Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Validation of UPLC Analysis Method for Putrescine in Lyophilized Royal Jelly

동결건조 로열젤리 내 putrescine 함량 분석을 위한 분석법 밸리데이션

  • Hong-Min, Choi (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Se-Gun, Kim (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Hyo-Young, Kim (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Soon-Ok, Woo (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration) ;
  • Sang-Mi, Han (Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development Administration)
  • 최홍민 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김세건 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 김효영 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 우순옥 (농촌진흥청 국립농업과학원 농업생물부) ;
  • 한상미 (농촌진흥청 국립농업과학원 농업생물부)
  • Received : 2022.10.07
  • Accepted : 2022.11.25
  • Published : 2022.12.01
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Abstract

Putrescine generated by the action of microorganisms in the decay generally used as a measure of freshment in food. However, the analytical method of putrescine in freeze-dried royal jelly has not yet been established. In the present study, the UPLC method for putrescine in lyophilized royal jelly was established using C18 column. The newly established method was able to analyze putrescine accurately within 7 minutes and was validated by analytical parameters such as specificity, linearity, precision, accuracy, limit of detection, and limit of quantification. These results provide for the analytical method to evaluate the level of freshment in freeze-dried royal jelly, which will useful in further studies of safety verification.

Putrescine은 일반적으로 미생물의 활동에 의해 발생되며, 신선함의 척도로서 사용된다. 그러나 동결건조된 로열젤리에 대한 putrescine의 분석법은 아직 확립되지 않은 실정이다. 본 연구에서는 C18 컬럼을 이용하여 동결건조 로열젤리 내 putrescine을 분석하기 위한 UPLC 분석법을 확립하고자 하였다. 새롭게 확립된 분석법은 7분 이내에 putrescine을 분석 가능하였으며, 이러한 분석법을 검증하기 위해 특이성, 직선성, 정밀성, 정확성, 정량한계, 정성한계 등을 평가하였다. 본 연구를 통해 동결건조 로열젤리의 신선한 정도를 평가하기 위한 분석법을 제공하였으며, 추후 안전성의 척도에 대한 자료로서 활용 가능할 것으로 기대되어진다.

Keywords

Acknowledgement

본 연구는 농촌진흥청 농업기초기반연구(과제번호: PJ01513502)에 의하여 수행되었습니다.

References

  1. Bardocz, S., Grant, G., Brown, D.S., Ralph, A., Pusztai, A., 1993. Polyamines in food-implications for growth and health. J. Nutr. Biochem. 4, 66-71. https://doi.org/10.1016/0955-2863(93)90001-D
  2. Choi, H.M., Kim, H.Y., Kim, S.G., Han, S.M., 2021. Optimization of manufacturing process and storage condition of freeze-dried royal jelly for health functional ingredients. Korean J. Apic. 36, 17-21.
  3. Choi, H.M., Kim, H.Y., Kim, S.G., Han, S.M., 2021. Validation and content analysis of putrescine in the venom of honeybee(Apis mellifera L.). Korean J. Appl. Entomo. 60, 263-268. https://doi.org/10.5656/KSAE.2020.03.1.071
  4. Clark, S., Camargo, C.A. 2007. Epidemiology of anaphylaxis. Immunol. Allergy Clin. North Am. 27, 145-163. https://doi.org/10.1016/j.iac.2007.03.002
  5. de Figueiredo, T.C., de Assis, D.C.S., Menezes, L.D.M., da Silva, G.R., Lanza, I.P., Heneine, L.G.D., de Vasconcelos Cancado, S. 2015. HPLC-UV method validation for the identification and quantification of bioactive amines in commercial eggs. Talanta 142, 240-245. https://doi.org/10.1016/j.talanta.2015.04.056
  6. Harro, J., Oreland, L., 2001. Depression as a spreading adjustment disorder of monoaminergic neurons: a case for primary implication of the locus coeruleus. Brain Research Reviews. 38, 79-128. https://doi.org/10.1016/S0165-0173(01)00082-0
  7. He, X., Li, J., Zhao, W., Liu, R., Zhang, L., Kong, X., 2015. Chemical fingerprint analysis for quality control and identification of Ziyang green tea by HPLC. Food Chem. 171, 405-411. https://doi.org/10.1016/j.foodchem.2014.09.026
  8. Ibarra, A.A.G., Wrobel, K., Escobosa, A.R.C., Elguera, J.C.T., Garay-Sevilla, M.E., Wrobel, K., 2015. Determination of putrescine, cadaverine, spermidine and spermine in different chemical matrices by high performance liquid chromatography-electrospray ionization-ion trap tandem mass spectrometry (HPLC-ESI-ITMS/MS). J. Chromatography B. 1002, 176-184. https://doi.org/10.1016/j.jchromb.2015.08.036
  9. Ishimaru, M., Muto, Y., Nakayama, A., Hatate, H., Tanaka, R., 2019. Determination of biogenic amines in fish meat and fermented foods using column-switching high-performance liquid chromatography with fluorescence detection. Food Anal. Methods 12, 166-175. https://doi.org/10.1007/s12161-018-1349-0
  10. Konakovsky, V., Focke, M., Hoffmann-Sommergruber, K., Schmid, R., Scheiner, O., Moser, P., Jarisch, R., Hemmer, W., 2011. Levels of histamine and other biogenic amines in high-quality red wines. Food Addit. Contam. Part A, Chem. Anal. Control. Expo. Risk Assess. 28, 408-416. https://doi.org/10.1080/19440049.2010.551421
  11. Krell, R., 1996. Value-added products from beekeeping. FAO Agricultural Services Bulletin No. 124, Roma.
  12. Lercker, G., Capella, P., Conte, L. S., Ruini, F., Giordani, G., 1981. Components of royal jelly: I. Identification of the organic acids. Lipids. 16, 912-919. https://doi.org/10.1007/BF02534997
  13. Linares, D.M., Martin, M., Ladero, V., Alvarez, M.A., Fernandez, M., 2011. Biogenic amines in dairy products. Crit. Rev. Food Sci. Nutr. 51, 691-703. https://doi.org/10.1080/10408398.2011.582813
  14. Liu, Y., Han, F., Liu, Y., Wang, W., 2020. Determination of biogenic amines in wine using modified liquid-liquid extraction with high performance liquid chromatography-fluorescence detector. Food Anal. Methods. 13, 911-922. https://doi.org/10.1007/s12161-020-01710-w
  15. Mantoanelli, J.O.F., Goncalves, L.M., Pereira, E.A. 2020. Dansyl chloride as a derivatizing agent for the analysis of biogenic amines by CZE-UV. Chromatographia 83, 767-778. https://doi.org/10.1007/s10337-020-03896-x
  16. MFDS, Food Code, 2015. Test method validation guidelines. Osong.
  17. Munoz-Esparza, N.C., Latorre-Moratalla, M.L., Comas-Baste, O., Toro-Funes, N., Veciana-Nogues, M.T., Vidal-Carou, M.C.. 2019. Polyamines in food. Front. Nutr. 6, 108.
  18. Russell, D.H. 1973. The roles of the polyamines, putrescine, spermidine, and spermine in normal and malignant tissues. Life Sci. 13, 1635-1647. https://doi.org/10.1016/0024-3205(73)90111-2
  19. Shalaby, A.R., 1996. Significance of biogenic amines to food safety and human health. Food Res. Int. 29, 675-690. https://doi.org/10.1016/S0963-9969(96)00066-X
  20. Takao, K., Rickhag, M., Hegardt, C., Oredsson, S., persson, L. 2006. Induction of apoptotic cell death by putrescine. Int. J. Biochem. Cell Biol. 38, 621-628. https://doi.org/10.1016/j.biocel.2005.10.020