The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of Angelicae Gigantis Radix for Inflammatory Response in HaCaT Cells

당귀(當歸) 추출물이 피부 각질형성세포의 염증반응에 미치는 영향

  • Huh, Jung (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Park, Hoyeon (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Eom Ji (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Kim, Eun-Young (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Sohn, Youngjoo (Department of Anatomy, College of Korean Medicine, Kyung Hee University) ;
  • Jung, Hyuk-Sang (Department of Anatomy, College of Korean Medicine, Kyung Hee University)
  • 허정 (경희대학교 한의과대학 해부학교실) ;
  • 박호연 (경희대학교 한의과대학 해부학교실) ;
  • 김엄지 (경희대학교 한의과대학 해부학교실) ;
  • 김은영 (경희대학교 한의과대학 해부학교실) ;
  • 손영주 (경희대학교 한의과대학 해부학교실) ;
  • 정혁상 (경희대학교 한의과대학 해부학교실)
  • Received : 2022.04.11
  • Accepted : 2022.05.25
  • Published : 2022.05.30
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Abstract

Objectives : Angelicae Gigantis Radix (AG) is a plant of the Ranunculus family. AG have been reported to have various pharmacological effects on human health which include uterine growth promotion, anti-inflammatory, analgesic, and immune enhancement. However, research on dermatitis disease is insufficient. Therefore, we investigated the effects of AG on tumor necrosis factor-α (TNF-α)/interferon-γ (IFN-γ) stimulated HaCaT cell. Methods : To investigate the effect of AG on HaCaT cell, HaCaT cells were pre-treated with AG for 1 hour and then stimulated with TNF-α/IFN-γ. After 24 hours, media and cells were harvested to analyze the inflammatory mediators. Concentration of human interleukin-1beta (IL-1β), monocyte chemoattractant protein-1 (MCP-1), granulocyte-macrophage colony-stimulating factor (GM-CSF), and TNF-α in the media were assessed by ELISA. mRNA expression of human thymus and activation-regulated chemokine (TARC), IL-6, and IL-8 were analyzed by RT-PCR. Additionally, the mechanisms of mitogen-activated protein kinases (MAPKs) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway were investigated by Western blot. Results : The treatment of AG inhibited gene expression levels of IL-6, IL-8, and TARC and protein expression levels of IL-1β, MCP-1, and GM-CSF. Also, AG significantly reduced extracellular signal-regulated kinase (ERK) phosphorylation and NF-κB translocation in TNF-α/IFN-γ stimulated HaCaT cell. Conclusions : Taken together, these results demonstrate that AG can alleviate inflammatory diseases such as atopic dermatitis by regulating the expression of inflammatory cytokines. Also, it suggest that AG may a promising candidate drug for the treatment of inflammatory disease such as atopic dermatitis.

Keywords

Acknowledgement

이 성과는 정부 (과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임 (No. 2020R1A2C2005836). 이 논문은 2021년도 정부 (교육부)의 재원으로 한국연구재단의 지원을 받아 수행된 기초연구사업임 (2021R1I1A1A01043143).

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