Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Comparison of Conventional Culture Method, Enzyme Immune Method, and PCR for the Rapid Detection of Salmonella spp. in Pet Food

반려동물 사료의 Salmonella spp. 신속검출을 위한 증균배양법, 면역학적 검출법 및 종 특이 프라이머를 이용한 PCR 방법 비교

  • Yun, Hyejeong (Division of Safety Analysis, National Agricultural Products Quality Management Service) ;
  • Cha, Sun Ho (GenoTech Corporation) ;
  • Lee, Seung-Hwa (Division of Safety Analysis, National Agricultural Products Quality Management Service) ;
  • Jeong, Min-Hee (Division of Safety Analysis, National Agricultural Products Quality Management Service) ;
  • Na, Tae-Woong (Division of Safety Analysis, National Agricultural Products Quality Management Service) ;
  • Kim, Haejin (Division of Safety Analysis, National Agricultural Products Quality Management Service) ;
  • Cho, Hyunjeong (Division of Safety Analysis, National Agricultural Products Quality Management Service) ;
  • Hong, Seong-Hee (Division of Safety Analysis, National Agricultural Products Quality Management Service)
  • 윤혜정 (국립농산물품질관리원 시험연구소 성분검정과) ;
  • 차선호 (제노텍 유전체분석센터) ;
  • 이승화 (국립농산물품질관리원 시험연구소 성분검정과) ;
  • 정민희 (국립농산물품질관리원 시험연구소 성분검정과) ;
  • 나태웅 (국립농산물품질관리원 시험연구소 성분검정과) ;
  • 김혜진 (국립농산물품질관리원 시험연구소 성분검정과) ;
  • 조현정 (국립농산물품질관리원 시험연구소 성분검정과) ;
  • 홍성희 (국립농산물품질관리원 시험연구소 성분검정과)
  • Received : 2021.10.14
  • Accepted : 2022.02.21
  • Published : 2022.02.28
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Abstract

The purpose of this study was to compare the conventional culture method, enzyme immune method and the PCR method using species-specific primer in the analysis on the Salmonella spp. found in domestically distributed pet foods. For the study, Salmonella spp. were detected from 175 samples. From the conventional culture method and the PCR method, two samples (jerky and corn gluten) were determined as positive. Also, from the enzyme immune method, one sample (corn gluten) was test-positive. The study revealed that application of the PCR method with species-specific primer allows better distinguishment between the species of the strain collected from the samples than the conventional culture method and/or the enzyme immune method.

국내 유통되는 반려동물 사료의 살모넬라 분석시 증균배양법, 효소면역기법에 의한 분석, 종 특이 primer를 활용한 PCR 방법을 활용하여 비교 평가하였다. 시료 175점 Salmonella spp. 검출 결과 증균배양법 및 종 특이 primer를 활용한 PCR 방법에 의한 검출 방법에서 2점의 시료(육포, 옥수수 글루텐)가 양성으로 확인되었고, 효소면역기법에 의한 검출방법에서는 1점의 시료(옥수수 글루텐)가 양성으로 확인되었다. 증균배양법 및 효소면역기법에 의한 검출방법에 비해 종 특이 primer를 활용한 PCR 방법을 적용 할 경우 시료에서 분리된 균주의 종(species) 판별이 가능하였다.

Keywords

Acknowledgement

본 연구는 2020년도 국립농산물품질관리원 자체연구개발비로 수행되었으며 이에 감사드립니다.

References

  1. Gillespie, J.H., Timoney, J.F., Scott, F,W., Barlough, J.E., 1988. Hagan and Bruner's microbiology and infectious diseases of domestic animals. 8th ed, Comstock Pub. Assoc., Ithaca and London, USA, pp. 74-88.
  2. Linton, A.H., 1983. Guidelines on prevention and control of Salmonellosis. WHO Geneva, pp. 10-128
  3. Wang, X., Jothikumar, N., Griffiths, M.W., Enrichment and DNA extraction protocols for the simultaneous detection of Salmonella and Listeria monocytogenes in raw sausage meat with multiplex real-time PCR. J. Food Prot., 67, 189-192 (2004). https://doi.org/10.4315/0362-028X-67.1.189
  4. Bansal, N.S., Gray, V., McDonell, F., Validated PCR assay for the routine detection of Salmonella in food. J. Food Prot., 69, 282-287 (2006). https://doi.org/10.4315/0362-028X-69.2.282
  5. Yang, B., Qu, D., Zhang, X., Shen, J., Cui, S., Shi, Y., Xi, M., Sheng, M., Zhi, S., Meng, J., Prevalence and characterization of Salmonella serovars in retail meats of marketplace in Shaanxi, China. Int. J. Food Micobiol., 141, 63-72 (2010). https://doi.org/10.1016/j.ijfoodmicro.2010.04.015
  6. Calnek, B.W., Barnes, HJ., 1995. Disease of Poultry. 9th ed, Iowa State University Press, Ames, IA, UAS, pp. 81-129
  7. Chansoo, Lee., (2021, March 1). Food Standards and Standards Notice. Retrieved from https://www.mfds.go.kr/brd/m_211/view.do?seq=14667
  8. Cho, A.R., Dong, H.J., Cho, S.B., Rapid and sensitive detection of Salmonella spp. by using a loop-mediated isothermal amplification assay in duck carcass sample. Food Sci. Anim. Resour., 33, 655-663 (2013). https://doi.org/10.5851/kosfa.2013.33.5.655
  9. Bohaychuk, V.M., Gensler, G.E., McFall. M.E., King. R.K., Renter. D.G., A real-time PCR assay for the detection of Salmonella in a wide variety of food and food animal matrics. J. Food Prot., 70, 1080-1087 (2007). https://doi.org/10.4315/0362-028X-70.5.1080
  10. Melissa, O.P., Maria, C., Martha, E.S., Frank, J., Peter, D., Sensitivity, specificity, and predictive values of three Salmonella rapid detection kits using fresh and frozen poultry environmental samples versus those of standard plating. Appl. Environ. Microbiol., 65, 1055-1060 (1999). https://doi.org/10.1128/aem.65.3.1055-1060.1999
  11. Kim, S.H., Lee, Y.S., Joo, I.S., Kwak, H.S., Chung, G.T., Kim, S.H., Rapid detection for Salmonella spp. by ultrafast real-time PCR Assay. J. Food Hyg. Saf., 33, 50-57 (2018). https://doi.org/10.13103/JFHS.2018.33.1.50
  12. Lampel, K.A., Keasler, S.P., Hanes, D.E., Specific detection of Salmonella enterica serotype Enteritidis using the polymerase chain reaction. Epidemiol. Infect., 116, 137-145 (1996). https://doi.org/10.1017/S0950268800052365
  13. Persson, S., Jacobsen, T., Olsen, J.E., Olsen, K.E.P., Hansen, F., A new real-time PCR method for the identification of Salmonella Dublin. J. Appl. Microbiol., 113, 615-621 (2012). https://doi.org/10.1111/j.1365-2672.2012.05378.x
  14. Luk, J.M., Kongmuang, U., Reeves, P.R., Linberg, A.A., Selective amplification of abequose and paratose synthase genes (rfb) by polymerase chain reaction for identification of Salmonella major serogroups (A, B, C2, and D). J. Clin.. Microbiol., 31, 2118-2123 (1993). https://doi.org/10.1128/jcm.31.8.2118-2123.1993
  15. Bang, M.K., Park, S.J., Kim, Y.J., Kim, J.G., Oh, S.W., Rapid detection of Salmonella spp. in fresh-cut cabbage by Real-time PCR. J. Korean Soc.. Food Sci. Nutr., 39, 1522-1527 (2010). https://doi.org/10.3746/jkfn.2010.39.10.1522
  16. Hyeon, J.Y., Hwang, I.G., Kwak, H.S., Park, J.S., Heo, S., Choi, I.S., Park, C.K., Seo, K.H., Evaluation of an automated ELISA (VIDAS(R)) and Real-time PCR by comparing with a conventional culture method for the detection of Salmonella spp. in steamed pork and raw broccoli sprouts. Korean J. Food Sci. Ani. Resour., 29, 506-512 (2009). https://doi.org/10.5851/KOSFA.2009.29.4.506
  17. De Medici, D., Pezzotti, G., Marfoglia, C., Caciolo, D., Foschi, G., Orefice, L., Comparison between ICS-Vidas, MSRV and standard cultural method for Salmonella recovery in poultry meat. Int. J. Food Microbiol. 45, 205-210 (1998). https://doi.org/10.1016/S0168-1605(98)00157-3
  18. Uyttendaele, M., Vanwildmeersch, K.., Debevere, J., Evaluation of real-time PCR as automated ELISA and a conventional culture method using a semi-solid medium for detection of Salmonella. Lett. Appl. Microbiol., 37, 386-391 (2003). https://doi.org/10.1046/j.1472-765X.2003.01415.x