Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Light Stress after Heterotrophic Cultivation Enhances Lutein and Biofuel Production from a Novel Algal Strain Scenedesmus obliquus ABC-009

  • Koh, Hyun Gi (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Jeong, Yong Tae (Department of Animal and Plant Research, Nakdonggang National Institute of Biological Resources (NNIBR)) ;
  • Lee, Bongsoo (Department of Microbial Biotechnology, College of Science and Technology, Mokwon University) ;
  • Chang, Yong Keun (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST))
  • Received : 2021.08.17
  • Accepted : 2021.09.26
  • Published : 2022.03.28
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Abstract

Scenedesmus obliquus ABC-009 is a microalgal strain that accumulates large amounts of lutein, particularly when subjected to growth-limiting conditions. Here, the performance of this strain was evaluated for the simultaneous production of lutein and biofuels under three different modes of cultivation - photoautotrophic mode using BG-11 medium with air or 2% CO2 and heterotrophic mode using YM medium. While it was found that the highest fatty acid methyl ester (FAME) level and lutein content per biomass (%) were achieved in BG-11 medium with CO2 and air, respectively, heterotrophic cultivation resulted in much higher biomass productivity. While the cell concentrations of the cultures grown under BG-11 and CO2 were largely similar to those grown in YM medium, the disparity in the biomass yield was largely attributed to the larger cell volume in heterotrophically cultivated cells. Post-cultivation light treatment was found to further enhance the biomass productivity in all three cases and lutein content in heterotrophic conditions. Consequently, the maximum biomass (757.14 ± 20.20 mg/l/d), FAME (92.78 ± 0.08 mg/l/d), and lutein (1.006 ± 0.23 mg/l/d) productivities were obtained under heterotrophic cultivation. Next, large-scale lutein production using microalgae was demonstrated using a 1-ton open raceway pond cultivation system and a low-cost fertilizer (Eco-Sol). The overall biomass yields were similar in both media, while slightly higher lutein content was obtained using the fertilizer owing to the higher nitrogen content.

Keywords

Acknowledgement

This research was supported by the Advanced Biomass R&D Center (ABC) of the Global Frontier Project, funded by the Ministry of Science and ICT (ABC-2010-0029728), a grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NNIBR202102101), and the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT, Korea government (2021R1F1A105127511).

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