The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of PCR-RFLP Technique for Identify Several Members of Fusarium incarnatum-equiseti Species Complex and Fusarium fujikuroi Species Complex

  • Pramunadipta, Syafiqa (Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Widiastuti, Ani (Departement of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Wibowo, Arif (Departement of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada) ;
  • Suga, Haruhisa (Life Science Research Center, Gifu University) ;
  • Priyatmojo, Achmadi (Departement of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada)
  • Received : 2021.12.22
  • Accepted : 2022.04.25
  • Published : 2022.06.01
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Abstract

Fusarium incarnatum-equiseti species complex (FIESC) contain over 40 members. The primer pair Smibo1FM/Semi1RM on the RPB2 partial gene has been reported to be able to identify Fusarium semitectum. The F. fujikuroi species complex (FFSC) contains more than 50 members. The F. verticillioides as a member of this complex can be identified by using VER1/VER2 primer pair on the CaM partial gene. In this research, the Smibo1FM/Semi1RM can amplify F. sulawesiense, F. hainanense, F. bubalinum, and F. tanahbumbuense, members of FIESC at 424 bp. The VER1/VER2 can amplify F. verticillioides, F. andiyazi, and F. pseudocircinatum, members of FFSC at 578 bp. Polymerase chain reaction-restriction fragment length polymorphism by using the combination of three restriction enzymes EcoRV, MspI, and HpyAV can differentiate each species of FIESC used. The two restriction enzymes HpaII and NspI can distinguish each species of FFSC used. The proper identification process is required for pathogen control in the field in order to reduce crop yield loss.

Keywords

Acknowledgement

This research was conducted as part of Syafiqa Pramunadipta's doctoral studies at Life Science Research Center, Gifu University as part of the "6-months Sandwich Program in UGSAS-GU 2019-2020" in research collaboration. The assistance of Mrs. Tomomi Katsu and Mrs. Ayako Usui are gratefully acknowledged. This research also funded by RTA UGM 2020.

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