한국환경농학회지 (Korean Journal of Environmental Agriculture)

  • 제41권4호
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  • Pages.335-343
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  • 2022
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  • 1225-3537(pISSN)
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  • 2233-4173(eISSN)
한국환경농학회 (The Korean Society of Environmental Agriculture)
권호 표지
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사람 및 가축 유래 분변 미생물 군집과 항생제 내성 유전자 간 상관 관계에 대한 연구

Co-occurrence Analyses of Antibiotic Resistance Genes and Microbial Community in Human and Livestock Animal Feces

  • 정지원 (제주대학교 생명자원과학대학 분자생명공학전공(생명공학부)) ;
  • 반다리 아프라지타 (제주대학교 생명자원과학대학 분자생명공학전공(생명공학부)) ;
  • 운노 타쯔야 (제주대학교 생명자원과학대학 분자생명공학전공(생명공학부))
  • Jiwon Jeong (Molecular Biotechnology (Faculty of Biotechnology), College of Applied Life Sciences, Jeju National University) ;
  • Aprajita Bhandari (Molecular Biotechnology (Faculty of Biotechnology), College of Applied Life Sciences, Jeju National University) ;
  • Tatsuya Unno (Molecular Biotechnology (Faculty of Biotechnology), College of Applied Life Sciences, Jeju National University)
  • 투고 : 2022.12.15
  • 심사 : 2022.12.21
  • 발행 : 2022.12.31
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초록

BACKGROUND: Antibiotics used in animal husbandry for disease prevention and treatment have resulted in the rapid progression of antibiotic resistant bacteria which can be introduced into the environment through livestock feces/manure, disseminating antibiotic resistant genes (ARGs). In this study, fecal samples were collected from the livestock farms located in Jeju Island to investigate the relationship between microbial communities and ARGs. METHODS AND RESULTS: Illumina MiSeq sequencing was applied to characterize microbial communities within each fecal sample. Using quantitative PCR (qPCR), ten ARGs encoding tetracycline resistance (tetB, tetM), sulfonamide resistance (sul1, sul2), fluoroquinolone resistance (qnrD, qnrS), fluoroquinolone and aminoglycoside resistance (aac(6')-Ib), beta-lactam resistance (blaTEM, blaCTX-M), macrolide resistance (ermC), a class 1 integronsintegrase gene (intI1), and a class 2 integrons-integrase gene (intI2) were quantified. The results showed that Firmicutes and Bacteroidetes were dominant in human, cow, horse, and pig groups, while Firmicutes and Actinobacteria were dominant in chicken group. Among ARGs, tetM was detected with the highest number of copies, followed by sul1 and sul2. Most of the genera belonging to Firmicutes showed positive correlations with ARGs and integron genes. There were 97, 34, 31, 25, and 22 genera in chicken, cow, pig, human, and horse respectively which showed positive correlations with ARGs and integron genes. In network analysis, we identified diversity of microbial communities which correlated with ARGs and integron genes. CONCLUSION(S): In this study, antibiotic resistance patterns in human and livestock fecal samples were identified. The abundance of ARGs and integron genes detected in the samples were associated with the amount of antibiotics commonly used for human and livestocks. We found diverse microbial communities associated with antibiotics resistance genes in different hosts, suggesting that antibiotics resistance can disseminate across environments through various routes. Identifying the routes of ARG dissemination in the environment would be the first step to overcome the challenge of antibiotic resistance in the future.

키워드

과제정보

This research was supported by the 2022 scientific promotion program funded by Jeju National University.

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