한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제67권1호
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  • Pages.41-52
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  • 2022
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  • 0252-9777(pISSN)
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  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
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RNA sequencing을 이용한 염 스트레스 처리 밀(Triticum aestivum)의 유전자 발현 차이 확인 및 후보 유전자 선발

Transcriptomic Analysis of Triticum aestivum under Salt Stress Reveals Change of Gene Expression

  • 전동현 (충남대학교 스마트농업시스템학과) ;
  • 임윤호 (충남대학교 식물자원학과) ;
  • 강유나 (충남대학교 농학과) ;
  • 박철수 (전북대학교 작물생명과학과) ;
  • 이동훈 (대전과학고등학교) ;
  • 박준찬 (대전과학고등학교) ;
  • 최우찬 (대전과학고등학교) ;
  • 김경훈 (농촌진흥청 국립식량과학원) ;
  • 김창수 (충남대학교 식물자원학과)
  • Jeon, Donghyun (Department of Science in Smart Agricultural Systems, Chungnam National University) ;
  • Lim, Yoonho (Department of Crop Science, Chungnam National University) ;
  • Kang, Yuna (Department of Crop Science, Chungnam National University) ;
  • Park, Chulsoo (Department of Crop Science and Biotechnology, Jeonju National University) ;
  • Lee, Donghoon (Daejeon Science High School for The Gifted) ;
  • Park, Junchan (Daejeon Science High School for The Gifted) ;
  • Choi, Uchan (Daejeon Science High School for The Gifted) ;
  • Kim, Kyeonghoon (National Institute of Crop Science) ;
  • Kim, Changsoo (Department of Crop Science, Chungnam National University)
  • 투고 : 2022.01.20
  • 심사 : 2022.02.08
  • 발행 : 2022.03.01
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초록

1. 본 연구에서는 우리밀 품종인 금강밀과 염 저항성을 가지는 돌연변이 라인 2020-s1340을 재료로 200 mM 염 스트레스 처리에 따른 전사체 발현을 확인하였다. QuantSeq을 통해 23,634,438개의 reads가 생산되었고 7,331,269개의 reads가 mapping됐다. 2. 염 스트레스 상황에서 총 282개의 DEG가 확인이 되었고 이러한 DEGs는 UDP-glucosyltransferase, receptor kinase-like protein, Lectin receptor-like kinases, cytochrome P450등의 단백질들을 코딩하는 유전자들이다. 이러한 DEGs는 염 저항성과 관련된 후보 유전자들이 될 수 있다. 염 저항성과 관련하여 역할이 밝혀지지 않은 유전자들은 추후 연구를 통해 확인이 필요하다. 3. GO연구에서는 DEGs를 세가지 범주로 분류하였으며 대부분 식물체 내 세포 기초 경로와 관련된 GO term들이 주로 되었으며 각각 범주에 있어서 biological process, molecular process에서는 single-organism process (GO: 0044699), single-organism metabolic process (GO:0044710), oxidation-reduction process (GO:0055114), copper ion transport (GO:0006825), copper ion transmembrane transport (GO:0035434), alternative oxidase activity (GO:0009916) GO term들이 유의성이 높게 나타났다. 4. 이러한 QuantSeq의 분석결과는 밀에 관한 염에 의해 발현되는 전사 발현에 대한 이해를 향상시킬 수 있다. 또한 염 스트레스 반응의 복잡한 분자 메커니즘에 대한 좋은 통찰력을 제공하고 염분 스트레스에 대한 작물 내성의 유전적 개선을 위한 실질적인 토대를 마련할 수 있을 것이다.

As a cultivar of Korean wheat, 'Keumgang' wheat variety has a fast growth period and can be grown stably. Hexaploid wheat (Triticum aestivum) has moderately high salt tolerance compared to tetraploid wheat (Triticum turgidum L.). However, the molecular mechanisms related to salt tolerance of hexaploid wheat have not been elucidated yet. In this study, the candidate genes related to salt tolerance were identified by investigating the genes that are differently expressed in Keumgang variety and examining salt tolerant mutation '2020-s1340.'. A total of 85,771,537 reads were obtained after quality filtering using NextSeq 500 Illumina sequencing technology. A total of 23,634,438 reads were aligned with the NCBI Campala Lr22a pseudomolecule v5 reference genome (Triticum aestivum). A total of 282 differentially expressed genes (DEGs) were identified in the two Triticum aestivum materials. These DEGs have functions, including salt tolerance related traits such as 'wall-associated receptor kinase-like 8', 'cytochrome P450', '6-phosphofructokinase 2'. In addition, the identified DEGs were classified into three categories, including biological process, molecular function, cellular component using gene ontology analysis. These DEGs were enriched significantly for terms such as the 'copper ion transport', 'oxidation-reduction process', 'alternative oxidase activity'. These results, which were obtained using RNA-seq analysis, will improve our understanding of salt tolerance of wheat. Moreover, this study will be a useful resource for breeding wheat varieties with improved salt tolerance using molecular breeding technology.

키워드

과제정보

본 연구는 농촌진흥청 국립식량과학원 농업공동연구(PJ01514904)의 지원을 받아 수행되었으며, 이에 감사드립니다.

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