한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제37권2호
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  • Pages.63-68
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  • 2022
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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광주지역 유통·판매 수산물에서 분리된 장염비브리오의 독소유전자 분포 및 항생제 내성 조사

Distribution of Toxin Genes and Antimicrobial Resistance of Vibrio parahaemolyticus Isolated from Seafood in Gwangju

  • 정혜진 (광주광역시보건환경연구원) ;
  • 이민규 (광주광역시보건환경연구원) ;
  • 이향희 (광주광역시보건환경연구원) ;
  • 서시은 (광주광역시보건환경연구원) ;
  • 정소향 (광주광역시보건환경연구원) ;
  • 조배식 (광주광역시보건환경연구원) ;
  • 서정미 (광주광역시보건환경연구원)
  • Jeong, Hye Jin (Health and Environment Research Institute of Gwangju) ;
  • Lee, Min Gyou (Health and Environment Research Institute of Gwangju) ;
  • Lee, Hyang Hee (Health and Environment Research Institute of Gwangju) ;
  • Seo, Si Eun (Health and Environment Research Institute of Gwangju) ;
  • Jeong, So Hyang (Health and Environment Research Institute of Gwangju) ;
  • Cho, Bae Sik (Health and Environment Research Institute of Gwangju) ;
  • Seo, Jung Mi (Health and Environment Research Institute of Gwangju)
  • 투고 : 2022.02.25
  • 심사 : 2022.03.23
  • 발행 : 2022.04.30
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초록

본 연구에서는 광주광역시에 유통·판매되고 있는 수산물을 대상으로 식중독 원인균인 장염비브리오의 검출여부와 분리된 균주의 독소 특성 및 항생제 내성에 대하여 조사하였다. 패류 163건, 어류 97건 등을 포함하여 총 335건의 수산물 중 123건(36.7%)에서 장염비브리오가 검출되었다. 분리된 균주의 독소유전자 보유 여부를 확인한 결과 tdh 유전자는 모든 균주에서 검출되지 않았으며, trh 유전자는 패류 2건 및 갑각류 1건에서 분리된 3균주(2.4%)에서 검출되었다. 분리된 균주의 항생제 내성 실험 결과 116균주(94.3%)가 ampicillin에 대해 내성을 나타냈으며, ampicillin과 trimethoprim/sulfamethoxazole 2종류에 모두 내성을 나타낸 균주는 민어에서 분리된 1균주(0.8%)로 확인되었다. 이외에 amikacin, chloramphenicol 등을 포함한 9종의 항생제에 대해서는 모든 균주가 감수성을 나타낸 것으로 확인되었다. 따라서 본 연구 결과 광주지역 유통·판매 수산물에서 분리된 장염비브리오의 독소유전자 보유율 및 항생제 내성률은 비교적 낮으나, 환경적 요인에 의한 장염비브리오의 검출률 증가 및 항생제 내성균에 대한 주의가 필요함에 따라 수산물에 대한 지속적인 모니터링이 필요할 것으로 사료된다.

The purpose of this study is to investigate the distribution of toxin genes and antimicrobial resistance of Vibrio parahaemolyticus isolated from seafood in Gwangju. A total of 335 seafood, including 163 shellfish, 97 fish, and 36 mollusk, were tested in this study. As a result, V. parahaemolyticus was detected in 123 (36.7%) of 335 seafood. The tdh gene was not detected in all strains, while the trh gene was detected in 3 strains (2.4%). According to antimicrobial susceptibility test, 116 strains (94.3%) represent resistance to ampicillin, and 1 strain (0.8%) represents resistance to trimethoprim/sulfametoxazole. However, all strains were sensitive to 9 antimicrobial agents, including amikacin, chloramphenicol, tetracycline, and more. Therefore, the risk of V. parahaemolyticus isolated from seafood in Gwangju is considered low, but continuous monitoring of V. parahaemolyticus in seafood is required.

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참고문헌

  1. Daniels, N.A., MacKinnon, L., Bishop, R., Altekruse, S., Ray, B., Hammond, R.M., Thompson, S., Wilson, S., Bean, N.H., Griffin, P.N., Slutsker, L., Vibrio parahaemolyticus infections in the United States, 1973-1998. J. Infect. Dis., 181, 1661-1666 (2000). https://doi.org/10.1086/315459
  2. Ham, H.J., Jin, Y.H., Jung, Y.T., Distribution of Vibrio parahaemolyticus in fishery products, sold at Garak wholesale market and serological characteristics of isolated strains. J. Food Hyg. Saf., 17, 152-156 (2002).
  3. Su, Y.C., Liu, C., Vibrio parahaemolyticus: a concern of seafood safety. Food Microbiol., 24, 549-558 (2007). https://doi.org/10.1016/j.fm.2007.01.005
  4. Cho, E.D., Kim, H.D., Park, K.S., Antimicrobial resistance and minimum inhibitory concentrations of Vibrio parahaemolyticus strains isolated from seawater and commercial fisheries. Korean J. Fish Aquat. Sci., 52, 587-595 (2019). https://doi.org/10.5657/KFAS.2019.0587
  5. Ministry of Food and Drug Safety, (2022, January 3). Food poisoning statistics. Retrieved from https://www.foodsafetykorea.go.kr/portal/healthyfoodlife/foodPoisoningStat.do?menu_no=3724&menu_grp=MENU_NEW02
  6. Korea Rural Economic Institute, 2019. Food Balance Sheet. Korea Rural Economic Institute, Naju, Korea, pp. 24-33.
  7. Li, L., Meng, H., Gu, D., Li, Y., Jia, M., Molecular mechanisms of Vibrio parahaemolyticus pathogenesis. Microbiol. Res., 222, 43-51 (2019). https://doi.org/10.1016/j.micres.2019.03.003
  8. Yeung, P.M., Boor, K.J., Epidemioloty, Pathogenesis, and prevention of foodborne Vibrio parahaemolyticus infections. Foodborne Pathog. Dis., 1, 74-88 (2004). https://doi.org/10.1089/153531404323143594
  9. Letchumanan, V., Chan, K.G., Lee, L.H., Vibrio parahaemolyticus: a review on the pathogenesis, prevalence, and advance molecular identification techniques. Front. Microbiol., 5, 705 (2014). https://doi.org/10.3389/fmicb.2014.00705
  10. Nishibuchi, M., Taniguchi, T., Misawa, T., KhaeomaneeIam, V., Honda, T., Miwatani, T., Cloning and nucleotide sequence of the gene (trh) encoding the hemolysin related to the thermostable direct hemolysin of Vibrio parahaemolyticus. Infect. Immun., 57, 2691-2697 (1989). https://doi.org/10.1128/iai.57.9.2691-2697.1989
  11. Drake, S.L., DePaola, A., Jaykus, L.A., An Overview of Vibrio vulnificus and Vibrio parahaemolyticus. Compr. Rev. Food Sci. Food Saf., 6, 120-144 (2007). https://doi.org/10.1111/j.1541-4337.2007.00022.x
  12. Kim, T.O., Eum, I.S., Jo, S.M., Kim, H.D., Park, K.S., Antimicrobial-resistance profiles and virulence genes of Vibrio parahaemolyticus isolated from seawater in the Wando area. Korean J. Fish Aquat. Sci., 47, 220-226 (2014). https://doi.org/10.5657/KFAS.2014.0220
  13. Oh, E.G., Son, K.T., Yu, H., Lee, T.S., Lee H.J., Shin, S., Kwon, J.Y., Park K., Kim, J., Antimicrobial resistance of Vibrio parahaemolyticus and Vibrio alginolyticus strains isolated from farmed fish in Korea from 2005 through 2007. J. Food Prot., 74, 380-386 (2011). https://doi.org/10.4315/0362-028X.JFP-10-307
  14. National Institute of Food and Drug Safety Evaluation, Ministry of Agriculture, Food and Rural Affairs, Animal and Plant Quarantine Agency, 2019. Monitoring of antimicrobial use and antimicrobial resistance. Ministry of Agriculture, Food and Rural Affairs, Sejong, Korea, pp. 7-17.
  15. Ministry of Food and Drug Safety, 2020. Detection method for foodborne pathogens investigation, Cheongju, Korea, pp. 11-34.
  16. Ministry of Food and Drug Safety, 2020. Korea Food Code. Cheongju, Korea, pp. 263-323.
  17. Clinical and Laboratory Standards Institute. 2018. Performance standards for antimicrobial susceptibility testing, M100-S28. Wayne, PA, USA, pp. 30-52.
  18. Lee, H., Oh, Y.H., Choi, S.M., Park, S.G., Antibiotic susceptibility and distribution of Vibrio parahaemolyticus isolated from the seafood. J. Environ. Health Sci., 33, 16-20 (2007). https://doi.org/10.5668/JEHS.2007.33.1.016
  19. Park, K., Mok, J.S., Kwon, J.Y., Ryu, A.R., Shim, K.B., Seasonal and spatial variation of pathogenic Vibrio species isolated from seawater and shellfish off the Gyeongnam coast of Korea in 2013-2016. Korean J. Fish Aquat. Sci., 52, 27-34 (2019). https://doi.org/10.5657/KFAS.2019.0027
  20. Jeong, Y.I., Myung G.E., Choi, E.J., Soh, S.M., Park, G.J., Son, T.J., Distribution of pathogenic Vibrios in the aquatic environment adjacent to coastal areas of South Korea and analysis of the environmental factors affecting their occurrence. J. Environ. Health Sci., 44, 133-142 (2018).
  21. Go, Y.J., Jang, J.S., Relationships of pathogenic vibrios and environmental factors affecting their occurrence in the seawater of Incheon coastal area. Korean J. Food & Nutr. 26, 414-420 (2013). https://doi.org/10.9799/KSFAN.2013.26.3.414
  22. Yoon, C.Y., Kang, K.J., Occurrence of Vibrio parahaemolyticus in fishery products from the southwestern coast of Korea. Food Sci. Biotechnol., 15, 578-581 (2006).
  23. Yang, Z.Q., Jiao, X.A., Zhou, X.H., Cao, G.X., Fang, W.M., Gu, R.X., Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried, and salted seafood products in two coastal areas of eastern China. Int. J. Food Microbiol., 125, 279-285 (2008). https://doi.org/10.1016/j.ijfoodmicro.2008.04.007
  24. Wong, H.C., Chen, M.C., Liu, S.H., Liu, D.P., Incidence of highly genetically diversified Vibrio parahaemolyticus in seafood imported from Asian countries. Int. J. Food Microbiol., 52, 181-188 (1999). https://doi.org/10.1016/S0168-1605(99)00143-9
  25. Kim, W.B., Okuda, J., Matsumoto, C., Takahashi, N., Hashimoto, S., Nishibuchi, M., Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. J. Clin. Microbiol., 37, 1173-1177 (1999). https://doi.org/10.1128/JCM.37.4.1173-1177.1999
  26. Honda, T., NI, Y., Miwatani, T., Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin. Infect. Immun., 56, 961-965 (1988). https://doi.org/10.1128/iai.56.4.961-965.1988
  27. Park, Y.S., Park, K., Kwon, J.Y., Yu, H.S., Lee, H.J., Kim, J.H., Lee, T.S., Kim, P.H., Antimicrobial resistance and distribution of virulence factors of Vibrio parahaemolyticus isolated from shellfish farms on the southern coast of Korea. Korean J. Fish Aquat. Sci., 49, 460-466 (2016). https://doi.org/10.5657/KFAS.2016.0460
  28. Kim, S., An, S., Park, B., Oh, E.G., Song, K.C., Wan, K.J., Yu, H., Virulence factors and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from oyster Crassostrea gigas. Korean J. Fish Aquat. Sci., 49, 116-123 (2016). https://doi.org/10.5657/KFAS.2016.0116
  29. Robert-Pillot, A., Guenole, A., Lesne, J., Delesmont, R., Fournier, J.M., Quilici, M.L., Occurrence of the tdh and trh genes in Vibrio parahaemolyticus isolates from waters and raw shellfish collected in two French coastal areas and from seafood imported into France. Int. J. Food Microbiol., 91, 319-325 (2004). https://doi.org/10.1016/j.ijfoodmicro.2003.07.006
  30. Yu, H., Oh, E.G., Shin, S.B., Park, Y.S., Lee, H.J., Kin, J.H., Song, K.C., Distribution and antimicrobial resistance of Vibrio parahaemolyticus isolated from Korean shellfish. Korean J. Fish Aquat. Sci., 47, 508-515 (2014). https://doi.org/10.5657/KFAS.2014.0508
  31. Ryu, S.H., Hwang, Y.O., Park, S.G., Lee, Y.K., Antibiotic susceptibility of Vibrio parahaemolyticus isolated from commercial marine products. Korean J. Food Sci. Technol., 42, 508-513 (2010).
  32. Lopatek, M., Wieczorek, K., Osek, J., Antimicrobial resistance, virulence factors, and genetic profiles of Vibrio parahaemolyticus from seafood. Appl. Environ. Microbiol., 84, e00537-18 (2018).
  33. Odeyemi, O.A., Stratev, D., Occurrence of antimicrobial resistant or pathogenic Vibrio parahaemolyticus in seafood. A mini review. Revue Med. Vet., 167, 93-98 (2016).