한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)

  • 제35권2호
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  • Pages.1-12
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  • 2022
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  • 1738-6640(pISSN)
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  • 2234-4020(eISSN)
대한한방안이비인후피부과학회 (The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology)
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산백국(山白菊) 열수추출물이 HaCaT 피부각질세포에서 미치는 항염증 효과

Effects of Aster glehni Water Extracts on Skin Inflammation in HaCaT Keratinocytes

  • 김세중 (상지대학교 한의과대학 안이비인후피부과학교실) ;
  • 이규영 (상지대학교 한의과대학 안이비인후피부과학교실) ;
  • 김근립 (상지대학교 한의과대학 안이비인후피부과학교실) ;
  • 홍철희 (상지대학교 한의과대학 안이비인후피부과학교실)
  • Kim, Se-Jung (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University) ;
  • Lee, Kyou-Young (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University) ;
  • Kim, Geun-Lip (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University) ;
  • Hong, Chul-Hee (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University)
  • 투고 : 2022.04.06
  • 심사 : 2022.04.26
  • 발행 : 2022.05.25
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초록

Objectives : This study was conducted to verify the anti-inflammatory effects of Aster glehni water extracts in HaCaT keratinocytes. Methods : In this study, cell viability was confirmed by MTT assay. Production of TNF-α and IL-6 was determined by ELISA. mRNA expression of TARC and MDC were measusred by qRT-PCR. Also, expressions of p-JNK, JNK, p-ERK, ERK, p-p38, and p-38 were investigated by using western blot assay. Results : Aster glehni water extracts were not shown any significant cytotoxicity at 15.625-500㎍/㎖ in HaCaT keratinocytes. Aster glehni extracts inhibited the TNF-α and IL-6 production in HaCaT keratinocytes treated with TNF-α and IFN-γ. Also, expression of TARC, MDC, p-ERK, and p-STAT1 was decreased. Conclusions : These results suggest that Aster glehni water extracts have anti-inflammatory effects in HaCaT keratinocytes and can be applied to the development of anti-inflammatory treatment substances.

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참고문헌

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