Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Anti-diabetic effects of common buckwheat and tartary buckwheat in type II diabetes animal model

제2형 당뇨 동물모델에서 일반메밀과 쓴메밀의 항당뇨 효과 비교

  • Kim, Su Jeong (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Sohn, Hwang Bae (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Choi, Ji Myung (Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Kimchi Research Institute, Pusan National University) ;
  • Nam, Jung Hwan (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Jong Nam (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Suh, Jong Taek (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Chang, Dong Chil (Bioenergy Crop Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Yul Ho (Central Area Crop Breeding Division, National Institute of Crop Science, Rural Development Administration)
  • 김수정 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 손황배 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 최지명 (부산대학교 식품영양학과 및 김치연구소) ;
  • 조은주 (부산대학교 식품영양학과 및 김치연구소) ;
  • 남정환 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 이종남 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 서종택 (농촌진흥청 국립식량과학원 고령지농업연구소) ;
  • 장동칠 (바이오에너지 작물연구소) ;
  • 김율호 (농촌진흥청 국립식량과학원 중부작물과)
  • Received : 2021.09.25
  • Accepted : 2021.12.13
  • Published : 2022.02.28
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Abstract

In this study, we evaluated the antioxidant and antidiabetic effects of buckwheat. The diabetic animal models were divided into four groups: normal mice group (NOR), streptozotocin-induced diabetic mice group (STZ), group treated with seeds of common or tartary buckwheat (SCB or STB), and the group treated with whole plants of common or tartary buckwheat (PCB or PTB). Rutin content was 44-48 times higher in STB or PTB than in SCB. Oral glucose tolerance and insulin resistance were significantly reduced by treatment with STB, PCB, and PTB. Treatment with PTB also decreased the serum glucose level significantly and the serum insulin levels slightly compared with the STZ group. These results suggest that rutin content and antioxidant activity are closely related to the antidiabetic effect of the treatment. Our results demonstrate that the seeds of tartary buckwheat and whole plants of either common or tartary buckwheat have antidiabetic effects-attenuating blood glucose in an animal model of type II diabetes.

메밀은 전 세계적으로 곡식, 싹, 차 등 다양한 형태로 이용되고 있으며, 주요 재배종으로는 일반메밀과 쓴메밀이 있다. 본 연구는 일반메밀과 쓴메밀 종실과 전초를 대상으로 항산화 및 항당뇨 활성 효과를 평가하였다. 루틴 함량은 쓴메밀 추출물이 일반메밀 추출물보다 44-48배 이상 높았으며, 퀘세틴은 전초에서만 검출되었다. 플라보노이드 및 폴리페놀 함량도 쓴메밀 추출물이 일반메밀 추출물보다 2.5-4.8배 높았다. STZ 투여로 제2형 당뇨가 유발된 동물모델에 일반메밀과 쓴메밀의 종실과 전초를 급여하여 공복혈당, 경구 내당능, 인슐린 내성 변화, 혈중 포도당 및 인슐린 농도 등을 통해 항당뇨 효과를 확인하였다. 종실의 경우는 일반메밀에 비해 쓴메밀 종실군에서 경구 내당능 효과가 뛰어났으며, 혈청 포도당 농도도 유의적으로 감소하는 것을 확인하였다. 전초의 경우, 일반메밀이나 쓴메밀 급여 실험군에서 모두 당뇨대조군에 비하여 유의적인 항당뇨 효과를 보였으며, 인슐린 저항성 지표인 HOMA-IR과 인슐린 민감성 지표인 QUICKI를 비교해본 결과에서도 종실과 전초 섭취군 모두 인슐린 저항성을 감소시키고, 인슐린 민감성을 증가시키는 것을 확인하였다. 이러한 결과들을 고려하면 루틴 함량 및 항산화활성이 쓴메밀 전초의 경우 항당뇨 효과와 밀접한 관계가 있으며, 루틴 함량과 항산화 활성이 높은 쓴메밀 종실 및 일반메밀과 쓴메밀 전초는 항당뇨 식의약 소재로 활용 가능성이 높은 것으로 판단된다.

Keywords

Acknowledgement

본 논문은 농촌진흥청 작물시험연구사업(연구과제: 루틴 고함유 쓴메밀 우량계통 육성 및 이용성 증진 연구, 세부과제: 쓴메밀 가공적성 평가 및 이용 기술 개발, PJ01189403)에 의해 이루어진 것이며 이에 감사드립니다.

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