한국응용곤충학회지 (Korean journal of applied entomology)

  • 제61권4호
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  • Pages.659-664
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  • 2022
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  • 1225-0171(pISSN)
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  • 2287-545X(eISSN)
한국응용곤충학회 (Korean Society of Applied Entomology)
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동결건조 로열젤리 내 putrescine 함량 분석을 위한 분석법 밸리데이션

Validation of UPLC Analysis Method for Putrescine in Lyophilized Royal Jelly

  • 최홍민 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김세건 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김효영 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 우순옥 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 한상미 (농촌진흥청 국립농업과학원 농업생물부)
  • Hong-Min, Choi (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Se-Gun, Kim (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hyo-Young, Kim (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Soon-Ok, Woo (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Sang-Mi, Han (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
  • 투고 : 2022.10.07
  • 심사 : 2022.11.25
  • 발행 : 2022.12.01
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초록

Putrescine은 일반적으로 미생물의 활동에 의해 발생되며, 신선함의 척도로서 사용된다. 그러나 동결건조된 로열젤리에 대한 putrescine의 분석법은 아직 확립되지 않은 실정이다. 본 연구에서는 C18 컬럼을 이용하여 동결건조 로열젤리 내 putrescine을 분석하기 위한 UPLC 분석법을 확립하고자 하였다. 새롭게 확립된 분석법은 7분 이내에 putrescine을 분석 가능하였으며, 이러한 분석법을 검증하기 위해 특이성, 직선성, 정밀성, 정확성, 정량한계, 정성한계 등을 평가하였다. 본 연구를 통해 동결건조 로열젤리의 신선한 정도를 평가하기 위한 분석법을 제공하였으며, 추후 안전성의 척도에 대한 자료로서 활용 가능할 것으로 기대되어진다.

Putrescine generated by the action of microorganisms in the decay generally used as a measure of freshment in food. However, the analytical method of putrescine in freeze-dried royal jelly has not yet been established. In the present study, the UPLC method for putrescine in lyophilized royal jelly was established using C18 column. The newly established method was able to analyze putrescine accurately within 7 minutes and was validated by analytical parameters such as specificity, linearity, precision, accuracy, limit of detection, and limit of quantification. These results provide for the analytical method to evaluate the level of freshment in freeze-dried royal jelly, which will useful in further studies of safety verification.

키워드

과제정보

본 연구는 농촌진흥청 농업기초기반연구(과제번호: PJ01513502)에 의하여 수행되었습니다.

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