The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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Th2 Differentiation Control through Formation of Skin Fat Barrier of Sabaek-san extract

사백산 추출물의 지방장벽 생성을 통한 Th2 분화 조절

  • Ahn, Sang Hyun (Dept. of anatomy, College of Korean Medicine, Semyung University) ;
  • Jeong, A Ram (Dept. of Pediatrics, College of Korean Medicine, Gachon University) ;
  • Kim, Ki Bong (School of Korean Medicine, Pusan National University)
  • 안상현 (세명대학교 한의과대학 해부학교실) ;
  • 정아람 (가천대학교 한의과대학 소아과학교실) ;
  • 김기봉 (부산대학교 한의학전문대학원)
  • Received : 2022.01.13
  • Accepted : 2022.02.21
  • Published : 2022.02.28
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Abstract

Objectives The purpose of this study is to confirm the regulate effect of T helper (Th) 2 differentiation that Sabaek-san extract may produce to improves skin lipid barrier damage. Methods Four-weeks-old NC/Nga mice were divided into four groups: control group (Ctrl, n=10), lipid barrier eliminated group (LBE, n=10), Dexamethasone treatment after lipid barrier elimination group (DXT, n=10), and Sabaek-san extract treatment group after lipid barrier elimination (SBT, n=10). Sabaek-san extract was administered for 3 d after removal of the skin fat barrier in SBT group. Then, we identified changes in external symptoms of the skin, factors affecting skin barrier such as potential of hydrogen (pH), filaggrin, transepidermal water loss (TEWL) and Th2 differentiation factors like Interleukin (IL)-4, Kallikrein Related Peptidase 7 (KLK7), and protease activated receptor 2 (PAR-2) through our immunohistochemistry. Results After lipid barrier elimination, the reduction of morphological skin inflammations was less in SBT group than in LBE and DXT group. Also, pH and TEWL were significantly decreased with SBT group. However, filaggrin was significantly increased in SBT group compared to LBE, DXT, and Ctrl group. All kinds of Th2 differentiation factors (IL-4, KLK7 and PAR-2) were also decreased in SBT compared to the LBE and DXT. Conclusions As a result of this study, SBT administration decreased pH, TEWL, and increased filaggrin, thus participating in recovering damaged skin barrier. Since Th2 differentiation factors were decreased as well, SBT's regulatory effect in sequential immune reactions may be a possible explanation of how it enhances recovery of the damaged lipid barrier.

Keywords

Acknowledgement

이 연구는 2019년도 정부의 재원으로 한국연구재단의 지원을 받아 수행된 기초연구사업임 (No. NRF-2019R1A2C1002443).

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