한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제50권4호
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  • Pages.574-583
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  • 2022
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Ficus vasculosa Wall. ex Miq. Inhibits the LPS-Induced Inflammation in RAW264.7 Macrophages

  • Ji-Won, Park (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jin-Mi, Park (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Sangmi, Eum (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jung Hee, Kim (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jae Hoon, Oh (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jinseon, Choi (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Tran The, Bach (Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology) ;
  • Nguyen, Van Sinh (Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology) ;
  • Sangho, Choi (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kyung-Seop, Ahn (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jae-Won, Lee (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 투고 : 2022.06.08
  • 심사 : 2022.08.30
  • 발행 : 2022.12.28
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초록

Ficus vasculosa Wall. ex Miq. (FV) has been used as a herbal medicine in Southeast Asia and its antioxidant activity has been shown in previous studies. However, it has not yet been elucidated whether FV exerts anti-inflammatory effects on activated-macrophages. Thus, we aimed to evaluate the ameliorative property of FV methanol extract (FM) on lipopolysaccharide (LPS)-induced inflammatory responses and the underlying molecular mechanisms in RAW264.7 macrophages. The experimental results indicated that FM decreased the production of inflammatory mediators (NO/PGE2) and the mRNA/protein expression of iNOS and COX-2 in LPS-stimulated RAW264.7 cells. FM also reduced the secretion of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α and monocyte chemoattractant protein (MCP)-1 in LPS-stimulated RAW264.7 cells. Results also demonstrated that FM improved inflammatory response in LPS-stimulated A549 airway epithelial cells by inhibiting the production of cytokines, such as IL-1β, IL-6 and TNF-α. In addition, FM suppressed MAPK activation and NF-κB nuclear translocation induced by LPS. FM also upregulated the mRNA/protein expression levels of heme oxygenase-1 and the nuclear translocation of nuclear factor erythroid 2-related factor 2 in RAW264.7 cells. In an experimental animal model of LPS-induced acute lung injury, the increased levels of molecules in bronchoalveolar lavage (BAL) fluid were suppressed by FM administration. Collectively, it was founded that FM has anti-inflammatory properties on activated-macrophages by suppressing inflammatory molecules and regulating the activation of MAPK/NF-κB signaling.

키워드

과제정보

This research was supported by grants from KRIBB (Grant No. KGS123221), the Bio & Medical Technology Development Program of the National Research Foundation (NRF) and the Korean government (MSIT) (Grant. No. NRF-2020R1A2C2101228) of the Republic of Korea and the Vietnam Academy of Science and Technology, Project NVCC09.10/22-22.

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