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Isolation of Nine Bacteriophages Shown Effective against Erwinia amylovora in Korea

  • Park, Jungkum (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Byeori (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Song, Sujin (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Yong Whan (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Roh, Eunjung (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration)
  • 투고 : 2021.11.30
  • 심사 : 2022.04.19
  • 발행 : 2022.06.01

초록

Erwinia amylovora is a devastating bacterial plant pathogen that infects Rosaceae including apple and pear and causes fire blight. Bacteriophages have been considered as a biological control agent for preventing bacterial infections of plants. In this study, nine bacteriophages (ΦFifi011, ΦFifi044, ΦFifi051, ΦFifi067, ΦFifi106, ΦFifi287, ΦFifi318, ΦFifi450, and ΦFifi451) were isolated from soil and water samples in seven orchards with fire blight in Korea. The genetic diversity of bacteriophage isolates was confirmed through restriction fragment length polymorphism pattern analysis. Host range of the nine phages was tested against 45 E. amylovora strains and 14 E. pyrifoliae strains and nine other bacterial strains. Among the nine phages, ΦFifi044 and ΦFifi451 infected and lysed E. amylovora only. And the remaining seven phages infected both E. amylovora and E. pyrifoliae. The results suggest that the isolated phages were different from each other and effective to control E. amylovora, providing a basis to develop biological agents and utilizing phage cocktails.

키워드

과제정보

We thank Dr. Young-Kee Lee for providing the YKB strains of E. amylovora and E. pyrifoliae. This work was supported by the Rural Development Administration (RDA; grant number PJ01496501).

참고문헌

  1. Acimovic, S. G., Zeng, Q., McGhee, G. C., Sundin, G. W. and Wise, J. C. 2015. Control of fire blight (Erwinia amylovora) on apple trees with trunk-injected plant resistance inducers and antibiotics and assessment of induction of pathogenesis-related protein genes. Front. Plant Sci. 6:16. https://doi.org/10.3389/fpls.2015.00016
  2. Bereswill, S., Pahl, A., Bellemann, P., Zeller, W. and Geider, K. 1992. Sensitive and species-specific detection of Erwinia amylovora by polymerase chain reaction analysis. Appl. Environ. Microbiol. 58:3522-3526. https://doi.org/10.1128/aem.58.11.3522-3526.1992
  3. Besarab, N. V., Akhremchuk, A. E., Zlatohurska, M. A., Romaniuk, L. V., Valentovich, L. N., Tovkach, F. I., Lagonenko, A. L. and Evtushenkov, A. N. 2020. Isolation and characterization of Hena1 - a novel Erwinia amylovora bacteriophage. FEMS Microbiol. Lett. 367:fnaa070. https://doi.org/10.1093/femsle/fnaa070
  4. Born, Y., Fieseler, L., Marazzi, J., Lurz, R., Duffy, B. and Loessner, M. J. 2011. Novel virulent and broad-host-range Erwinia amylovora bacteriophages reveal a high degree of mosaicism and a relationship to Enterobacteriaceae phages. Appl. Environ. Microbiol. 77:5945-5954. https://doi.org/10.1128/AEM.03022-10
  5. Buttimer, C., Born, Y., Lucid, A., Loessner, M. J., Fieseler, L. and Coffey, A. 2018. Erwinia amylovora phage vB_EamM_Y3 represents another lineage of hairy Myoviridae. Res. Microbiol. 169:505-514. https://doi.org/10.1016/j.resmic.2018.04.006
  6. Dagher, F., Olishevska, S., Philion, V., Zheng, J. and Deziel, E. 2020. Development of a novel biological control agent targeting the phytopathogen Erwinia amylovora. Heliyon 6:e05222. https://doi.org/10.1016/j.heliyon.2020.e05222
  7. Doss, J., Culbertson, K., Hahn, D., Camacho, J. and Barekzi, N. 2017. A review of phage therapy against bacterial pathogens of aquatic and terrestrial organisms. Viruses 9:50. https://doi.org/10.3390/v9030050
  8. Dunne, M., Hupfeld, M., Klumpp, J. and Loessner, M. J. 2018. Molecular basis of bacterial host interactions by Gram-positive targeting bacteriophages. Viruses 10:397. https://doi.org/10.3390/v10080397
  9. Essa, N., Rossitto, M. and Fiscarelli, E. V. 2020. Phages and phage therapy: past, present and future. Microbiol. Med. 35:8709.
  10. Gayder, S., Parcey, M., Castle, A. J. and Svircev, A. M. 2019. Host range of bacteriophages against a world-wide collection of Erwinia amylovora determined using a quantitative PCR assay. Viruses 11:910. https://doi.org/10.3390/v11100910
  11. Gayder, S., Parcey, M., Nesbitt, D., Castle, A. J. and Svircev, A. M. 2020. Population dynamics between Erwinia amylovora, Pantoea agglomerans and bacteriophages: exploiting synergy and competition to improve phage cocktail efficacy. Microorganisms 8:1449. https://doi.org/10.3390/microorganisms8091449
  12. Ge, H., Hu, M., Zhao, G., Du, Y., Xu, N., Chen, X. and Jiao, X. 2020. The "fighting wisdom and bravery" of tailed phage and host in the process of adsorption. Microbiol. Res. 230:126344. https://doi.org/10.1016/j.micres.2019.126344
  13. Ham, H., Lee, Y.-K., Kong, H. G., Hong, S. J., Lee, K. J., Oh, G.-R., Lee, M.-H. and Lee, Y. H. 2020. Outbreak of fire blight of apple and Asian pear in 2015-2019 in Korea. Res. Plant Dis. 26:222-228 (in Korean). https://doi.org/10.5423/RPD.2020.26.4.222
  14. Kim, S. G., Lee, S. B., Giri, S. S., Kim, H. J., Kim, S. W., Kwon, J., Park, J., Roh, E. and Park, S. C. 2020. Characterization of novel Erwinia amylovora jumbo bacteriophages from Eneladusvirus genus. Viruses 12:1373. https://doi.org/10.3390/v12121373
  15. Kim, W.-S., Gardan, L., Rhim, S.-L. and Geider, K. 1999. Erwinia pyrifoliae sp. nov., a novel pathogen that affects Asian pear trees (Pyrus pyrifolia Nakai). Int. J. Syst. Bacteriol. 49:899-906. https://doi.org/10.1099/00207713-49-2-899
  16. Kim, W.-S., Hildebrand, M., Jock, S. and Geider, K. 2001. Molecular comparison of pathogenic bacteria from pear trees in Japan and the fire blight pathogen Erwinia amylovora. Microbiology 147:2951-2959. https://doi.org/10.1099/00221287-147-11-2951
  17. Lagonenko, A. L., Sadovskaya, O., Valentovich, L. N. and Evtushenkov, A. N. 2015. Characterization of a new ViI-like Erwinia amylovora bacteriophage phiEa2809. FEMS Microbiol. Lett. 362:fnv031.
  18. Lee, M. S., Lee, I., Kim, S. K., Oh, C.-S. and Park, D. H. 2018. In vitro screening of antibacterial agents for suppression of fire blight disease in Korea. Res. Plant Dis. 24:41-51 (in Korean). https://doi.org/10.5423/RPD.2018.24.1.41
  19. Loc-Carrillo, C. and Abedon, S. T. 2011. Pros and cons of phage therapy. Bacteriophage 1:111-114. https://doi.org/10.4161/bact.1.2.14590
  20. Mechan Llontop, M. E., Hurley, K., Tian, L., Bernal Galeano, V. A., Wildschutte, H. K., Marine, S. C., Yoder, K. S. and Vinatzer, B. A. 2020. Exploring rain as source of biological control agents for fire blight on apple. Front. Microbiol. 11:199. https://doi.org/10.3389/fmicb.2020.00199
  21. Mikicinski, A., Sobiczewski, P., Pulawska, J. and Maciorowski, R. 2016. Control of fire blight (Erwinia amylovora) by a novel strain 49M of Pseudomonas graminis from the phyllosphere of apple (Malus spp.). Eur. J. Plant Pathol. 145:265-276. https://doi.org/10.1007/s10658-015-0837-y
  22. Nagy, J. K., Schwarczinger, I., Kunstler, A., Pogany, M. and Kiraly, L. 2015. Penetration and translocation of Erwinia amylovora-specific bacteriophages in apple: a possibility of enhanced control of fire blight. Eur. J. Plant Pathol. 142:815-827. https://doi.org/10.1007/s10658-015-0654-3
  23. Park, D. H., Yu, J.-G., Oh, E.-J., Han, K.-S., Yea, M. C., Lee, S. J., Myung, I.-S., Shim, H. S. and Oh, C.-S. 2016. First report of fire blight disease on Asian pear caused by Erwinia amylovora in Korea. Plant Dis. 100:1946.
  24. Park, J., Lee, G. M., Kim, D., Park, D. H. and Oh, C.-S. 2018. Characterization of the lytic bacteriophage phiEaP-8 effective against both Erwinia amylovora and Erwinia pyrifoliae causing severe diseases in apple and pear. Plant Pathol. J. 34:445-450. https://doi.org/10.5423/PPJ.NT.06.2018.0100
  25. Principi, N., Silvestri, E. and Esposito, S. 2019. Advantages and limitations of bacteriophages for the treatment of bacterial infections. Front. Pharmacol. 10:513. https://doi.org/10.3389/fphar.2019.00513
  26. Ritchie, D. F. and Klos, E. J. 1977. Isolation of Erwinia amylovora bacteriophage from aerial parts of apple trees. Phytopathology 67:101-104. https://doi.org/10.1094/Phyto-67-101
  27. Sharifazizi, M., Harighi, B. and Sadeghi, A. 2017. Evaluation of biological control of Erwinia amylovora, causal agent of fire blight disease of pear by antagonistic bacteria. Biol. Control 104:28-34. https://doi.org/10.1016/j.biocontrol.2016.10.007
  28. Sharma, R., Pielstick, B. A., Bell, K. A., Nieman, T. B., Stubbs, O. A., Yeates, E. L., Baltrus, D. A. and Grose, J. H. 2019. A novel, highly related jumbo family of bacteriophages that were isolated against Erwinia. Front. Microbiol. 10:1533. https://doi.org/10.3389/fmicb.2019.01533
  29. Stone, E., Campbell, K., Grant, I. and McAuliffe, O. 2019. Understanding and exploiting phage-host interactions. Viruses 11:567. https://doi.org/10.3390/v11060567