Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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A Study on Inflammation and Itching of Cyperus rotundus Ethyl Acetate Fractions

향부자 에틸아세테이트 분획물의 염증 및 가려움증에 관한 연구

  • 김도규 ((주)제이투케이바이오 기술연구소) ;
  • 이지연 ((주)제이투케이바이오 기술연구소) ;
  • 문소현 ((주)제이투케이바이오 기술연구소) ;
  • 김유경 ((주)제이투케이바이오 기술연구소) ;
  • 김나리 ((주)제이투케이바이오 기술연구소) ;
  • 정아름 ((주)제이투케이바이오 기술연구소) ;
  • 장준환 ((주)제이투케이바이오 기술연구소) ;
  • 이재섭 ((주)제이투케이바이오 기술연구소) ;
  • 한상배 (충북대학교 약학대학) ;
  • 배준태 ((주)제이투케이바이오 기술연구소)
  • Received : 2022.10.14
  • Accepted : 2022.11.15
  • Published : 2022.12.30
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Abstract

In this study, Cyperus rotundus (C. rotundus) was fractionated into ethyl acetate to identify α-cyperone, a representative indicator, and its ethyl acetate fraction were evaluated to confirm the possibilityas a functional cosmetic ingredient. As a result of HPLC analysis, it was confirmed that the content of α-cyperone was 5.243%. In order to verify the inflammatory relief effect of the ethyl acetate fraction from C. rotundus (EAFC) and α-cyperone, it was confirmed that nitric oxide (NO) production inhibitory ability in RAW 264.7 macrophages induced an inflammatory reaction with lipopolysaccharide (LPS). Real-time qPCR analysis confirmed inhibition of mRNA expression level of inflammatory factors, IL-1β, IL-6, TNF-α, COX-2 and iNOS. As a results of conducting a clinical study using a simple cosmetic formulation containing EAFC, it was confirmed that the skin irritation stimulated by sodium lauryl sulfate (SLS) was calming and relieving itching. Through these results, it is believed that the C. rotundus can be used as a natural cosmetic ingredient that has the effect of inhibiting inflammation and relieving itching.

본 연구에서는 향부자를 이용하여 에틸아세테이트로 분획물을 제조하고, 지표물질 α-cyperone의 함량을 확인하였다. 또한, 향부자 에틸아세테이트 분획물(EAFC) 및 α-cyperone의 항염증 및 가려움증 완화 효능평가를 통하여 기능성 소재로서의 가능성을 확인하였다. EAFC 내의 α-cyperone의 함량을 확인하기 위해 HPLC 분석을 실시한 결과, α-cyperone의 함량이 5.243%임을 확인하였다. EAFC 및 지표성분인 α-cyperone의 염증 완화 효과를 검증하기 위해 lipopolysaccharide (LPS)로 염증반응을 유도시킨 RAW 264.7 대식세포에서의 nitric oxide (NO) 생성 저해능을 확인한 결과, 농도의존적으로 NO 생성을 저해하였다. 추가적인 염증염증 유발 인자인 IL-1β, IL-6, TNF-α, COX-2 및 iNOS의 mRNA 발현 역시 농도의존적으로 저해하였다. EAFC를 함유한 간단한 화장품 제형을 이용하여 임상시험을 실시한 결과, sodium lauryl sulfate (SLS)로 유발된 피부 자극이 진정되는 효과 및 가려움증이 완화되는 효과를 확인하였다. 이를 통하여 향부자는 염증 저해 및 가려움증 완화 효과를 가지는 천연 화장품 원료로 사용 가능할 것으로 사료된다.

Keywords

Acknowledgement

본 연구는 중소벤처기업부와 종소기업기술정보진흥원의 "지역특화산업육성+(R&D, S3089401)" 사업의 지원을 받아 수행된 연구결과입니다.

References

  1. K. I. Kim, K. U. Kim, D. H. Shin, and I. J. Lee, Growth characteristics of purple nutsedge (Cyperus rotundus L.) and establishment of its effective control method, Korean J. Weed Sci, 18(2), 136 (1998).
  2. S. Darmanti, S. Santosa, K. Dewl, and L. H. Nugroh o, Allelopathic effect of Cyperus rotundus L. on seed germination and initial growth of Glycin max L. cv. Grobogan, Bioma, 17(2), 61 (2015). https://doi.org/10.14710/bioma.17.2.61-67
  3. O. Abdessatar and H. S. Fethia, Biological behavior of Cyperus rotundus in relation to agro-ecological conditions and imposed human factors, Afr. J. Plant Sci. 3(1), 63 (2009).
  4. D. S. Akin and D. R. Sh aw, Purple nutsedge (Cyperus rotundus) and yellow nutsedge (Cyperus esculentus) control in glyphosate-tolerant soybea (Glycine max). Weed Technology, 15(3), 564 (2001). https://doi.org/10.1614/0890-037X(2001)015[0564:PNCRAY]2.0.CO;2
  5. A. Bish t, G. R. Bish t, M. Singh , R. Gupta, and V. Singh, Chemical composition and antimicrobial activity of essential oil of burs of Cyperus rotundus Linn, J Pharm Biomed Sci, 17(8), 1637 (2018).
  6. W. G. Seo, H. O. Pae, G. S. Oh, K. Y. Chai, T. O. Kwon, Y. G. Yun, N. Y. Kim, and H. T. Ch ung, Inhibitory effects of methanol extract of Cyperus rotundus rhizomes on nitric oxide and superoxide productions by murine macrophage cell line, RAW 264.7 cells, J Ethnopharmacol, 76(1), 59 (2001). https://doi.org/10.1016/S0378-8741(01)00221-5
  7. H. Weenen, M. H. Nikunya, D. H. Bray, L. B. Mwasumbi, L. S. Kinabo, and V. A. E. B. Kilimali, Antimalarial compounds containing an α, β-unsaturated carbonyl moiety from tanzanian medicinal plants, Planta Med, 56(4), 3171 (1990).
  8. E. Belgin, B. Eyup, D. Gulden, A. Ekrem, and D. Alpaslan, Chemical composition and antimicrobial activities of essential oil and ethanol extract of Cyperus fuscus L. burs from Turkey, Trop J Pharm Res, 17(8), 1637 (2018). https://doi.org/10.4314/tjpr.v17i8.24
  9. S. H. Jung, S. J. Kim, B. G. Jun, K. T. Lee, S. P. Hong, M. S. Oh, D. S. Jang, and J. H. Choi, α-Cyperone, isolated from the rhizomes of Cyperus rotundus, inhibits LPS-induced COX-2 expression and PGE2 production throught the negative regulation of NF-κB signaling in RAW 264.7 cells, J Ethnopharmacol, 147(1), 208 (2013). https://doi.org/10.1016/j.jep.2013.02.034
  10. Y. W. Kim, R. J. Zhao, S. J. Park, J. R. Lee, I. J. Cho, C. H. Yang, S. G. Kim, and S. C. Kim, Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-kappaB-dependent iNOS and proinflammatory cytokines production, Br J Pharmacol, 154(1), 165 (2008). https://doi.org/10.1038/bjp.2008.79
  11. J. L. Wallace, Nitric oxide as a regulator of inflammatory processes, Mem Inst Oswaldo Cruz, 100(1), 5 (2005). https://doi.org/10.1590/S0074-02762005000900002
  12. S. T. Yee, Y. R. Jeong, M. H. Ha, S. H. Kim, M. W. Byun, and S. K. Jo, Induction of nitric oxide and TNF-alpha by herbal plant extracts in mouse aacrophages, J. Korean Soc. Food Sci. Nutr, 29(2), 342 (2000).
  13. D. Sarkar, P. Saha, S. Gamre, S. Bhattacharjee, C. Hariharan, S. Ganguly, R. Sen, G. Mandal, S. Chattopadhyay, S. Majumdar, and M. Chatterjee, Anti-inflammatory effect of allylpyrocatechol in LPS-induced macrophages is mediated by suppression of iNOS and COX-2 via the NF-κB pathway, Int Immunopharmacol, 8(9), 1264 (2008). https://doi.org/10.1016/j.intimp.2008.05.003
  14. M. J. Kim, T. K. Jung, and K. S. Yoon, Anti-aging effects of prescription extracts containing Forsythia viridissima L., J. Soc. Cosmet. Sci. Korea, 41(1), 85 (2015). https://doi.org/10.15230/SCSK.2015.41.1.85
  15. J. Tabart, T. Franck, C. Kevers, J. Pincemail, D. Serteyn, J. O. Defraigne, and J. Dommes, Selective inhibition of inducible cyclooxygenase 2 in vivo is antiinflammatory and nonulcerogenic, Proc. Natl. Acad. Sci. USA, 91(8), 3228 (1994). https://doi.org/10.1073/pnas.91.8.3228
  16. C. S. Eun, E. Y. Hwang, S. O. Lee, S. A. Yang, and M. H. Yu, Anti-oxidant and anti-inflammatory activities of barley sprout extract, J Life Sci, 26(5), 537 (2016). https://doi.org/10.5352/JLS.2016.26.5.537
  17. T. Lawrence, D. A. Willoughby, and D. W. Gilroy, Anti-inflammatory lipid mediators and insights into the resolution of inflammation, Nat. Rev. Immunol., 2(10), 787 (2002). https://doi.org/10.1038/nri915
  18. S. N. Bains, P. Nash , and L. Fonacier, Irritant contact dermatitis, Clin Rev Allergy Immunol, 56(1), 99 (2019). https://doi.org/10.1007/s12016-018-8713-0