BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Exercise-induced beige adipogenesis of iWAT in Cidea reporter mice

  • Kim, Jin Kyung (Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, BK21 Plus Program for Advanced Veterinary Science, Research Institute for Veterinary Science Seoul National University) ;
  • Go, Hye Sun (Korea Mouse Phenotyping Center (KMPC)) ;
  • Kim, Sol Pin (Korea Mouse Phenotyping Center (KMPC)) ;
  • Kim, Il Yong (Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, BK21 Plus Program for Advanced Veterinary Science, Research Institute for Veterinary Science Seoul National University) ;
  • Lee, Yun Hee (Korea Mouse Phenotyping Center (KMPC)) ;
  • Oh, Seung Hyun (Korea Mouse Phenotyping Center (KMPC)) ;
  • Lee, Ho (Korea Mouse Phenotyping Center (KMPC)) ;
  • Seong, Je Kyung (Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, BK21 Plus Program for Advanced Veterinary Science, Research Institute for Veterinary Science Seoul National University)
  • Received : 2021.09.16
  • Accepted : 2021.12.10
  • Published : 2022.04.30
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Abstract

Obesity is caused by an imbalance between energy intake and energy expenditure. Exercise is attracting attention as one of the ways to treat obesity. Exercise induces 'beige adipogenesis' in white adipose tissue, increasing total energy expenditure via energy dissipation in the form of heat. Also, beige adipogenesis can be induced by treatment with a beta-adrenergic receptor agonist. We developed a Cidea-dual reporter mouse (Cidea-P2A-Luc2-T2A-tdTomato, Luciferase/tdTomato) model to trace and measure beige adipogenesis in vivo. As a result, both exercise and injection of beta-adrenergic receptor agonist induced beige adipogenesis and was detected through fluorescence and luminescence. We confirmed that exercise and beta-adrenergic receptor agonist induce beige adipogenesis in Cidea-dual reporter mouse, which will be widely used for detecting beige adipogenesis in vivo.

Keywords

Acknowledgement

This study was partially supported by the Research Institute for Veterinary Science, Seoul National University.

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