Animal Bioscience

  • 제35권5호
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  • Pages.670-676
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  • 2022
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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The efficiency of different types of extenders for semen cooling in stallions

  • Reckova, Zuzana (Department of Animal Breeding, Mendel University in Brno (FA)) ;
  • Filipcík, Radek (Department of Animal Breeding, Mendel University in Brno (FA)) ;
  • Souskova, Katarína (Department of Animal Breeding, Mendel University in Brno (FA)) ;
  • Kopec, Tomas (Department of Animal Breeding, Mendel University in Brno (FA)) ;
  • Hosek, Martin (Department of Animal Breeding, Mendel University in Brno (FA)) ;
  • Pesan, Vojtech (Department of Animal Breeding, Mendel University in Brno (FA))
  • 투고 : 2021.06.28
  • 심사 : 2021.12.07
  • 발행 : 2022.05.01
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초록

Objective: This study was conducted to examine influence of skimmed milk-based extender (SM), INRA 96 extender and BotuSemen Gold extender on parameters of stallions' ejaculate during storage. Methods: In this study, 14 stallions between 4 and 20 years of age were monitored. Total and progressive motility, viability and morphology of sperm were evaluated at time intervals of 24, 48, and 72 hours after collection. Results: The total motility, progressive motility, and values of sperm with normal morphology were significantly higher in the INRA 96 and BotuSemen Gold extenders than in the SM (p<0.01). The sperm viability differed significantly in all extenders (p<0.01). The highest value of sperm viability was in INRA 96 (64.69%±0.67%) and lowest in SM (59.70%±0.81%). The highest differences occurred at 72 hours of storage. Values of total motility, progressive motility and sperm viability decreased over time (p<0.01). In case of sperm morphology there was no statistically significant decrease between 48- and 72-hour time intervals. Conclusion: It can be concluded that the extenders with a chemically defined composition have shown better indicators of insemination capabilities in ejaculates than the SM. The BotuSemen Gold extender is a suitable alternative to the INRA 96, when used within 48 hours; after 72 hours of storage, however, the INRA 96 showed a higher share of viable spermatozoa.

키워드

과제정보

This research was funded by AF-IGA2020-IP066.

참고문헌

  1. Aurich JE. Artificial insemination in horses-more than a century of practice and research. J Equine Vet Sci 2012;32:458-63. https://doi.org/10.1016/j.jevs.2012.06.011
  2. Kowalczyk A, Czerniawska-Piatkowska E, Kuczaj M. Factors influencing the popularity of artificial insemination of mares in Europe. Animals 2019;9:460. https://doi.org/10.3390/ani9070460
  3. LeFrapper L, Walston L, Whisnant CS. Comparison of various extenders for storage of cooled stallion spermatozoa for 72 hours. J Equine Vet Sci 2010;30:200-4. https://doi.org/10.1016/j.jevs.2010.02.007
  4. Coutinho da Silva MA, Seidel Jr GE, Squires EL, Graham JK, Carnevale EM. Effects of components of semen extenders on the binding of stallion spermatozoa to bovine or equine zonae pellucidae. Reproduction 2012;143:577-85. https://doi.org/10.1530/REP-11-0099
  5. Aurich Ch. Recent advances in cooled-semen technology. Anim Reprod Sci 2008;107:268-75. https://doi.org/10.1016/j.anireprosci.2008.04.015
  6. Batellier F, Vidament M, Fauquant J, et al. Advances in cooled semen technology. Anim Reprod Sci 2001;68:181-90. https://doi.org/10.1016/S0378-4320(01)00155-5
  7. Hernandez-Aviles C, Love ChC, Serafini R, et al. Effects of glucose concentration in semen extender and storage temperature on stallion sperm quality following long-term cooled storage. Theriogenology 2020;147:1-9. https://doi.org/10.1016/j.theriogenology.2020.02.007
  8. Extenders. Animal Reproduction Systems [cited 2021 June 18]. Available from: https://www.arssales.com/extenders.html
  9. Pagl R, Aurich JE, Muller-Schlosser F, Kankofer M, Aurich Ch. Comparison of an extender containing defined milk protein fractions with a skim milk-based extender for storage of equine semen at 5℃. Theriogenology 2006;66:1115-22. https://doi.org/10.1016/j.theriogenology.2006.03.006
  10. Batellier F, Magistrini M, Fauquant J, Palmer E. Effect of milk fractions on survival of equine spermatozoa. Theriogenology 1997;48:391-410. https://doi.org/10.1016/S0093-691X(97)00250-1
  11. Batellier F, Gerard N, Courtens JL, Palmer E, Magistrini M. Preservation of stallion sperm quality by native phosphocaseinate: a direct or indirect effect? J Reprod Fertil Supplement 2000;56:69-77.
  12. Novello G, Podico G, Segabinazzi LGTM, Lima FS, Canisso IF. Stallion semen cooling using native phosphocaseinate-based extender and sodium caseinate cholesterol-loaded cyclodextrin-based extender. J Equine Vet Sci 2020;92:103104. https://doi.org/10.1016/j.jevs.2020.103104
  13. Bergeron A, Manjunath P. New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Mol Reprod Dev 2006;73:1338-44. https://doi.org/10.1002/mrd.20565
  14. Manjunath P. New insights into the understanding of the mechanism of sperm protection by extender components. Anim Reprod 2012;9:809-15.
  15. Hartwig FP, Lisboa FP, Hartwig FP, et al. Use of cholesterol-loaded cyclodextrin: an alternative for bad cooler stallions. Theriogenology 2014;81:340-6. https://doi.org/10.1016/j.theriogenology.2013.10.003
  16. Mamica O, Havlickova T, Reckova Z. Analysis of stallion sperm survival in refrigerated insemination doses using different types of sperm diluents. Proceedings of the Current problems of horse breeding in the Czech Republic, Brno, Czech Republic, 8.11.2019; Sobotkova, E.; Mendel University in Brno, 2019.
  17. Freitas M, Boures CS, Jos Gonaves de Oliviera F, Oliveira RA. The effect of skim milk as an equine semen extender. Proceedings of the ADSA-ASAS-CSAS Joint Annual Meeting; 2014 July 20-4: Kansas City, MO, USA.
  18. Martecikova S, Hulinska P, Reckova Z, Pavlik A, Jeseta M, Machatkova M. Effect of acrosome reaction progress in frozenthawed boar spermatozoa on the efficiency of in vitro oocyte fertilization. Vet Med 2010;55:429-37. https://doi.org/10.17221/2985-VETMED
  19. Cordova A, Strobel P, Vallejo A, et al. Use of hypometabolic TRIS extenders and high cooling rate refrigeration for cryopreservation of stallion sperm: Presence and sensitivity of 5' AMP-activated protein kinase (AMPK). Cryobiology 2014;69:473-81. https://doi.org/10.1016/j.cryobiol.2014.10.008
  20. Veznik Z, Svecova D, Zajicova A, et al. Repetitorium of spermatology and andrology, methods of spermatoanalysis; Research Institute of Veterinary Medicine, Brno, Czech Republic, 2004. pp. 97-9.
  21. Province CA, Squires EL, Pickett BW, Amann RP. Cooling rates, storage temperatures and fertility of extended equine spermatozoa. Theriogenology 1985;23:925-34. https://doi.org/10.1016/0093-691X(85)90010-X
  22. Brisko SP, Crockett EC, Squires EL. Effect of centrifugation and partial removal of seminal plasma on equine spermatozoa motility after cooling and storage. Theriogenology 2000;54:129-36. https://doi.org/10.1016/S0093-691X(00)00331-9
  23. Vidament M, Magistrini M, Le Foll Y, et al. Temperatures from 4 to 15 ℃ are suitable for preserving the fertilizing capacity of stallion semen stored for 22 h or more in INRA96 extender. Theriogenology 2012;78:297-307. https://doi.org/10.1016/j.theriogenology.2012.01.018
  24. Aurich Ch. Factors affecting the plasma membrane function of cooled-stored stallion spermatozoa. Anim Reprod Sci 2005;89:65-75. https://doi.org/10.1016/j.anireprosci.2005.06.025
  25. Merkies K, Chenier T, Plante C, Buhr MM. Assessment of stallion spermatozoa viability by flow cytometry and light microscope analysis. Theriogenology 2000;54:1215-24. https://doi.org/10.1016/S0093-691X(00)00428-3
  26. Saunders WB. Fertility and infertility in male animals. In: Noakes DE, Parkinson TJ, England GCW, Arthur GH, editors. Arthur's veterinary reproduction and obstetrics, 8th ed. London, UK: WB Saunders; 2001. pp. 695-750.
  27. Batellier F, Duchamp G, Vidament M, Arnaud G, Palmer E, Magistrini M. Delayed insemination is successful with new extender for storing fresh equine semen at 15℃ under aerobic condition. Theriogenology 1998;50:229-36. https://doi.org/10.1016/s0093-691x(98)00130-7
  28. Varner DD. Developments in stallion semen evaluation. Theriogenology 2008;70:448-62. https://doi.org/10.1016/j.theriogenology.2008.04.023
  29. Tharasanit T, Manee-In S, Khamenkhetwit P, Sirivaidyapong S, Lohachit C. The effect of cold storage on the quality of stallion semen and pregnancy rate after artificial insemination. Thai J Vet Med 2007;37:39-48.