Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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DsLCYB Directionally Modulated β-Carotene of the Green Alga Dunaliella salina under Red Light Stress

  • Yanhong Lan (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University) ;
  • Yao Song (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University) ;
  • Yihan Guo (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University) ;
  • Dairong Qiao (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University) ;
  • Yi Cao (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University) ;
  • Hui Xu (Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University)
  • Received : 2022.08.30
  • Accepted : 2022.10.24
  • Published : 2022.12.28
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Abstract

Carotenoids, which are natural pigments found abundantly in wide-ranging species, have diverse functions and high industrial potential. The carotenoid biosynthesis pathway is very complex and has multiple branches, while the accumulation of certain metabolites often affects other metabolites in this pathway. The DsLCYB gene that encodes lycopene cyclase was selected in this study to evaluate β-carotene production and the accumulation of β-carotene in the alga Dunaliella salina. Compared with the wild type, the transgenic algal species overexpressed the DsLCYB gene, resulting in a significant enhancement of the total carotenoid content, with the total amount reaching 8.46 mg/g for an increase of up to 1.26-fold. Interestingly, the production of α-carotene in the transformant was not significantly reduced. This result indicated that the regulation of DsLCYB on the metabolic flux distribution of carotenoid biosynthesis is directional. Moreover, the effects of different light-quality conditions on β-carotene production in D. salina strains were investigated. The results showed that the carotenoid components of β-carotene and β-cryptoxanthin were 1.8-fold and 1.23-fold higher than that in the wild type under red light stress, respectively. This suggests that the accumulation of β-carotene under red light conditions is potentially more profitable.

Keywords

Acknowledgement

This work was supported by National Natural Science Foundation of China (32171473, 32071479, 31670078), Fundamental Research Funds for the Central Universities (No.2018SCUH0072); Sichuan Science and Technology Bureau (2020YFH0076), Chengdu Science and Technology Bureau (2019-YF05-02317-SN, 2020-GH02-00037-HZ)." with "This work was supported by National Natural Science Foundation of China (32171473, 32071479, 32271535, 31670078), Fundamental Research Funds for the Central Universities (No.2018SCUH0072); Sichuan Science and Technology Bureau (2021YJ0024, 2022NSFSC0243, 2022NSFSC0119), Chengdu Science and Technology Bureau (2020-GH02-00037-HZ).

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