Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Crab Water Extrct Ameliorates Reflux Esophagitis in Rats

역류성 식도염에 대한 방해(螃蟹) 열수추출물의 보호효과

  • Nam, Hyeon-Hwa (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Seo, Yun-Soo (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Song, Jun-Ho (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, A Yeong (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Noh, Pureum (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong Cheol (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Ji Hye (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine)
  • 남현화 (한국한의학연구원 한약자원연구센터) ;
  • 서윤수 (한국한의학연구원 한약자원연구센터) ;
  • 송준호 (한국한의학연구원 한약자원연구센터) ;
  • 이아영 (한국한의학연구원 한약자원연구센터) ;
  • 노푸름 (한국한의학연구원 한약자원연구센터) ;
  • 문병철 (한국한의학연구원 한약자원연구센터) ;
  • 이지혜 (한국한의학연구원 한약자원연구센터)
  • Received : 2022.01.25
  • Accepted : 2022.02.08
  • Published : 2022.02.28
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Abstract

Objectives: In this study, we aim to demonstrate an effect of crab water extract (CWE) on reflux esophagitis (RE) using lipopolysaccharide (LPS)-induced Raw 264.7 cell and rat model. Methods: To investigate on LPS-induced Raw 264.7 cell, CWE was co-treated with LPS. CWE suppressed Nitric Oxide (NO) production which increased by LPS treatment. Also, CWE showed no cytotoxicity at the concentrations range from 500 ㎍/㎖ to 2000 ㎍/㎖. Next, to investigate the protective effects of CWE on RE rat model, eighteen rats were divided in to three groups: sham group, reflux esophagitis group, and reflux esophagitis pre-treated with 100 mg/kg CWE 1 h before surgery. RE was induced by a pylorus and forestomach ligation operation and all rats were sacrificed after 4 h 30 min from surgery. Results: In gross examination, the CWE administration attenuated esophageal mucosal injury upon histological evaluation of reflux esophagus of rats. The CWE downregulated the expression levels of proteins related to inflammation, such as COX-2 and TNF-α in the esophagus tissue. In addition, the CWE suppressed the NF-κB and IκB-α activation. Conclusions: Based on these findings, we concluded that CWE could possess protective effect against damage to the esophagus due to reflux esophagitis.

Keywords

Acknowledgement

본 연구는 한국한의학연구원 '지속가능한 한약표준자원 활용 기술' (KSN2012320) 사업 지원으로 수행되었으며, 이에 감사드립니다.

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