and Chung JH. Anti-inflammatory Effects of Asiaticoside on Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in RAW 264.7 Cell Line. Journal of toxicology and public health, an official journal of the Korean Society of Toxicology. 2003;19(1):33-37.
  15. Seibert K, Masferrer JL. Role of inducible cyclooxygenase (COX-2) in inflammation. Receptor. 1994;4(1):17-23.
  16. Lee HC, Yeom MJ, Kim GH, Han DO, Zhao MA, Shim IN et al. In vitro Study of Anti-inflammatory Effects of Salvia Miltiorrhiza Extracts Using Luciferase Reporter Gene Assay. Journal of Physiology & Pathology in Korean Medicine. 2004;18(3):740-746.
  17. Kunkel SL, Wiggins RC, Chensue SW, Larrick J. Regulation of macrophage tumor necrosis factor production by prostaglandin E2. Biochem Biophys Res Commun. 1986;137(1):404-10. https://doi.org/10.1016/0006-291X(86)91224-6
  18. Lo CJ, Cryer HG, Fu M, Lo FR. Regulation of macrophage eicosanoid generation is dependent on nuclear factor kappaB. Journal of Trauma. 1998;45(1):19-23. https://doi.org/10.1097/00005373-199807000-00004
  19. Maloney CG, Kutchera WA, Albertine KH, McIntyre TM, Prescott SM, Zimmerman GA. Inflammatory agonists induce cyclooxygenase type 2 expression by human neutrophils. Journal of Immunology. 1998;160(3):1402-10. https://doi.org/10.4049/jimmunol.160.3.1402
  20. Niiro H, Otsuka T, Izuhara K, Yamaoka K, Ohshima K, Tanabe T, et al. Regulation by interleukin-10 and interleukin-4 of cyclooxygenase-2 expression in human neutrophils. The Journal of the American Society of Hematology. 1997;(89)5:1621-1628.
  21. Guha M, Mackman M. LPS induction of gene expression in human monocytes. Elsevier. 2001;13:85-94.
  22. Mori M. Regulation of nitric oxide synthesis and apoptosis by arginase and arginine recyling. Journal of Nutrition. 2007;137:1616S-20S. https://doi.org/10.1093/jn/137.6.1616S
  23. Jang BC, Paik JH, Kim SP, Shin DH, Song DK, Park JG, et al. Catalase induced expression of inflammatory mediators via activation of NF-kappaB, PI3K/AKT, p70S6K, and JNKs in BV2 microglia. Cell Signal. 2005;17(5):625-33. https://doi.org/10.1016/j.cellsig.2004.10.001
  24. Johnson GL, Lapadat R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science. 2002;6;298(5600):1911-2. https://doi.org/10.1126/science.1072682
  25. Kim SK. Seo JM, Chae YR, Jung YS, Park JH, Kim YC. Alleviation of dimethylnitrosamine-induced liver injury and fibrosis betaine supplementation in rats. Chemico-Biological Interactions. 2009;177:204-211. https://doi.org/10.1016/j.cbi.2008.09.021
  26. Klein R, Nagy O, Tothova C, Chovanova F. Clinical and Diagnostic Significance of Lactate Dehydrogenase and Its Isoenzymes in Animals. Vet Med Int. 2020;2020:5346483.
  27. KIM SE. Optimal evaluation of the results of liver function tests. The Korean journal of medicine. 2019;94(1):89-95. https://doi.org/10.3904/kjm.2019.94.1.89
  28. Kim SZ, Jeong YM, Song WJ. A Temporary Increase of Liver Function Indicators, AST, ALT. Korea Genetics Research Center/KSZ Children's Hospital. 2013:13(1):43-47.
  29. Gong S, Miao YL, Jiao GZ, Sun MJ, Li H, Lin J, Luo MJ, et al. Dynamics and correlation of serum cortisol and corticosterone under different physiological or stressful conditions in mice. Public Library of Science. 2015;10(2):e0117503.
  30. Nam ES, Uhm DC. Effects of Phytoncides Inhalation on Serum Cortisol Level and Life Stress of College Students. Korean Journal of Adult Nursing. 2008;20(5):697-706.
  31. Lee HK. The Combined Effects of Treadmill Training and Black Garlic Intake on Cortiso Hormone, CK, CK-MB, LDH, Troponin T and Myoglobin in Rat. Pukyong National University. 2011.