Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Inhibition of Lipase Activity and Preadipocyte Differentiation in 3T3-L1 Cells Treated with Sargassum horneri Extract

괭생이모자반 추출물의 리파아제 저해 활성 및 3T3-L1 지방전구세포 분화억제 효과

  • Hong, Ji Woo (Department of Food Science, Sunmoon University) ;
  • Park, Ha Young (Department of Food Science, Sunmoon University) ;
  • Park, Jae Hyun (Department of Food Science, Sunmoon University) ;
  • Kim, So Hee (Department of Food Science, Sunmoon University) ;
  • Kim, Han A (Department of Food Science, Sunmoon University) ;
  • Kim, Jin-Woo (Department of Food Science, Sunmoon University)
  • 홍지우 (선문대학교 건강보건대학 식품과학과) ;
  • 박하영 (선문대학교 건강보건대학 식품과학과) ;
  • 박재현 (선문대학교 건강보건대학 식품과학과) ;
  • 김소희 (선문대학교 건강보건대학 식품과학과) ;
  • 김한아 (선문대학교 건강보건대학 식품과학과) ;
  • 김진우 (선문대학교 건강보건대학 식품과학과)
  • Received : 2022.01.04
  • Accepted : 2022.02.25
  • Published : 2022.03.30
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Abstract

In this study, in order to evaluate the anti-obesity effect of sargassum horneri extract, the effects of the extract on lipase activity and preadipocyte differentiation in 3T3-L1 cells were investigated. S. horneri extract between 0.0 and 1.0 mg/mL showed no cytotoxicity and inhibited lipase activity by 68.1%. When S. horneri extract was utilized at levels of 0.25, 0.5, and 1.0 mg/mL in 3T3-L1 cells, preadipocytes differentiation decreased by 11.4, 19.7, and 25.6%, respectively, showing anti-obesity effects. In addition, after treatment with 1.0 mg/mL S. horneri extract, the mRNA expression levels of sterol regulatory element binding proteins-1c (SREBP-1c), peroxisome proliferator activated receptor-γ (PPAR-γ), CCAAT enhancer binding protein-α (CEBP-α), fatty acid synthase (FAS), and stearoyl-CoA desaturase1 (SCD1) in 3T3-L1 cells were significantly decreased (p < 0.05) by 65.2, 54.9, 50.0, 33.8, and 33.8% respectively. These results showed that S. horneri extract suppresses lipase activity and prophylactic preadipocyte differentiation in 3T3-L1, and thus can be used as an anti-obesity agent in functional foods and medicines.

Keywords

Acknowledgement

연구에 사용된 시료를 제공해주신 김지훈 박사님께 감사드립니다.

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