International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Developmental characteristics and genetic diversity of the two-spotted cricket Gryllus bimaculatus De Geer, 1773 (Orthoptera: Gryllidae) in South Korea

  • Gyu-Dong, Chang (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences) ;
  • Su Hyun, Yum (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences) ;
  • Jeong-Hun, Song (Industrial Insect and Sericulture Division, Department of Agricultural Biology, National Institute of Agricultural Sciences)
  • Received : 2022.11.28
  • Accepted : 2022.12.15
  • Published : 2022.12.30
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Abstract

In this study, we investigated the developmental characteristics and genetic diversity of seven populations of two-spotted crickets (Gryllus bimaculatus De Geer, 1773 (Orthoptera: Gryllidae)) raised in South Korea. Regarding the developmental characteristics of the species, we observed no statistically significant difference in the weight of the nymphs in the six populations we tested. After molting, although weight differences were observed between the populations in each stage of the developmental period, the average weight for each developmental stage was constant. We also analyzed mitochondrial COI gene sequences (DNA barcoding region) of the reared crickets collected from five insect farms and two national insect rearing facilities and the resultant sequences were analyzed together with the 12 sequences from foreign countries specimens obtained from public data. We detected six haplotypes from 111 specimens, indicating a low intraspecific genetic distance (~1.8%). The most dominant haplotype was overwhelmingly haplotype 1, which was found in all South Korean specimens and four specimens from China, Indonesia, and Germany. These findings indicate that the low genetic diversity of South Korean specimens can be explained by the fact that the G. bimaculatus population imported for feed from Japan in the early 2000s became a maternal group that spread throughout cricket farms in South Korea. In order to breed healthy cricket strains, it is necessary to increase genetic diversity by importing them from other countries through appropriate quarantine procedures.

Keywords

Acknowledgement

We are grateful to H.G. Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, Korea) for their valuable assistance in maintaining colonies of two-spotted crickets. This work was carried out with the support of "The Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01355902)" Rural Development Administration, Republic of Korea. Conflicts of Interest: The authors declare no conflict of interest.

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