한국응용곤충학회지 (Korean journal of applied entomology)

  • 제61권3호
  • /
  • Pages.449-460
  • /
  • 2022
  • /
  • 1225-0171(pISSN)
  • /
  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
권호 표지
DOI QR코드

DOI QR Code

한국에서 분리된 파밤나방 핵다각체병 바이러스의 전체 유전체 분석

Complete Genome Analysis of Spodoptera exigua Nucleopolyhedrovirus Isolated in Korea

  • 최재방 ((주)옵티팜) ;
  • 김현수 (충북대학교 농업생명환경대학 식물의학과) ;
  • 우수동 (충북대학교 농업생명환경대학 식물의학과)
  • Jae Bang, Choi (Optipharm Inc.) ;
  • Hyun-Soo, Kim (Department of Plant Medicine, College of Agriculture, Life & Environment Science, Chungbuk National University) ;
  • Soo-Dong, Woo (Department of Plant Medicine, College of Agriculture, Life & Environment Science, Chungbuk National University)
  • 투고 : 2022.05.16
  • 심사 : 2022.07.01
  • 발행 : 2022.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

광식성 난방제 해충인 파밤나방(Spodoptera exigua)의 친환경적 방제원으로써 이용을 위해 국내에서 분리된 파밤나방 핵다각체병바이러스(S. exigua nucleopolyhedrovirus K1: SeNPV-K1)의 형태 및 전체 유전체 서열을 분석하였다. SeNPV-K1의 다각체(polyhedra)는 0.6-1.8 um 크기의 부정형으로, 기 보고된 SeNPV와 외형적 차이는 보이지 않았다. 전체 유전체의 염기서열을 분석한 결과, 기 보고된 SeNPV와 비교할 때 145 bp 더 많은 135,756 bp로 확인되었으며, G+C 함량은 44% 였고 상동반복영역은 6개로 두 바이러스간에 차이는 없었다. ORF 분석결과, SeNPV-K1은 기 보고된 것과 비교할 때 2개 더 적은 137개를 가지며, SeNPV-K1에만 존재하는 ORF는 4개가 확인되었다. 이들 4개의 ORF는 비필수 유전자로 바이러스의 특성에는 큰 영향을 주지 않을 것으로 여겨졌다. 유전체의 vista 분석 결과, SeNPV-K1과 기 보고된 SeNPV의 전체 염기서열 유사도가 매우 높은 것으로 확인되었다. 국내에서 처음으로 분석한 SeNPV-K1의 전체 유전체는 기 보고된 SeNPV와 유사한 것으로 나타났으나 서로 다른 분리주로 국내 고유자원임을 확인하였다.

The morphology and whole genome sequence of Spodoptera exigua nucleopolyhedrovirus K1 (SeNPV-K1) isolated in Korea were analyzed for the use as an eco-friendly control source against S. exigua. The polyhedra of SeNPV-K1 was amorphous with a size of 0.6-1.8 ㎛, and there was no external difference with the previously reported SeNPV. As a result of analyzing the nucleotide sequence of the whole genome, it was composed of 135,756 bp, which is 145 bp more than that of the previously reported SeNPV. The G+CG+C content was 44% and there were 6 homologous repeated sequences, so there was no significant difference from the previous report. As a result of ORF analysis, SeNPV-K1 had 137, two fewer than those previously reported, and 4 ORFs present only in SeNPV-K1 were confirmed. These 4 ORFs are non-essential genes and were not considered to have a significant influence on the characteristics of the SeNPV. The genome vista analysis showed that the overall sequence similarity between SeNPV-K1 and the previously reported SeNPV was very high. The whole genome of SeNPV-K1 analyzed for the first time in Korea was found to be similar to the previously reported SeNPV, but it was confirmed that it was a novel resource in Korea with different isolate.

키워드

과제정보

이 논문은 충북대학교 국립대학육성사업(2021)지원을 받아 작성되었음.

참고문헌

  1. Ayres, M.D., Howard, S.C., Kuzio, J., Lopez-Ferber, M., Possee, R.D., 1994. The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology, 202, 586-605. doi: 10.1006/viro.1994.1380.
  2. Black, B.C., Brennan, L.A., Dierks, P.M., Gard, I.E., 1997. Commercialization of baculoviral insecticides. In: Miller, L.K. (ed.), The baculoviruses. The viruses. Springer, Boston, MA. doi: 10.1007/978-1-4899-1834-5_13.
  3. Choi, J.B., Heo, W.I., Shin, T.Y., Bae, S.M., Kim, W.J., Kim, J.I., Kwon, M., Choi, J.Y., Je, Y.H., Jin, B.R., Woo, S.D., 2013. Complete genomic sequences and comparative analysis of Mamestra brassicae nucleopolyhedrovirus isolated in Korea. Virus Genes. 47, 133-151. doi: 10.1007/s11262-013-0922-2.
  4. Felsenstein, J., 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 39, 783-791. doi: 10.1111/j.1558-5646.1985.tb00420.x.
  5. Friesen, P.D., 1997. Regulation of baculovirus early gene expression. In: Miller, L.K. (ed.), The baculoviruses. The viruses. Springer, Boston, MA. doi: 10.1007/978-1-4899-1834-5_6.
  6. Garavaglia, M.J., Miele, S.A.B., Iserte, J.A., Belaich, M.N., Ghiringhelli, P.D., 2012. The ac53, ac78, ac101, and ac103 genes are newly discovered core genes in the family Baculoviridae. J. Virol. 86, 12069-12079. doi: 10.1128/JVI.01873-12. Matias Javier
  7. Hernandez-Martinez, P., Ferre, J., Escriche, B., 2009. Broad-spectrum cross-resistance in Spodoptera exigua from selection with a marginally toxic Cry protein. Pest. Manag. Sci. 65, 645-650. https://doi.org/10.1002/ps.1725
  8. Ijkel, W.F., van Strien, E.A., Heldens, J.G., Broer, R., Zuidema, D., Goldbach, R.W., Vlak, J.M., 1999. Sequence and organization of the Spodoptera exigua multicapsid nucleopolyhedrovirus genome. J. Gen. Virol. 80, 3289-3304. doi: 10.1099/0022-1317-80-12-3289.
  9. Jehle, J.A., Blissard, G.W., Bonning, B.C., Cory, J.S., Herniou, E.A., Rohrmann, G.F., Vlak, J.M., 2006. On the classification and nomenclature of baculoviruses: a proposal for revision. Arch. Virol. 151, 1257-1266. doi: 10.1007/s00705-006-0763-6.
  10. Jin, B.R., Park, B.S., Je, Y.H., Kang, S.K., 1991. Biochemical characteristics of Spodoptera exigua nuclear polyhedrosis virus. Korean J. Appl. Entomol. 30, 144-149.
  11. Kim, Y., Kim, N., 1997. Cold hardiness in Spodoptera exigua (Noctuidae: Lepidoptera). Environ. Entomol. 26, 1117-1123. doi: 10.1093/ee/26.5.1117.
  12. Landais, I., Vincent, R., Bouton, M., Devauchelle, G., DuonorCerutti, M., Ogliastro, M., 2006. Functional analysis of evolutionary conserved clustering of bZIP binding sites in the baculovirus homologous regions (hrs) suggests a cooperativity between host and viral transcription factors. Virology 344, 421-431. doi: 10.1016/j.virol.2005.08.036.
  13. Miele, S.A.B., Garavaglia, M.J., Belaich, M.N., Ghiringhelli, P.D., 2011. Baculovirus: molecular insights on their diversity and conservation. Int. J. Evol. Biol. 2011:379424. doi: 10.4061/2011/379424.
  14. Moar, W.J., Pusztai-Carey, M., Faassen, M.N., Bosch, D., Frutos, R., Rang, C., Luo, K., Adang. M.J., 1995. Development of Bacillus thuringiensis CryIC resistance by Spodoptera exigua (Hubner)(Lepidoptera: Noctuidae). Appl. Environ. Microbiol. 61, 2086-2092. doi: 10.1128/aem.61.6.2086-2092.1995.
  15. Moscardi, F., 1999. Assessment of the application of baculoviruses for control of Lepidoptera. Annu. Rev. Entomol. 44, 257-289. doi: 10.1146/annurev.ento.44.1.257.
  16. O'Reilly, D.R., Miller, L.K., Luckow, V.A., 1994. The baculovirus expression vectors: a laboratory manual. Oxford University Press, Oxford.
  17. Park, J.D., Goh, H.G., 1992. Control of beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae), using synthetic sex pheromone. I. Control by mass trapping in Allium fistulosum Field. Korean J. Appl. Entomol. 31, 45-49.
  18. Rohrmann, G.F., 1986. Polyhedrin structure. J. Gen. Virol. 67, 1499-1513. doi: 10.1099/0022-1317-67-8-1499.
  19. Saitou, N., Nei, M., 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425. doi: 10.1093/oxfordjournals.molbev.a040454.
  20. Smagghe, G., Pineda, S., Carton, B., Estal, P.D., Budia, F., Vinuela, E., 2003. Toxicity and kinetics of methoxyfenozide in greenhouseselected Spodoptera exigua (Lepidoptera: Noctuidae). Pest. Manag. Sci. 59, 1203-1209. doi: 10.1002/ps.756.
  21. Theilmann, D.A., Stewart, S., 1992. Tandemly repeated sequence at the 3ʹ end of the IE-2 gene of the baculovirus Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus is an enhancer element. Virology 187, 97-106. doi: 10.1016/0042-6822(92)90298-4.
  22. Wang, Y., Choi, J.Y., Roh, J.Y., Liu, Q., Tao, X.Y., Park, J.B., Je, Y.H., 2011. Genomic sequence analysis of granulovirus isolated from the tobacco cutworm, Spodoptera litura. Plos One, 6, e28163. doi: 10.1371/journal.pone.0028163.
  23. Wu, W., Liang, H., Kan, J., Liu, C., Yuan, M., Liang, C., Pang, Y., 2008. Autographa californica multiple nucleopolyhedrovirus 38K is a novel nucleocapsid protein that interacts with VP1054, VP39, VP80, and itself. J. Virol. 82, 12356-12364. doi: 10.1128/JVI.00948-08.
  24. Wu, W., Lin, T., Pan, L., Yu, M., Li, Z., Pang, Y., Yang, K., 2006. Autographa californica multiple nucleopolyhedrovirus nucleocapsid assembly is interrupted upon deletion of the 38K gene. J. Virol. 80, 11475-11485. doi: 10.1128/JVI.01155-06.
  25. Zheng, X., Cheng, W., Wang, X., Lei, C., 2011. Enhancement of supercooling capacity and survival by cold acclimation, rapid cold and heat hardening in Spodoptera exigua. Cryobiology, 63. 164-169. doi: 10.1016/j.cryobiol.2011.07.005.
  26. Zhou, J.B., Li, X.Q., De-Eknamkul, W., Suraporn, S., Xu, J.P., 2012. Identification of a new Bombyx mori nucleopolyhedrovirus and analysis of its bro gene family. Virus Genes, 44, 539-547. doi: 10.1007/s11262-012-0721-1.