Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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In vitro Root Induction from Shoot Explants of Pear (Pyrus spp.)

  • Jae-Young, Song (National Agrobiodiversity Center, NAS, RDA) ;
  • Jinjoo, Bae (National Agrobiodiversity Center, NAS, RDA) ;
  • Woohyung, Lee (National Agrobiodiversity Center, NAS, RDA) ;
  • Jung-Ro, Lee (National Agrobiodiversity Center, NAS, RDA) ;
  • Mun-Sup, Yoon (National Agrobiodiversity Center, NAS, RDA)
  • Received : 2022.10.17
  • Accepted : 2022.11.07
  • Published : 2022.12.01
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Abstract

The main objective of this study was to identify the most appropriate condition for root formation of in vitro micropropagated pear (Pyrus spp.) plants. In vitro propagation was induced on Murashige and Skoog (MS) medium with 2.0 mg/L of N6-benzyladenine (BA) and 0.2 mg/L of Indole-3-butyric acid (IBA) medium. The short pre-treatment of explants with a high concentration (1 mg/L) of NAA and IBA (R0 medium) in dark for three days, followed by transfer to five different media (R1 to R5) resulted in good rooting responses in the pear 'Oharabani (P. pyrifolia × P. communis)' genotype. For the rooting experiments, the highest rooting percentage (83.3 ± 8.3%), average root length (3.6 ± 1.9 mm), total root number (31 ± 4.0), and average root number per plant (2.6 ± 2.1) were obtained on half strength (1/2) of MS medium supplemented with 30 g/L sucrose without hormones and activated charcoal (AC) (R1 medium). The highest rooting percentage was obtained at 83.3% from explants on R1 and R3 media. The rooting procedure described in this study resulted in good root formation and significantly shorting the root induction time to within 14 days of culture. Further studies are underway to test the suitability of the protocol developed in this study for other pear genotypes.

Keywords

Acknowledgement

This study was carried out with the support of the "Development and application of cryopreservation protocol for pear and freesia germplasm (Project No.PJ016811)", National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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