Journal of Veterinary Science

  • 제23권2호
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  • Pages.35.1-35.13
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  • 2022
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs

  • Cao, Yaqi (College of Life Science and Technology, Guangxi University) ;
  • Dai, ZiFu (College of Life Science and Technology, Guangxi University) ;
  • Lao, Huizhen (College of Life Science and Technology, Guangxi University) ;
  • Zhao, Huimin (College of Life Science and Technology, Guangxi University)
  • 투고 : 2021.12.22
  • 심사 : 2022.01.27
  • 발행 : 2022.03.31
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초록

Background: The testis has been reported to be a naturally O2-deprived organ, dimethyloxaloylglycine (DMOG) can inhibit hypoxia inducible factor-1alpha (HIF-1α) subject to degradation under normal oxygen condition in cells. Objectives: The objective of this study is to detect the effects of DMOG on the proliferation and differentiation of spermatogonial stem cells (SSCs) in Bama minipigs. Methods: Gradient concentrations of DMOG were added into the culture medium, HIF-1α protein in SSCs was detected by western blot analysis, the relative transcription levels of the SSC-specific genes were analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Six days post-induction, the genes related to spermatogenesis were detected by qRT-PCR, and the DNA content was determined by flow cytometry. Results: Results revealed that the levels of HIF-1α protein increased in SSCs with the DMOG treatment in a dose-dependent manner. The relative transcription levels of SSC-specific genes were significantly upregulated (p < 0.05) by activating HIF-1α expression. The induction results showed that DMOG significantly increased (p < 0.05) the spermatogenesis capability of SSCs, and the populations of haploid cells significantly increased (p < 0.05) in DMOG-treated SSCs when compared to those in DMOG-untreated SSCs. Conclusion: We demonstrate that DMOG can promote the spermatogenesis activity of SSCs.

키워드

과제정보

We thank the students of College of Life Science and Technology in Guangxi University who helped in this research and the support by the Natural Science Foundation of China.

참고문헌

  1. Kastelic JP, Rizzoto G, Thundathil J. Review: Testicular vascular cone development and its association with scrotal thermoregulation, semen quality and sperm production in bulls. Animal. 2018;12(s1):s133-s141. https://doi.org/10.1017/S1751731118001167
  2. Ezashi T, Das P, Roberts RM. Low O2 tensions and the prevention of differentiation of hES cells. Proc Natl Acad Sci U S A. 2005;102(13):4783-4788. https://doi.org/10.1073/pnas.0501283102
  3. Francis KR, Wei L. Human embryonic stem cell neural differentiation and enhanced cell survival promoted by hypoxic preconditioning. Cell Death Dis. 2010;1(2):e22. https://doi.org/10.1038/cddis.2009.22
  4. Lee JW, Bae SH, Jeong JW, Kim SH, Kim KW. Hypoxia-inducible factor (HIF-1)α: its protein stability and biological functions. Exp Mol Med. 2004;36(1):1-12. https://doi.org/10.1038/emm.2004.1
  5. Takahashi N, Davy PM, Gardner LH, Mathews J, Yamazaki Y, Allsopp RC. Hypoxia inducible factor 1 alpha is expressed in germ cells throughout the murine life cycle. PLoS One. 2016;11(5):e0154309. https://doi.org/10.1371/journal.pone.0154309
  6. Tang X, Chang C, Hao M, Chen M, Woodley DT, Schonthal AH, et al. Heat shock protein-90alpha (Hsp90α) stabilizes hypoxia-inducible factor-1α (HIF-1α) in support of spermatogenesis and tumorigenesis. Cancer Gene Ther. 2021;28(9):1058-1070. https://doi.org/10.1038/s41417-021-00316-6
  7. Jaakkola P, Mole DR, Tian YM, Wilson MI, Gielbert J, Gaskell SJ, et al. Targeting of HIF-α to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation. Science. 2001;292(5516):468-472. https://doi.org/10.1126/science.1059796
  8. Park HJ, Lee WY, Park C, Hong K, Song H. CD14 is a unique membrane marker of porcine spermatogonial stem cells, regulating their differentiation. Sci Rep. 2019;9(1):9980. https://doi.org/10.1038/s41598-019-46000-6
  9. Simon MC, Keith B. The role of oxygen availability in embryonic development and stem cell function. Nat Rev Mol Cell Biol. 2008;9(4):285-296. https://doi.org/10.1038/nrm2354
  10. Ding H, Gao YS, Wang Y, Hu C, Sun Y, Zhang C. Dimethyloxaloylglycine increases the bone healing capacity of adipose-derived stem cells by promoting osteogenic differentiation and angiogenic potential. Stem Cells Dev. 2014;23(9):990-1000. https://doi.org/10.1089/scd.2013.0486
  11. Wang J, Xue X, Fan K, Liu Q, Zhang S, Peng M, et al. Moderate hypoxia modulates ABCG2 to promote the proliferation of mouse spermatogonial stem cells by maintaining mild ROS levels. Theriogenology. 2020;145:149-157. https://doi.org/10.1016/j.theriogenology.2019.10.007
  12. Vansandt LM, Livesay JL, Dickson MJ, Li L, Pukazhenthi BS, Keefer CL. Conservation of spermatogonial stem cell marker expression in undifferentiated felid spermatogonia. Theriogenology. 2016;86(4):1022-1035.e3. https://doi.org/10.1016/j.theriogenology.2016.03.031
  13. Sharma A, Shah SM, Tiwari M, Roshan M, Singh MK, Singla SK, et al. Propagation of goat putative spermatogonial stem cells under growth factors defined serum-free culture conditions. Cytotechnology. 2020;72(3):489-497. https://doi.org/10.1007/s10616-020-00386-8
  14. Valli H, Sukhwani M, Dovey SL, Peters KA, Donohue J, Castro CA, et al. Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cells. Fertil Steril. 2014;102(2):566-580.e7. https://doi.org/10.1016/j.fertnstert.2014.04.036
  15. Zhao H, Li T, Yang H, Mehmood MU, Lu Y, Liang X, et al. The effects of growth factors on proliferation of spermatogonial stem cells from Guangxi Bama mini-pig. Reprod Domest Anim. 2019;54(12):1574-1582. https://doi.org/10.1111/rda.13566
  16. Gassei K, Sheng Y, Fayomi A, Mital P, Sukhwani M, Lin CC, et al. DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis. Biol Reprod. 2017;96(3):707-719. https://doi.org/10.1095/biolreprod.116.142828
  17. Zhou Z, Shirakawa T, Ohbo K, Sada A, Wu Q, Hasegawa K, et al. RNA binding protein Nanos2 organizes post-transcriptional buffering system to retain primitive state of mouse spermatogonial stem cells. Dev Cell. 2015;34(1):96-107. https://doi.org/10.1016/j.devcel.2015.05.014
  18. Sun F, Xu Q, Zhao D, Degui Chen C. Id4 marks spermatogonial stem cells in the mouse testis. Sci Rep. 2015;5(1):17594. https://doi.org/10.1038/srep17594
  19. Cai H, Jiang Y, Zhang S, Cai NN, Zhu WQ, Yang R, et al. Culture bovine prospermatogonia with 2i medium. Andrologia. 2021;53(6):e14056.
  20. Niu C, Guo J, Shen X, Ma S, Xia M, Xia J, et al. Meiotic gatekeeper STRA8 regulates cell cycle by interacting with SETD8 during spermatogenesis. J Cell Mol Med. 2020;24(7):4194-4211. https://doi.org/10.1111/jcmm.15080
  21. Alrahel A, Movahedin M, Mazaheri Z, Amidi F. Study of Tnp1, Tekt1, and Plzf genes expression during an in vitro three-dimensional neonatal male mice testis culture. Iran Biomed J. 2018;22(4):258-263. https://doi.org/10.29252/ibj.22.4.258