The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Single Oral Dose Toxicity Test of Lythri Herba Water Extracts in Sprague-Dawley Rat

털부처꽃 물 추출물의 Sprague-Dawley 랫드를 이용한 단회경구투여 독성시험

  • Kim, Hee-Young (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Park, Yea-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Shin, Su (Research Institute, Bio Port Korea Inc.) ;
  • Choi, Min-Woo (Research Institute, Bio Port Korea Inc.) ;
  • Hong, Eun-Jin (Research Institute, Bio Port Korea Inc.) ;
  • Shin, Young Woo (Daegu Catholic University GLP center) ;
  • Cha, Yun-Yeop (Department of Rehabilitative Medicine of Korean Medicine and Neuropsychiatry, College of Korean Medicine, Sangji University) ;
  • An, Hyo-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University)
  • 김희영 (상지대학교 한의과대학 약리학교실) ;
  • 박예진 (상지대학교 한의과대학 약리학교실) ;
  • 신수 ((주)바이오포트코리아 기업부설연구소) ;
  • 최민우 ((주)바이오포트코리아 기업부설연구소) ;
  • 홍은진 ((주)바이오포트코리아 기업부설연구소) ;
  • 신영우 (대구가톨릭대학교 GLP센터) ;
  • 차윤엽 (상지대학교 한의과대학 재활의학교실) ;
  • 안효진 (상지대학교 한의과대학 약리학교실)
  • Received : 2022.04.12
  • Accepted : 2022.05.25
  • Published : 2022.05.30
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Abstract

Objectives : Single oral dose toxicity test of Lythri Herba water extracts (LHWE) in Sprague-Dawley (SD) rat was performed to determine approximate lethal dose (ALD) of LHWE. Methods : This test was progressed according to OECD Guidelines for the Testing of Chemicals : acute oral toxicity. After adaptation of 7 days, SD rats were divided into 2 groups : vehicle control and 5000 mg/kg LHWE-treated group. Each group consisted of 5 female rats and 5 male rats. Vehicle or 5000 mg/kg LHWE was orally administrated once a day. Survival rates, general toxicity, and changes of body weight were investigated for 14 days after administration. On the last day of examination, the weight of all animals was measured and an autopsy was performed. All internal organ abnormalities were checked macroscopically and their findings were recorded. Results : In both groups, dead animals were not observed. During 14 days of administration, abnormal clinical signs were not detected. There was also no significant difference in weight gains between each group. Autopsy analysis showed that one case of the LHWE-treated female group had retention of clear fluid in the uterus; however, it was not considered to be affected by LHWE administration. Moreover, abnormal findings were not discovered in the control male group and the LHWE-treated male group. Conclusions : These results suggest that the ALD of LHWE exceed 5000 mg/kg and single oral administration of LHWE below 5000 mg/kg is nontoxic.

Keywords

Acknowledgement

본 연구는 농촌진흥청 연구사업(세부과제번호: PJ016132012022) 및 상지대학교 대학원 지원에 의하여 수행되었음

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