Nutrition Research and Practice

  • 제16권1호
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  • Pages.33-45
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  • 2022
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  • 1976-1457(pISSN)
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  • 2005-6168(eISSN)
한국영양학회 (The Korean Nutrition Society)
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Effects of taurine and ginseng extracts on energy metabolism during exercise and their anti-fatigue properties in mice

  • Kim, Jisu (Physical Activity & Performance Institute, Konkuk University) ;
  • Beak, Suji (Research and Development Center, UMUST R&D Corporation) ;
  • Ahn, Sanghyun (Department of Anatomy, College of Korean Medicine, Semyung University) ;
  • Moon, Byung Seok (Department of Nuclear Medicine, Ewha Womans University Seoul Hospital, Ewha Womans University College of Medicine) ;
  • Kim, Bom Sahn (Department of Nuclear Medicine, Ewha Womans University Seoul Hospital, Ewha Womans University College of Medicine) ;
  • Lee, Sang Ju (Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Oh, Seung Jun (Department of Nuclear Medicine, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Park, Hun-Young (Physical Activity & Performance Institute, Konkuk University) ;
  • Kwon, Seung Hae (Seoul Center, Korea Basic Science Institute) ;
  • Shin, Chul Ho (Department of Sports Healthcare management, Namseoul University) ;
  • Lim, Kiwon (Physical Activity & Performance Institute, Konkuk University) ;
  • Lee, Kang Pa (Research and Development Center, UMUST R&D Corporation)
  • 투고 : 2021.10.21
  • 심사 : 2021.06.02
  • 발행 : 2022.02.01
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초록

BACKGROUND/OBJECTIVES: Ginseng extract (GSE) and taurine (TR) are widely used antifatigue resources in functional foods. However, the mechanism underlying the antifatigue effects of GSE and TR are still unclear. Hence, we investigated whether GSE and TR have synergistic effects against fatigue in mice. MATERIALS/METHODS: L6 cells were treated with different concentrations of TR and GSE, and cell viability was determined using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium. Oxidative stress was analyzed by immunocytochemistry using MitoTrackerTM Red FM and an anti-8-oxoguanine antibody. Respiratory gas analysis was performed to investigate metabolism. Expression of an activated protein kinase was analyzed using immunohistochemistry. Gene expression of cluster of differentiation 36 and pyruvate dehydrogenase lipoamide kinase isozyme 4 was measured using reverse transcription-polymerase chain reaction. Mice were orally administered TR, GSE, or their combination for 30 days, and then fatigue-related parameters, including lactate, blood urea nitrogen, and glycogen, were measured after forced swimming. RESULTS: TR and GSE reduced oxidative stress levels in hydrogen peroxide-stimulated L6 cells and enhanced the oxygen uptake and lipid metabolism in mice after acute exercise. After oral administration of TR or GSE for 30 days, the fatigue-related parameters did not change in mice. However, the mice administered GSE (400 mg/kg/day) alone for 30 days could swim longer than those from the other groups. Further, no synergistic effect was observed after the swimming exercise in mice treated with the TR and GSE combination for 30 days. CONCLUSIONS: Taken together, our data suggest that TR and GSE may exert antifatigue effects in mice after acute exercise by enhancing oxygen uptake and lipid oxidation.

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과제정보

This research was supported by the KU Research Professor Program of Konkuk University, the Basic Science Research Program of the National Research Foundation of Korea funded by the Ministry of Science and ICT (2018R1D1A1A02085705), the Korea Health Technology R&D Project of the Korea Health Industry Development Institute funded by the Ministry of Health & Welfare, (HI18C2383) and Korea Basic Science Institute (KBSI) under the R&D programs (D110710) supervised by the Ministry of Science and ICT, Republic of Korea.

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