International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지
DOI QR코드

DOI QR Code

The difference in bone morphogenic protein-2 expression level among Bombyx mori subspecies

  • Ji Hae, Lee (Industrial Insect and Sericulture Division, National Institute of Agricultural Sciences, RDA) ;
  • Ji-Hyeon, Oh (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Dae-Won, Kim (Department of Oral Biochemistry, College of Dentistry, Gangneung-Wonju National University) ;
  • Seong-Gon, Kim (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • HaeYong, Kweon (Industrial Insect and Sericulture Division, National Institute of Agricultural Sciences, RDA)
  • Received : 2022.09.28
  • Accepted : 2022.11.04
  • Published : 2022.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

There are several subspecies of Bombyx mori, whose silk sericin variants differ. Silk sericin can induce bone morphogenic protein-2 (BMP-2) in macrophages, and silk sericin from different species may have different levels of BMP-2 induction ability. In this study, silk sericin from three B. mori subspecies (Baegokjam, Yeonnokjam, and Goldensilk) was prepared. They were administered to RAW264.7 cells and BMP-2 expression level was studied. Bone regeneration was evaluated using a rat calvarial defect model. BMP-2 expression level was the highest in the Baegokjam group. The bone volume in the Baegokjam group was significantly higher than that in the Yeonnokjam group (P = 0.003). In conclusion, sericin from Baegokjam showed higher levels of BMP-2 expression and bone regeneration than those from Yeonnokjam and Goldensilk.

Keywords

Acknowledgement

This study was conducted with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project no. PJ01562601 and PJ01562603)" Rural Development Administration, Republic of Korea.

References

  1. AOAC (1990) Official methods of analyses of association of analytical chemist (15th ed.). Washington DC: AOAC.
  2. Geiger M, Li RH, Friess W (2003) Collagen sponges for bone regeneration with rhBMP-2. Adv Drug Deliv Rev 55(12), 1613-1629. https://doi.org/10.1016/j.addr.2003.08.010
  3. Ji SD, Kim NS, Kweon H, Choi BH, Kim KY, Koh YH (2016) Nutrition composition differences among steamed and freeze-dried mature silkworm larval powders made from 3 Bombyx mori varieties weaving different colored cocoons. Int J Indust Entomol 33(1), 6-14. https://doi.org/10.7852/IJIE.2016.33.1.6
  4. Jo YY, Kim DW, Choi JY, Kim SG (2019) 4-Hexylresorcinol and silk sericin increase the expression of vascular endothelial growth factor via different pathways. Sci Rep 9, 3448. https://doi.org/10.1038/s41598-019-40027-5
  5. Jo YY, Kweon HY, Oh JH (2020) Sericin for tissue engineering. Appl Sci 10(23), 8457.
  6. Jo YY, Kweon H, Kim DW, Baek K, Chae WS, Kang YJ, et al. (2021) Silk sericin application increases bone morphogenic protein-2/4 expression via a toll-like receptor-mediated pathway. Int J Biol Macromol 190, 607-617. https://doi.org/10.1016/j.ijbiomac.2021.09.021
  7. Kang PD, Sung GB, Kim KY, Lee HS, Kim MJ, Ji SD, et al. (2011) Breeding of new silkworm strain yeonnokjam. Int J Indust Entomol 23(1), 175-178.
  8. Kamalathevan P, Ooi PS, Loo YL (2018) Silk-based biomaterials in cutaneous wound healing: A systematic review. Adv Skin Wound Care 31(12), 565-573. https://doi.org/10.1097/01.ASW.0000546233.35130.a9
  9. Kim SG (2020) Immunomodulation for maxillofacial reconstructive surgery. Maxillofac Plast Reconstr Surg 42(1), 5. https://doi.org/10.1186/s40902-020-00249-4
  10. Kim SG, Jo YY, Kweon HY (2020a) Soluble fraction from silk mat induced bone morphogenic protein in RAW264.7 cells. Int J Indust Entomol 41, 51-55.
  11. Kim DW, Jo YY, Kweon HY, Kim SG (2020b) Different level of tumor necrosis factor-a expression after administration of silk sericin fraction in RAW264.7 cells. Int J Indust Entomol 41, 1-5. https://doi.org/10.7852/IJIE.2020.40.3.1
  12. Kim BJ, Kim SK, Lee JH (2021a) Bone regeneration of demineralized dentin matrix with platelet-rich fibrin and recombinant human bone morphogenetic protein-2 on the bone defects in rabbit calvaria. Maxillofac Plast Reconstr Surg 43, 34. https://doi.org/10.1186/s40902-021-00320-8
  13. Kim SG, Kweon H, Jo YY (2021b) Toll-like receptor and silk sericin for tissue engineering. Int J Indust Entomol 42(1), 1-6. https://doi.org/10.7852/IJIE.2021.42.1.1
  14. Kurioka A, Yamazaki M (2002) Purification and identification of flavonoids from the yellow green cocoon shell (Sasamayu) of the silkworm, Bombyx mori. Biosci Biotech Biochem 66(6), 1396-1399. https://doi.org/10.1271/bbb.66.1396
  15. Kweon H, Yeo J, Kim K, Kim Y, Song H, Kim S, et al. (2009) Characteristics of silk sericin extracted from sericinjam. Int J Indust Entomol 18(2), 121-124.
  16. Kweon, HY, Lee KG, Park KY, Kang SW, Seok YS (2012). Degumming characteristics and color stability of GoldenSilk cocoon. Int J Indust Entomol 24(1), 1-5.
  17. Lee DY, Cho JM, Yun SM, Hong KS, Ji SD, Son JG, et al. (2017) Comparison of silkworm powder from 3 Bombyx mori varieties on alcohol metabolism in rats. Int J Indust Entomol 35(1), 22-29. https://doi.org/10.7852/IJIE.2017.35.1.22
  18. Lee SP, Hong KW, Choi SR (1984) Breeding of new spring silkworm variety 'Baegokjam'. Res Rep RDA 26, 58-64.
  19. Schopfer LM, Onder S, Lockridge O (2022) Organophosphorus pesticides promote protein cross-linking. Chem Res Toxicol 35(9), 1570-1578. https://doi.org/10.1021/acs.chemrestox.2c00194
  20. Selvakumar G, Lonchin S (2022) Bioactive functional collagen-oxidized pullulan scaffold loaded with polydatin for treating chronic wounds. Biomater Adv 140, 213078. https://doi.org/10.1016/j.bioadv.2022.213078
  21. Simmonds MC, Brown JV, Heirs MK, Higgins JP, Mannion RJ, Rodgers MA, et al. (2013) Safety and effectiveness of recombinant human bone morphogenetic protein-2 for spinal fusion: a meta-analysis of individual-participant data. Ann Intern Med 158(12), 877-889. https://doi.org/10.7326/0003-4819-158-12-201306180-00005
  22. Vineis C, Cruz Maya I, Mowafi S, Varesano A, Sanchez Ramirez DO, Abou Taleb M, et al. (2021) Synergistic effect of sericin and keratin in gelatin based nanofibers for in vitro applications. Int J Biol Macromol 190, 375-381. https://doi.org/10.1016/j.ijbiomac.2021.09.007
  23. Wei X, Zhou W, Tang Z, Wu H, Liu Y, Dong H, et al. (2022) Magnesium surface-activated 3D printed porous PEEK scaffolds for in vivo osseointegration by promoting angiogenesis and osteogenesis. Bioact Mater 20, 16-28.
  24. Zara JN, Siu RK, Zhang X, Shen J, Ngo R, Lee M, et al. (2011) High doses of bone morphogenetic protein 2 induce structurally abnormal bone and inflammation in vivo. Tissue Eng Part A 17 (9-10), 1389-1399. https://doi.org/10.1089/ten.TEA.2010.0555
  25. Zhao JJ, Liu DC, Yu YH, Tang H (2021) Development of gelatin-silk sericin incorporated with poly(vinyl alcohol) hydrogel-based nanocomposite for articular cartilage defects in rat knee joint repair. J Biomed Nanotechnol 17(2), 242-252. https://doi.org/10.1166/jbn.2021.3024