International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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The effect of rosehip extract on TNF-α, IL-1β, and IL-8 production in THP-1-derived macrophages infected with Aggregatibacter actinomycetemcomitans

  • Song, Yuri (Department of Oral Microbiology, School of Dentistry, Pusan National University) ;
  • Kim, Si young (Department of Oral Microbiology, School of Dentistry, Pusan National University) ;
  • Chung, Jin (Department of Oral Microbiology, School of Dentistry, Pusan National University)
  • Received : 2022.02.11
  • Accepted : 2022.03.15
  • Published : 2022.03.31
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Abstract

Inflammation is a protective mechanism against pathogens, but if maintained continuously, it destroys tissue structures. Aggregatibacter actinomycetemcomitans is a gram-negative, facultative anaerobic bacterium often found in severe periodontitis. A. actinomycetemcomitans invades epithelial cells and triggers inflammatory response in the immune cells. In this study, we investigated the effect of water-soluble rosehip extract on A. actinomycetemcomitans-induced inflammatory responses. A human monocytic cell line (THP-1) was differentiated to macrophages by phorbol 12-mystristate 13-acetate treatment. The cytotoxic effect of extract was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The effects of extract on bacterial growth were examined by measuring the optical densities using a spectrophotometer. THP-1-derived macrophages were infected A. actinomycetemcomitans after extract treatment, and culture supernatants were analyzed for cytokine production using enzyme-linked immunosorbent assay. Protein expression was measured by western blotting. Extract was not toxic to THP-1-derived macrophages. A. actinomycetemcomitans growth was inhibited by 1% extract. The extract suppressed A. actinomycetemcomitans-induced tumor necrosis factor-α, interleukin (IL)-1β, and IL-8 production. It also decreased mitogen-activated protein kinase (MAP kinase) and nuclear factor-κB (NF-κB) phosphorylation. Moreover, the extract inhibited the expression of inflammasome components, including nucleotide-binding oligomerization domain-like receptor pyrin domain-containing protein 3, Absent in Melanoma 2, and apoptosis associated speck-like protein containing a CARD. And cysteine-aspartic proteases-1 and IL-1β expression were decreased by the extract. In summary, extract suppressed A. actinomycetemcomitans growth and decreased inflammatory cytokine production by inhibiting activation of MAP kinase, NF-κB, and inflammasome signaling. Rosehip extract could be effective in the treatment of periodontal inflammation induced by A. actinomycetemcomitans infection.

Keywords

Acknowledgement

This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST; No. NRF-2017M3A9B6062021).

References

  1. Hajishengallis G. Periodontitis: from microbial immune subversion to systemic inflammation. Nat Rev Immunol 2015;15: 30-44. doi: 10.1038/nri3785.
  2. Barth K, Remick DG, Genco CA. Disruption of immune regulation by microbial pathogens and resulting chronic inflammation. J Cell Physiol 2013;228:1413-22. doi: 10.1002/jcp.24299.
  3. Wang X, Li L, Yang M, Geng Y, Chen H, Xu Y, Sun Y. Prevalence and distribution of Aggregatibacter actinomycetemcomitans and its cdtB gene in subgingival plaque of Chinese periodontitis patients. BMC Oral Health 2014;14:37. doi: 10.1186/1472-6831-14-37.
  4. Haffajee AD, Socransky SS, Patel MR, Song X. Microbial complexes in supragingival plaque. Oral Microbiol Immunol 2008;23:196-205. doi: 10.1111/j.1399-302X.2007.00411.x.
  5. Johansson A. Aggregatibacter actinomycetemcomitans leukotoxin: a powerful tool with capacity to cause imbalance in the host inflammatory response. Toxins (Basel) 2011;3:242-59. doi: 10.3390/toxins3030242.
  6. Gursoy UK. Periodontal bacteria and epithelial cell interactions: role of bacterial proteins. Eur J Dent 2008;2:231-2. doi: 10.1055/s-0039-1697385.
  7. Tribble GD, Lamont RJ. Bacterial invasion of epithelial cells and spreading in periodontal tissue. Periodontol 2000 2010; 52:68-83. doi: 10.1111/j.1600-0757.2009.00323.x.
  8. Cekici A, Kantarci A, Hasturk H, Van Dyke TE. Inflammatory and immune pathways in the pathogenesis of periodontal disease. Periodontol 2000 2014;64:57-80. doi: 10.1111/prd.12002.
  9. Kany S, Vollrath JT, Relja B. Cytokines in inflammatory disease. Int J Mol Sci 2019;20:6008. doi: 10.3390/ijms20236008.
  10. Bonito AJ, Lux L, Lohr KN. Impact of local adjuncts to scaling and root planing in periodontal disease therapy: a systematic review. J Periodontol 2005;76:1227-36. doi: 10.1902/jop.2005.76.8.1227. Erratum in: J Periodontol 2006;77:326-7.
  11. Anand B. Herbal therapy in periodontics: a review. J Res Pharm Sci 2017;3:1-7.
  12. Park S, Park SY, Kim KS, Seo GY, Kang S. Rose hip alleviates pain and disease progression in rats with monoiodoacetate induced osteoarthritis. J Korean Soc Appl Biol Chem 2014;57:143-51. doi: 10.1007/s13765-014-4020-5.
  13. Cohen M. Rosehip - an evidence based herbal medicine for inflammation and arthritis. Aust Fam Physician 2012;41:495-8.
  14. Perez-Chaparro PJ, Goncalves C, Figueiredo LC, Faveri M, Lobao E, Tamashiro N, Duarte P, Feres M. Newly identified pathogens associated with periodontitis: a systematic review. J Dent Res 2014;93:846-58. doi: 10.1177/0022034514542468.
  15. Winther K, Vinther Hansen AS, Campbell-Tofte J. Bioactive ingredients of rose hips (Rosa canina L) with special reference to antioxidative and anti-inflammatory properties: in vitro studies. Botanics 2016;6:11-23. doi: 10.2147/BTAT.S91385.
  16. Bickel M. The role of interleukin-8 in inflammation and mechanisms of regulation. J Periodontol 1993;64(5 Suppl):456-60.
  17. Lee JH, Shin MS, Kim EJ, Ahn YB, Kim HD. The association of dietary vitamin C intake with periodontitis among Korean adults: results from KNHANES IV. PLoS One 2017;12:e0177074. doi: 10.1371/journal.pone.0177074.
  18. Botelho J, Machado V, Proenca L, Delgado AS, Mendes JJ. Vitamin D deficiency and oral health: a comprehensive review. Nutrients 2020;12:1471. doi: 10.3390/nu12051471.
  19. Marmol I, Sanchez-de-Diego C, Jimenez-Moreno N, AncinAzpilicueta C, Rodriguez-Yoldi MJ. Therapeutic applications of rose hips from different rosa species. Int J Mol Sci 2017;18:1137. doi: 10.3390/ijms18061137.
  20. Savjani KT, Gajjar AK, Savjani JK. Drug solubility: importance and enhancement techniques. ISRN Pharm 2012;2012:195727. doi: 10.5402/2012/195727.
  21. Huang YK, Tseng KF, Tsai PH, Wang JS, Lee CY, Shen MY. IL-8 as a potential therapeutic target for periodontitis and its inhibition by caffeic acid phenethyl ester in vitro. Int J Mol Sci 2021;22:3641. doi: 10.3390/ijms22073641.
  22. Hienz SA, Paliwal S, Ivanovski S. Mechanisms of bone resorption in periodontitis. J Immunol Res 2015;2015:615486. doi: 10.1155/2015/615486.
  23. Moens U, Kostenko S, Sveinbjornsson B. The role of mitogen-activated protein kinase-activated protein kinases (MAPKAPKs) in inflammation. Genes (Basel) 2013;4:101-33. doi:10.3390/genes4020101.
  24. Vitiello M, D'Isanto M, Galdiero M, Raieta K, Tortora A, Rotondo P, Peluso L, Galdiero M. Interleukin-8 production by THP-1 cells stimulated by Salmonella enterica serovar Typhimurium porins is mediated by AP-1, NF-kappaB and MAPK pathways. Cytokine 2004;27:15-24. doi: 10.1016/j.cyto.2004.03.010.
  25. Shakibaei M, Allaway D, Nebrich S, Mobasheri A. Botanical extracts from rosehip (Rosa canina), willow bark (Salix alba), and nettle leaf (Urtica dioica) suppress IL-1β-induced NF-κB activation in canine articular chondrocytes. Evid Based Complement Alternat Med 2012;2012:509383. doi: 10.1155/2012/509383.
  26. Gonzalez-Manan D, D'Espessailles A, Dossi CG, San Martin M, Mancilla RA, Tapia GS. Rosa mosqueta oil prevents oxidative stress and inflammation through the upregulation of PPAR-α and NRF2 in C57BL/6J mice fed a high-fat diet. J Nutr 2017;147:579-88. doi: 10.3945/jn.116.243261.
  27. Weber A, Wasiliew P, Kracht M. Interleukin-1 (IL-1) pathway. Sci Signal 2010;3:cm1. doi: 10.1126/scisignal.3105cm1.
  28. Park E, Na HS, Song YR, Shin SY, Kim YM, Chung J. Activation of NLRP3 and AIM2 inflammasomes by Porphyromonas gingivalis infection. Infect Immun 2014;82:112-23. doi:10.1128/IAI.00862-13.
  29. Kim S, Park MH, Song YR, Na HS, Chung J. Aggregatibacter actinomycetemcomitans-induced AIM2 inflammasome activation is suppressed by xylitol in differentiated THP-1 macrophages. J Periodontol 2016;87:e116-26. doi: 10.1902/jop.2016.150477.