대한의생명과학회지 (Biomedical Science Letters)

  • 제28권1호
  • /
  • Pages.34-41
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  • 2022
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
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Process Optimization of Nypa fruticans Wurmb Extract using Mixed Solvent and its Functional Component Analysis

  • Kim, Jin (Dental Healthcare & Clinical Trial Center, Chosun University) ;
  • Kim, Han Sung (Him Co., Ltd, Business Incubation Center, Chosun University) ;
  • Son, Jang Wan (School of Infomatics & Product Design, Chosun University) ;
  • Moon, Seong Yong (Dental Healthcare & Clinical Trial Center, Chosun University) ;
  • Lee, Sook-Young (Marine Bio Research Center, Chosun University)
  • 투고 : 2022.02.10
  • 심사 : 2022.03.30
  • 발행 : 2022.03.31
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초록

In this study, conditions for extraction of functional component from Nypa fruticans wurmb was optimized. The yield by extraction with 30% ethanol (LE30, 42.12%) was higher than those hot water extraction (LDW 33.32%), 50% ethanol (LE50, 40.12%) and 70% ethanol (LE70, 34.5%). The extract was purified and analyzed by GC MS. The prevailing compounds found in extract were Cyclodecasiloxane-, pentadecanoic acid, -eicosane, undecanal and tridecanoic acid. The presence of saturated and unsaturated fatty acids in ethanolic extract vindicate the use of this plant to treat many diseases in traditional medicine. The total phenolic contents in the LDW, LE30, LE50, LE70 extract were 128±1.65 mg/g, 205±2.3 mg/g, 210±4.23 mg/g and 180±5.6 mg/g, respectively. The DPPH was highest in LE70 extract (1,000 ㎍/mL, 81.14%), ABTS was highest in LE50 extract (1,000 ㎍/mL, 84.14%). The protective effects against oxidative stress in raw 264.7 cell imparted by the LE50 extract was better than those imparted by the other extracts. The findings of the present study suggest that 50% ethanol is best solvent for extraction of Nypa fruticans Wurmb, considering yield, polyphenol content, and antioxidant activities with extraction cost.

키워드

과제정보

Following are results of a study on the "Leaders in INdustry-university Cooperation +" Project, supported by the Ministry of Education and National Research Foundation of Korea.

참고문헌

  1. Andrzej L, Olszowy DM. The importance of solvent type in estimating antioxidant properties of phenolic compounds by ABTS assay. European Food Research and Technology. 2013. 236: 1099-1105. https://doi.org/10.1007/s00217-013-1982-1
  2. Bi J, Jiang B, Liu JH, Lei C, Zhang XL, An LJ. Protective effects of catalpol against H2O2-induced oxidative stress in astrocytes primary cultures. Neuroscience Letters. 2008. 442: 224-227. https://doi.org/10.1016/j.neulet.2008.07.029
  3. Bobade AF. GC-MS Analysis of bioactive compound in ethanolic extract of Pithecellobium dulce leaves. American Society of Plastic Surgeons. 2019. 3: 8-13.
  4. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958. 181: 1199-1200. https://doi.org/10.1038/1811199a0
  5. Emi II, Yoichi K, Ikuko M, Masato A, Norio O. Glial Cells Protect Neurons Against Oxidative Stress via Transcriptional Up-Regulation of the Glutathione Synthesis. Journal of Neurochemistry. 2002. 72: 2334-2344. https://doi.org/10.1046/j.1471-4159.1999.0722334.x
  6. Hwang YG, Lee JJ, Kim AR, Lee MY. Chemical Components and antioxidative effects of Eriobotrya japonica Lindl. Leaf. International Journal of Life Sciences. 2010. 20: 1625-1633.
  7. Kim IR. Antioxidant and Pro-oxidant Activities of Hamcho (Salicornia herbacea L.). The Korean Journal of Food and Nutrition. 2015. 28: 40-46. https://doi.org/10.9799/KSFAN.2015.28.1.040
  8. Lee JJ, Park DH, Lee WY. Optimization of microwave-assisted extraction process of Hordeum vulgare L.by response surface methodology. Korean Joutnal of Food Preservation. 2017. 24: 949-956. https://doi.org/10.11002/kjfp.2017.24.7.949
  9. Kim SN, Kim MR, Cho SM, Kim SY, Kim JB, Cho YS. Antioxidant activities and determination of phenolic compounds isolated from oriental plums (soldam, oishiwase and formosa). Nutrition Research and Practice. 2012. 6: 277-285. https://doi.org/10.4162/nrp.2012.6.4.277
  10. Koffi E, Sea T, Dodeh Y, Soro S. Effect of solvent type on extraction of polyphenols from twenty three ivorian plants. J Anim Plant Sci. 2010. 5: 550-558.
  11. Lim MJ, Gu YR, Hong JH. Extraction solvent-dependent antioxidant activities and cancer cell growth inhibitory effects of Scutellaria baicalensis extracts. Korean Journal of Food Preservation. 2019. 26: 566-575. https://doi.org/10.11002/kjfp.2019.26.5.566
  12. Lee S, You Y, Kim K, Park J, Jeong C, Jhon DY, Jun W. Antioxidant activities of native Gwangyang Rubus coreanus Muq. Journal of the Korean Society of Food Science and Nutrition. 2012. 41: 327-332. https://doi.org/10.3746/JKFN.2012.41.3.327
  13. Rahmatullah M, Sadeak SMI, Bachar SC. Brine Shrimp Toxicity Study of different Bangladeshi Medicinal Plants. Advances in Natural and Applied Sciences. 2010. 4: 163-173.
  14. Park JJ, Lee JM, Jun WJ. Radical scavenging and anti-obesity effects of various extracts from turmeric (Curcuma longa L.). Journal of the Korean Society of Food Science and Nutrition. 2013. 42: 1908-1914. https://doi.org/10.3746/JKFN.2013.42.12.1908
  15. Park SN. Antioxidative properties of Baicalein, component from Scuteliaria baicalensis Gerogi and its application of cosmetic (I). Journal of Industrial and Engineering Chemistry. 2003. 14: 657-665.
  16. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999. 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  17. Reza H, Haq VM, Das AK, Rahman S, Jahan R, Rahmatullah M. Aanti-hyperglycemic and antinociceptive activity of methanol leaf and stem extract of Nypa fruticans Wurmb. Pakistan Journal of Pharmaceutical Sciences. 2011. 24: 485-488.
  18. Shin SY, Kang MY, Nam SH. Correlation of Lipid Soluble Compounds of Colored Rices and its Mutagenicity, Antimutagenicity and Antioxidativity. Journal of Applied Biological Chemistry. 2003. 46: 214-219.
  19. Song HS, Kim DP, Jung YH, Lee MK. Antioxidant activities of red Hamcho (Salicornia herbacea L.) against lipid peroxidation and the formation of radicals. The Journal of Nutrition and Health. 2007. 20: 150-157.
  20. Yoon SR, Jeong YJ, Lee GD, Kwon JH. Changes in phenolic compounds properties of Rubi fructus extract depending on extraction conditions. Journal of the Korean Society of Food Science and Nutrition. 2003. 32: 338-345. https://doi.org/10.3746/JKFN.2003.32.3.338