DOI QR코드

DOI QR Code

Antibacterial and Antibiofilm Effect of Cell-Free Supernatant of Lactobacillus brevis KCCM 202399 Isolated from Korean Fermented Food against Streptococcus mutans KCTC 5458

  • Kim, Jong Ha (Department of Food Science and Biotechnology of Animal Resource Konkuk University) ;
  • Jang, Hye Ji (Department of Food Science and Biotechnology of Animal Resource Konkuk University) ;
  • Lee, Na-Kyoung (Department of Food Science and Biotechnology of Animal Resource Konkuk University) ;
  • Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resource Konkuk University)
  • 투고 : 2021.09.24
  • 심사 : 2021.10.15
  • 발행 : 2022.01.28

초록

This study aims to determine the antibiofilm effect of cell-free supernatant (CFS) of Lactobacillus brevis strains against Streptococcus mutans strains. To study the antibiofilm mechanism against S. mutans strains, antibacterial effects, cell surface properties (auto-aggregation and cell surface hydrophobicity), exopolysaccharide (EPS) production, and morphological changes were examined. The antibiofilm effect of L. brevis KCCM 202399 CFS as morphological changes were evaluated by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM), compared with the control treatment. Among the L. brevis strains, L. brevis KCCM 202399 showed the highest antibiofilm effect on S. mutans KCTC 5458. The antibacterial effect of L. brevis KCCM 202399 against S. mutans KCTC 5458 was investigated using the deferred method (16.00 mm). The minimum inhibitory concentration of L. brevis KCCM 202399 against S. mutans KCTC 5458 was 25.00%. Compared with the control treatment, L. brevis KCCM 202399 CFS inhibited the bacterial adhesion of S. mutans KCTC 5458 by decreasing auto-aggregation, cell surface hydrophobicity, and EPS production (45.91%, 40.51%, and 67.44%, respectively). L. brevis KCCM 202399 CFS inhibited and eradicated the S. mutans KCTC 5458 biofilm. Therefore, these results suggest that L. brevis KCCM 202399 CFS may be used to develop oral health in the probiotic industry.

키워드

참고문헌

  1. Miki M, Hitoshi K. 2010. Role of two-component system of Streptococcus mutans in the adaptive response to the oral environment. J. Oral Biosci. 52: 252-259. https://doi.org/10.1016/S1349-0079(10)80029-5
  2. Kim HJ, Lee JH. 2020. Anti-biofilm effect of egg yolk phosvitin by inhibition of biomass production and adherence activity against Streptococcus mutans. Food Sci. Anim. Resour. 40: 1001-1013. https://doi.org/10.5851/kosfa.2020.e71
  3. Salli KM, Forssten SD, Lahtinen SJ. Ouwehand AC. 2016. Influence of sucrose and xylitol on an early Streptococcus mutans biofilm in a dental simulator. Arch. Oral Biol. 70: 39-46. https://doi.org/10.1016/j.archoralbio.2016.05.020
  4. Lim SM, Lee NK, Paik HD. 2020. Antibacterial and anticavity activity of probiotic Lactobacillus plantarum 200661 isolated from fermented foods against Streptococcus mutans. LWT - Food Sci. Technol. 118: 108840. https://doi.org/10.1016/j.lwt.2019.108840
  5. Yimeng C, Huiwei Y, Wujun W, Pengfei P, Yin W, Xinyu W, et al. 2020. Killing Streptococcus mutans in mature bioflm with a combination of antimicrobial and antibioflm peptides. Amino Acids 52: 1-14. https://doi.org/10.1007/s00726-019-02804-4
  6. World Health Organization. 2003. Oral health to use from https://www.who.int/news-room/fact-sheets/detail/oral-health. Accessed Mar. 25, 2020.
  7. Baker JL, Faustoferri RC, Quivey Jr RG. 2017. Acid-adaptive mechanisms of Streptococcus mutans-the more we know, the more we don't. Mol. Oral Microbiol. 32: 107-117. https://doi.org/10.1111/omi.12162
  8. Kim AR, Ahn KB, Yun CH, Park OJ, Perinpanayagam H, Yoo YJ, et al. 2019. Lactobacillus plantarum lipoteichoic acid inhibits oral multispecies biofilm. J. Endod. 45: 310-315. https://doi.org/10.1016/j.joen.2018.12.007
  9. Cai JN, Kim MA, Jung JE, Pandit S, Song KY, Jeon JG. 2015. Effects of combined oleic acid and fluoride at sub-MIC levels on EPS formation and viability of Streptococcus mutans UA159 biofilms. Biofouling 31: 555-563. https://doi.org/10.1080/08927014.2015.1076799
  10. Lee NK, Kim SY, Han KJ, Eom SJ, Paik HD. 2014. Probiotic potential of Lactobacillus strains with anti-allergic effects from kimchi for yogurt starters. LWT - Food Sci. Technol. 58: 130-134. https://doi.org/10.1016/j.lwt.2014.02.028
  11. Tareb R, Bemardeau M, Gueguen M, Vernoux JP. 2013. In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria. especially Campylobacter jejuni. J. Med. Microbiol. 62: 637-649. https://doi.org/10.1099/jmm.0.049965-0
  12. Cruz AGD, Ranadheera CS, Nazzaro F, Mortazavian A. 2021. Probiotics and Prebiotics in Foods, pp. 59-80. In Michel RM, Pedro HFS, Luciana PM, Segio S, Arthur CO, Flavia F. Chapter 4 - Probiotics and Prebiotic in Oral Health. Academic Press, Massachusetts.
  13. Parul C, Renuka D, Anuradha S, Neeru B, & Mahesh SD. 2020. A critical appraisal of the effects of probiotics on oral health. J. Funct. Foods 70: 103985. https://doi.org/10.1016/j.jff.2020.103985
  14. Jang HJ, Lee NK, Paik HD. 2019. Probiotic characterization of Lactobacillus brevis KU15153 showing antimicrobial and antioxidant effect isolated from kimchi. Food Sci. Biotechnol. 28: 1521-1528. https://doi.org/10.1007/s10068-019-00576-x
  15. Sorroche F, Bogino P, Russo DM, Zorreguieta A, Nievas F, Morales GM, et al. 2018. Cell autoaggregation biofilm formation, and plant attachment in a Sinorhizobium meliloti lpsB mutant. Mol. Plant Microbe Interact. 31: 1075-1082. https://doi.org/10.1094/mpmi-01-18-0004-r
  16. Chiba A, Sugimoto S, Sato F, Hori S, Mizunoe Y. 2015. A refined technique for extraction of extracellular matrices from bacterial biofilms and its applicability. Microb. Biotechnol. 8: 392-403. https://doi.org/10.1111/1751-7915.12155
  17. Song YJ, Yu HH, Kim YJ, Lee NK, Paik HD. 2019. Anti-biofilm activity of grapefruit seed extract against Staphylococcus aureus and Escherichia coli. J. Mircobiol. Biotechnol. 29: 1177-1183.
  18. Yu HH, Song YJ, Yu HS, Lee NK, Paik HD. 2020. Investigating the antimicrobial and antibiofilm effects of cinnamaldehyde against Campylobacter spp. using cell surface characteristics. J. Food Sci. 85: 157-164. https://doi.org/10.1111/1750-3841.14989
  19. Badet C, Richard B, Castaing-Debat M, De Flaujac, PM, Dorignac G. 2004. Adaptation of salivary Lactobacillus strains to xylitol. Arch. Oral Biol. 49: 161-164. https://doi.org/10.1016/S0003-9969(03)00200-0
  20. Taku F, Yutaka T, Tomonori H, Shigetada K, Takashi O, Shizuo S, et al. 1998. Molecular analyses of glucosyltransferase genes among strains of Streptococcus mutans. FEMS Microbiol. Lett. 161: 331-336. https://doi.org/10.1016/S0378-1097(98)00091-3
  21. Zhang G, Lu M, Liu R, Tian Y, Vu VH, Li Y, et al. 2020. Inhibition of Streptococcus mutans biofilm formation and virulence by Lactobacillus plantarum K41 isolated from traditional sichuan pickles. Front. Microbiol. 11: 774. https://doi.org/10.3389/fmicb.2020.00774
  22. Zhang Z, Lyu X, Xu Q, Li C, Lu M, Gong T, et al. 2020. Utilization of the extract of Cedrus deodara (Roxb. Ex D.Don) G. Don against the biofilm formation and the expression of virulence genes of cariogenic bacterium Streptococcus mutans. J. Ethnopharmacol. 257: 112856. https://doi.org/10.1016/j.jep.2020.112856
  23. Tahmourespour A, Karsa Kermanshahi R, Salehi R, Nabinezhad AAR. 2008. The relationship between cell surface hydrophobicity and antibiotic resistance of Streptococcal strains isolated from dental plaque and caries. Iran. J. Basic Med. Sci. 10: 251-255.
  24. Fang F, Xu J, Li Q, Xia X, Du G. 2018. Characterization of a Lactobaciilus brevis strain with potential oral probiotic properties. BMC Microbiol. 18: 221. https://doi.org/10.1186/s12866-018-1369-3
  25. Yoo Y, Seo DH, Lee H, Cho ES, Song NE, Nam TG, et al. 2019. Inhibitory effect of Bacillus velezensis on biofilm formation by Streptococcus mutans. J. Biotechnol. 298: 57-63. https://doi.org/10.1016/j.jbiotec.2019.04.009
  26. Sun Y, Jiang W, Zhang M, Zhang L, Shen Y, Huang S, et al. 2021. The inhibitory effects of ficin on Streptococcus mutans biofilm formation. Biomed Res. Int. 2021: 6692328.
  27. Jang HJ, Kim JH, Lee NK, Paik HD. 2021. Inhibitory effects of Lactobacillus brevis KU15153 against Streptococcus mutans KCTC 5316 causing dental caries. Microb. Pathog. 157: 104938. https://doi.org/10.1016/j.micpath.2021.104938
  28. Sambanthamoorthy K, Feng X, Patel R, Patel S, Paranavitana C. 2014. Antimicrobial and antibiofilm potential of biosurfactants isolated from lactobacilli against multi-drug-resistant pathogens. BMC Microbiol. 14: 197. https://doi.org/10.1186/1471-2180-14-197
  29. Jamwal A, Sharma K, Chauhan R, Bansal S, Goel G. 2019. Evaluation of commercial probiotic lactic cultures against biofilm formation by Cronobacter sakazakii. Intest. Res. 17: 192-201. https://doi.org/10.5217/ir.2018.00106
  30. Tahmourespour A, Salehi R, Kermanshahi RK. 2011. Lactobacillus acidophilus-derived biosurfactant effect on GTFB and GTFC expression level in Streptococcus mutans biofilm cells. Braz. J. Microbiol. 42: 330-339. https://doi.org/10.1590/S1517-83822011000100042
  31. Ahn KB, Baik JE, Park OJ, Yun CH, Han SH. 2018. Lactobacillus plantarum lipoteichoic acid inhibits biofilm formation of Streptococcus mutans. PLoS One 13: e0192694. https://doi.org/10.1371/journal.pone.0192694
  32. Lin X, Chen X, Tu W, Wang S, Chen H. 2017. Effect of probiotic Lactobacilli on the growth of Streptococcus mutans and multispecies biofilms isolated from children with active caries. Med. Sci. Monit. 23: 4175-4181. https://doi.org/10.12659/MSM.902237
  33. Jeffrey AB. 2004. Virulence properties of Streptococcus. Front. Biosci. 9: 1264-1277.