Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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First molecular detection of canine herpesvirus 1 (CaHV-1) in the Eastern Brazilian Amazon

  • Received : 2021.07.13
  • Accepted : 2021.11.03
  • Published : 2022.03.31
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Abstract

Background: Canine herpesvirus type 1 (CaHV-1) infects dogs and is associated with neonatal deaths and reproductive, ocular, neurological, and respiratory problems. In Brazil, reports of CaHV-1 have been restricted to the southeast and south regions, particularly in municipalities in the state of Rio Grande do Sul. Objectives: To assess the presence and variability of CaHV-1 in canine populations in the state of Pará, North Brazil. Methods: Biological samples from 159 dogs from 4 municipalities in the State of Pará were evaluated using polymerase chain reaction and phylogenetic analyses, with the target being the viral enzyme, thymidine kinase. Results: CaHV-1 was detected in 13 dogs (8.2%), with 2 animals being from the municipality of Santa Bárbara do Pará, 8 from Algodoal Island, 2 from Salinópolis, and one from Capanema. The study sequences revealed 100% identity among themselves and 64% to 100% identity with the other nucleotide sequences from Australia, Brazil, United Kingdom, and United States, including 100% identity with the 2002 isolate from Australia. The 1996 isolate from France was grouped in a branch that was different from the sequence of this study. Conclusions: This study presents the first molecular detection of CaHV-1 in dogs from the Amazon region in northern Brazil. The nucleotide identity between the strains and cytosine insertion in the sequences isolated in this study suggests at least 2 strains of CaHV-1 circulating in Brazil (Pará and BTU-1).

Keywords

Acknowledgement

The authors would like to thank Dr. Jacqueline Kurissio, Dr. Joao Araujo Junior, and the team from the Veterinarians in the Amazon Project: population control of dogs and cats through the application of the One Health, for contributing through donating samples for the study.

References

  1. Carmichael LE, Strandberg JD, Barnes FD. Identification of a cytopathogenic agent infectious for puppies as a canine herpesvirus. Proc Soc Exp Biol Med. 1965;120(3):644-650. https://doi.org/10.3181/00379727-120-30614
  2. Papageorgiou KV, Suarez NM, Wilkie GS, McDonald M, Graham EM, Davison AJ. Genome sequence of canine herpesvirus. PLoS One. 2016;11(5):e0156015. https://doi.org/10.1371/journal.pone.0156015
  3. International Committee on Taxonomy of Viruses. ICTV [Internet]. [publisher unknown]: ICTV; https://talk.ictvonline.org/. Updated 2020. Accessed 2020 Sep 25.
  4. Sykes J, Greene CE. Infectious Diseases of the Dog and Cat. 4th ed. St. Louis: Elsevier; 2012.
  5. Monteiro FL, Cargnelutti JF, Martins M, Anziliero D, Erhardt MM, Weiblen R, et al. Detection of respiratory viruses in shelter dogs maintained under varying environmental conditions. Braz J Microbiol. 2016;47(4):876-881. https://doi.org/10.1016/j.bjm.2016.07.002
  6. Yapici O, Avci O, Bulut O, Hasircioglu S, Kale M, Simsek A, et al. Detection of canine herpesvirus infection on dogs. Microbiol Res J Int. 2018;24(1):1-6.
  7. Cargnelutti JF, Masuda EK, Neuls MG, Weiblen R, Flores EF. Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil. Pesqui Vet Bras. 2015;35(6):557-561. https://doi.org/10.1590/S0100-736X2015000600012
  8. De Freitas LI, Lanzanova TI. Case report: canine herpesvirus type 1 in dog puppies without defined breed. Braz J Anim Environ Res. 2021;4(1):16-27. https://doi.org/10.34188/bjaerv4n1-002
  9. Miyoshi M, Ishii Y, Takiguchi M, Takada A, Yasuda J, Hashimoto A, et al. Detection of canine herpesvirus DNA in the ganglionic neurons and the lymph node lymphocytes of latently infected dogs. J Vet Med Sci. 1999;61(4):375-379. https://doi.org/10.1292/jvms.61.375
  10. Reading MJ, Field HJ. A serological study of canine herpes virus-1 infection in the English dog population. Arch Virol. 1998;143(8):1477-1488. https://doi.org/10.1007/s007050050391
  11. Reagan KL, Sykes JE. Canine infectious respiratory disease. Vet Clin North Am Small Anim Pract. 2020;50(2):405-418. https://doi.org/10.1016/j.cvsm.2019.10.009
  12. Gongora V, Sandoval N, Manchego A. Serological evidence of the presence of canine herpes virus type 1 in Lima province. Rev Investig Vet Peru. 2007;18(2):154-157.
  13. Lara EGV, Romero LBB, Marin LC, Solis JIA, Gallardo SG, Verde CC, et al. Isolation and identification of canine herpesvirus (CHV-1) in Mexico. Open J Pathol. 2016;6(3):111-121. https://doi.org/10.4236/ojpathology.2016.63014
  14. Oliveira EC, Sonne L, Bezerra PS Junior, Teixeira EM, Dezengrini R, Pavarini SP, et al. Clinic and pathological findings in dogs naturally infected with canine herpesvirus. Pesqui Vet Bras. 2009;29(8):637-642. https://doi.org/10.1590/S0100-736X2009000800007
  15. Augusti LM. Anticorpos contra o herpesvirus canino tipo 1 em caes domiciliados e de abrigos no Rio Grande do Sul [dissertation]. Rio Grande do Sul: Universidade Federal de Santa Maria; 2017, p 35.
  16. Da Silva AP. Herpesvirose e adenovirose canina em infeccoes singulares ou mistas: diagnostico molecular e achados clinico-patologicos [dissertation]. Parana: Universidade Estadual de Londrina; 2015, p 66.
  17. Santos TR. Deteccao molecular do herpesvirus canino tipo-1 em cadelas com historico de desordens reprodutivas no Sudeste do Brasil [dissertation]. Uberlandia: Universidade Federal de Uberlandia; 2014, p 44.
  18. Ayres M, Ayres M Jr., Ayres DL, Santos ADAS. Aplicacoes Estatisticas nas Areas das Ciencias Bio-medicas. Belem: Instituto Mamiraua; 2007.
  19. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: a Laboratory Manual. 2nd ed. Plainview: Cold Spring Harbor Laboratory Press; 1989.
  20. Abrao MG, Billerbeck AE, Nishi MY, Marui S, Mendonca BB. Standardization of DNA extraction with NaCl from oral mucosa cells: application in PROP1 gene study. Arq Bras Endocrinol Metabol. 2005;49(6):978-982. https://doi.org/10.1590/S0004-27302005000600019
  21. Kurissio JK. Isolamento viral e diagnostico molecular de herpevirus canino [dissertation]. Sao Paulo: Universidade Estadual Paulista; 2013, p 93.
  22. Bottinelli M, Rampacci E, Stefanetti V, Marenzoni ML, Malmlov AM, Coletti M, et al. Serological and biomolecular survey on canine herpesvirus-1 infection in a dog breeding kennel. J Vet Med Sci. 2016;78(5):797-802. https://doi.org/10.1292/jvms.15-0543
  23. Schulze C, Baumgartner W. Nested polymerase chain reaction and in situ hybridization for diagnosis of canine herpesvirus infection in puppies. Vet Pathol. 1998;35(3):209-217. https://doi.org/10.1177/030098589803500306
  24. Hall T. BioEdit: an important software for molecular biology. Gerf Bull Biosci. 2011;2(1):60-61.
  25. Reubel GH, Pekin J, Webb-Wagg K, Hardy CM. Nucleotide sequence of glycoprotein genes B, C, D, G, H and I, the thymidine kinase and protein kinase genes and gene homologue UL24 of an Australian isolate of canine herpesvirus. Virus Genes. 2002;25(2):195-200. https://doi.org/10.1023/A:1020118003743
  26. Sarker S, Das S, Helbig K, Peters A, Raidal SR. Genome sequence of an Australian strain of canid alphaherpesvirus 1. Aust Vet J. 2018;96(1-2):24-27. https://doi.org/10.1111/avj.12659
  27. Remond M, Sheldrick P, Lebreton F, Nardeux P, Foulon T. Gene organization in the UL region and inverted repeats of the canine herpesvirus genome. J Gen Virol. 1996;77(Pt 1):37-48. https://doi.org/10.1099/0022-1317-77-1-37
  28. Lewin AC, Coghill LM, Mironovich M, Liu CC, Carter RT, Ledbetter EC. Phylogenomic analysis of global isolates of canid alphaherpesvirus 1. Viruses. 2020;12(12):1421. https://doi.org/10.3390/v12121421
  29. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35(6):1547-1549. https://doi.org/10.1093/molbev/msy096
  30. Nunberg JH, Wright DK, Cole GE, Petrovskis EA, Post LE, Compton T, et al. Identification of the thymidine kinase gene of feline herpesvirus: use of degenerate oligonucleotides in the polymerase chain reaction to isolate herpesvirus gene homologs. J Virol. 1989;63(8):3240-3249. https://doi.org/10.1128/jvi.63.8.3240-3249.1989
  31. Burr PD, Campbell ME, Nicolson L, Onions DE. Detection of canine herpesvirus 1 in a wide range of tissues using the polymerase chain reaction. Vet Microbiol. 1996;53(3-4):227-237. https://doi.org/10.1016/S0378-1135(96)01227-8