Journal of Ginseng Research

  • 제46권2호
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  • Pages.248-254
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  • 2022
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  • 1226-8453(pISSN)
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  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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Enhancing effect of Panax ginseng on Zip4-mediated zinc influx into the cytosol

  • Ikeda, Yoshito (Laboratory of Medicinal Cell Biology, Kobe Pharmaceutical University) ;
  • Munekane, Masayuki (Laboratory of Biophysical Chemistry, Kobe Pharmaceutical University) ;
  • Yamada, Yasuyuki (Laboratory of Medicinal Cell Biology, Kobe Pharmaceutical University) ;
  • Kawakami, Mizuki (Laboratory of Medicinal Cell Biology, Kobe Pharmaceutical University) ;
  • Amano, Ikuko (Laboratory of Medicinal Cell Biology, Kobe Pharmaceutical University) ;
  • Sano, Kohei (Laboratory of Biophysical Chemistry, Kobe Pharmaceutical University) ;
  • Mukai, Takahiro (Laboratory of Biophysical Chemistry, Kobe Pharmaceutical University) ;
  • Kambe, Taiho (Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University) ;
  • Shitan, Nobukazu (Laboratory of Medicinal Cell Biology, Kobe Pharmaceutical University)
  • 투고 : 2020.10.28
  • 심사 : 2021.06.09
  • 발행 : 2022.03.01
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초록

Background: Zinc homeostasis is essential for human health and is regulated by several zinc transporters including ZIP and ZnT. ZIP4 is expressed in the small intestine and is important for zinc absorption from the diet. We investigated in the present study the effects of Panax ginseng (P. ginseng) extract on modulating Zip4 expression and cellular zinc levels in mouse Hepa cells. Methods: Hepa cells were transfected with a luciferase reporter plasmid that contains metal-responsive elements, incubated with P. ginseng extract, and luciferase activity was measured. Using 65ZnCl2, zinc uptake in P. ginseng-treated cells was measured. The expression of Zip4 mRNA and protein in Hepa cells was also investigated. Finally, using a luciferase reporter assay system, the effects of several ginsenosides were monitored. Results: The luciferase activity in cells incubated with P. ginseng extract was significantly higher than that of control cells cultured in normal medium. Hepa cells treated with P. ginseng extract exhibited higher zinc uptake. P. ginseng extract induced Zip4 mRNA expression, which resulted in an enhancement of Zip4 protein expression. Furthermore, some ginsenosides, such as ginsenoside Rc and Re, enhanced luciferase activity driven by intracellular zinc levels. Conclusion: P. ginseng extract induced Zip4 expression at the mRNA and protein level and resulted in higher zinc uptake in Hepa cells. Some ginsenosides facilitated zinc influx. On the basis of these results, we suggest a novel effect of P. ginseng on Zip4-mediated zinc influx, which may provide a new strategy for preventing zinc deficiency.

키워드

과제정보

We thank Dr. Yumi Nishiyama and Yudai Hamaguchi (Kobe Pharmaceutical University, Japan) for help with the experiments. We thank Dr. Glen K. Andrews (University of Kansas Medical Center) for providing Hepa cells. The authors would like to thank Enago (www.enago.jp) for the English language review.

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