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표피포도상구균에 대한 에센셜 오일 10종과 옥사실린의 항균비교

Comparison Of Antibacterial Activity Of 10 Essential Oils And Oxacillin Against Staphylococcus Epidermidis

  • 정지은 (단국대학교 보건복지대학원 임상의과학과) ;
  • 육영삼 (단국대학교 보건복지대학원 임상의과학과) ;
  • 김재경 (단국대학교 보건과학대학 임상병리학과)
  • Jeong, Ji Eun (Department of Clinical Medical Science, Graduate School of Health and Welfare, Dankook University) ;
  • Yuk, Young Sam (Department of Clinical Medical Science, Graduate School of Health and Welfare, Dankook University) ;
  • Kim, Jae Kyung (Department of Biomedical Laboratory Science, Dankook University College of Health Sciences)
  • 투고 : 2021.12.02
  • 심사 : 2022.02.20
  • 발행 : 2022.02.28

초록

다양한 연구가 에센셜 오일과 피부 상재균에 관한 초점을 맞추고 있지만, 에센셜 오일의 항균 특성과 미생물에 대한 효능 비교 연구는 거의 없다. 이 연구에서 우리는 옥사실린을 대조군으로 사용하여 표피포도상구균에 대한 10가지 에센셜 오일의 항균 활성을 테스트했다. 두 가지 다른 농도에서 에센셜 오일과 표피포도상구균을 함유한 용액의 활성을 측정하기 위해 광학 밀도(OD)를 사용했다. 옥사실린에 비해 에센셜오일 3종(팔마로사, 레몬그라스, 멜리사 트루)이 항균활성은 더 높았으나 농도는 항균활성에 미미한 영향을 미치는 것으로 나타났다.

Although various studies have focused on the relationship between essential oils (EOs) and skin flora, there are only few comparative studies on the antibacterial properties of EOs and their efficacy against common microorganisms. In this study, we tested the antibacterial activity of 10 different EOs against Staphylococcus epidermidis(S. epidermidis) with oxacillin as control. Optical density was used to measure the activity of solutions containing the EOs and S. epidermidis at two different concentrations. Three EOs (palmarosa, lemongrass, and Mellissa True) had higher antibacterial activity than oxacillin, but their concentrations had negligible effect on antibacterial activity.

키워드

참고문헌

  1. A. Sandilands, C. Sutherland, A. D. Irvine & W. H. I. McLean. (2009). Filaggrin in the frontline: role in skin barrier function and disease. Journal of Cell Science, 122(9), 1285-1294. DOI : 10.1242/jcs.033969
  2. N. R. Kim, S. W. Lee & M. R. Kim. (2020). Study on the antibacterial activity of propolis on Propionibacterium acnes as an acne-inducing bacteria. Journal of the Korean Society of Food Science and Nutrition, 49(6), 586-591. DOI: 10.3746/jkfn.2020.49.6.586
  3. R. Mustarichie, S. Sulistyaningsih & D. Runadi. (2020). Antibacterial activity test of extracts and fractions of cassava leaves (Manihot esculenta Crantz) against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes causing acne. International Journal of Microbiology. DOI: 10.1155/2020/1975904
  4. W. Levinson (2014). Review of medical microbiology and immunology. McGraw-Hill Education (Online). https://lib.hpu.edu.vn/handle/123456789/32671
  5. NNIS System. (2003). National Nosocomial Infections Surveillance (NNIS) System Report, data summary from January 1992 through June 2003, issued August 2003. American Journal of Infection Control, 31(8), 481-498. DOI : 10.1016/j.ajic.2003.09.002
  6. R. Chovanova, M. Mikulasova & S. Vaverkova. (2013). In vitro antibacterial and antibiotic resistance modifying effect of bioactive plant extracts on methicillin-resistant Staphylococcus epidermidis. International Journal of Microbiology, 2013. DOI : 10.1155/2013/760969
  7. H. Yazgan, Y. Ozogul & E. Kuley. (2019). Antimicrobial influence of nanoemulsified lemon essential oil and pure lemon essential oil on food-borne pathogens and fish spoilage bacteria. International Journal of Food Microbiology, 306. 108266. DOI : 10.1016/j.ijfoodmicro.2019.108266.
  8. M. T. Chomnawang, S. Surassmo, V. S. Nukoolkarn & W. Gritsanapan. (2005). Antimicrobial effects of Thai medicinal plants against acne-inducing bacteria. Journal of Ethnopharmacology, 101(1-3), 330-333. https://doi.org/10.1016/j.jep.2005.04.038
  9. S. Khalilzadeh, T. Eftekhar, R. Rahimi, M. Mehriardestani & M. Tabarrai. (2019). An evidence-based review of medicinal plants used for the treatment of vaginitis by Avicenna in "the Canon of Medicine". Galen Medical Journal, 8, e1270. DOI : 10.31661/gmj.v8i0.1270
  10. R. Ribeiro-Santos et al. (2015). A novel insight on an ancient aromatic plant: The rosemary (Rosmarinus officinalis L.). Trends in Food Science & Technology, 45(2), 355-368. https://doi.org/10.1016/j.tifs.2015.07.015
  11. L. M. de Macedo et al. (2020). Rosemary (Rosmarinus officinalis L., syn Salvia rosmarinus Spenn.) and its topical applications: a review. Plants, 9(5), 651. DOI : 10.3390/plants9050651
  12. S. J. Kim, S. H. Park, S. J. Kim & S. H. Park. (2017). Effects of evening primrose oil and evening primrose-rosemary mixed oil on atopic dermatitis-induced animal model. Asian Journal of Beauty and Cosmetology, 5(4), 399-410. DOI : 10.20402/ajbc.2017.0138
  13. B. M. Andrade et al. (2016). Cymbopogon martinii essential oil: chemical characterization, effects on Propionibacterium acnes and immune response in lymphocytes. Planta Medica, 82(S 01), P185. DOI : 10.1055/s-0036-1596342
  14. E. J. Lee, S. H. Lee & M. H. Lim. (2012). Antimicrobial and antioxidative activities of essential oil-focused on Palmarosa (Cymbopogen martini) and Geranium (Pelargonium graveolens). Journal of the Korean Society of Cosmetology, 18, 136-143.
  15. C. P. Morris & P. J. Simner. (2019). Tailoring antimicrobial susceptibility testing to individual species of coagulase-negative staphylococci: next up, Staphylococcus epidermidis. Journal of Clinical Microbiology, 57(12), e01391-01319. DOI : 10.1128/JCM.01391-19
  16. A. K. Lam, M. A. Hill, E. L. Moen, J. Pusavat, C. L. Wouter & C. V. Rice. (2018). Cationic branched polyethylenimine (BPEI) disables antibiotic resistance in methicillin-resistant Staphylococcus epidermidis (MRSE). ChemMedChem, 13(20), 2240. DOI: 10.1002/cmdc.201800433
  17. S. H. Kim, M. R. Yi, C. S. Kim, J. M. Kim & H. J. Bu. (2018). Anti-inflammatory and antimicrobial effects of Jeju rosemary essential oil against skin flora. Journal of the Korean Applied Science and Technology, 35(3), 744-756. DOI : 10.12925/jkocs.2018.35.3.744
  18. S. Bunrathep, P. Thongphasuk, S. Settharaksa & N. Kamkaen. (2017). Effect of an essential oil blend of citronella, lemongrass, and patchouli on acne-causing bacteria. Songklanakarin Journal of Science and Technology, 42(5).
  19. M. Caceres, W. Hidalgo, E. Stashenko, R. Torres & C. Ortiz. (2020). Essential oils of aromatic plants with antibacterial, anti-biofilm and anti-quorum sensing activities against pathogenic bacteria. Antibiotics, 9(4), 147. DOI: 10.3390/antibiotics9040147
  20. M. H. Lodhia, K. R. Bhatt & V.S. Thaker. (2009). Antibacterial activity of essential oils from palmarosa, evening primrose, lavender and tuberose. Indian Journal of Pharmaceutical Sciences, 71(2), 134. DOI : 10.4103/0250-474X.54278
  21. M. N. Boukhatem, A. Kameli, M. A. Ferhat, F. Saidi & K. Tayebi. (2014). The food preservative potential of essential oils: is lemongrass the answer?. Journal fur Verbraucherschutz und Lebensmittelsicherheit, 9(1), 13-21. DOI: 10.1007/s00003-013-0852-x
  22. P. Lertsatitthanakorn & K. Manwiwattanaku (2019). Evaluation of antibacterial activity of lemongrass oil against oral clinical isolates-an in vitro study. Pharmacognosy Journal, 11(5). DOI : 10.5530/pj.2019.11.161
  23. M. W. Joo & J. Y. Kim. (2012). The hindrance effect of essential oil on the growth of scalp microorganisms-focused on clary sage, eucalyptus, cypress, geranium, lemon grass. Journal of The Korean Society of Cosmetology, 18(2).
  24. P. S. Kwon, D. J. Kim & H. Park. (2017). Improved antibacterial effect of blending essential oils. Korean Journal of Clinical Laboratory Science, 49(3), 256-262. DOI : 10.15324/kjcls.2017.49.3.256
  25. N. Mimica-Dukic, B. Bozin, M. Sokovic & N. Simin. (2004). Antimicrobial and antioxidant activities of Melissa officinalis L. (Lamiaceae) essential oil. Journal of Agricultural and Food Chemistry. 52(9), 2485-2489. DOI : 10.1021/jf030698a
  26. M. Kresken, D. Hafner, F. J. Schmitz & T. A. Wichelhaus. (2004). Prevalence of mupirocin resistance in clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis: results of the antimicrobial resistance surveillance study of the Paul-Ehrlich-Society for Chemotherapy, 2001. International journal of antimicrobial agents, 23(6), 577-581. https://doi.org/10.1016/j.ijantimicag.2003.11.007
  27. Y. H. Shin, H. J. Kim, J. Y. Lee, Y. J. Cho & B.J. An. (2012). Major compound analysis and assessment of natural essential oil on anti-oxidative and anti-microbial effects. Journal of Life Science, 22(10), 1344-1351. DOI : 10.5352/JLS.2012.22.10.1344
  28. R. S. Verma et al. (2018). Chemical composition and antibacterial activity of p-menthane chemotype of Cymbopogon martini (Roxb.) W. Watson (Poaceae) from India. Journal of Essential Oil Research. 30(3), 182-188. DOI : 10.1080/10412905.2018.1429327
  29. B. F. Murbach Teles Andrade et al. (2016). The antibacterial effects of Melaleuca alternifolia, Pelargonium graveolens and Cymbopogon martinii essential oils and major compounds on liquid and vapor phase. Journal of Essential Oil Research, 28(3), 227-233. DOI : 10.1080/10412905.2015.1099571