Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Activity of Anti-Methicillin Resistant Staphylococcus aureus Compound Derived Marine Actinomycetes and Its Synergistic Effect

해양 방선균 유래 항 Methicillin Resistant Staphylococcus aureus 물질의 활성 및 상승 효과

  • 정성윤 (대구가톨릭대학교 바이오메디컬학과)
  • Received : 2022.11.24
  • Accepted : 2022.12.30
  • Published : 2022.12.30
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Abstract

We isolated marine actinomycetes, strain D-5 which produces anti-methicillin resistant Staphylococcus aureus (anti-MRSA) compound. Streptomyces sp. D-5 relatively grew well in the 20~25℃, pH 8.0, and NaCl 3.0%. The ethyl acetate extract of D-5 culture was separated by C18 ODS open column and reverse phase HPLC to yield anti-MRSA compound. The molecular weight of this compound was determined to be 898 by a Liquid chromatograph-mass spectrometer (LC-MS). Compared with penicillin G, this compound showed significant anti-MRSA activity. It also exhibited an inhibition zone of 26 mm at a concentration of 64 ㎍/disk and an inhibition zone of 16 mm at a concentration of 16 ㎍/disk against the MRSA KCCM 40511. Furthermore, the co-treatment of HPLC peak 5 compound and vancomycin caused a more rapid decrease in MRSA cells than each compound alone. It showed 86.8% growth inhibition activity within 12 hours at a low concentration of 50 ㎍/mL during co-treatment, and 97.1% growth in-hibition activity within 48 hours against MRSA KCCM 40511. Taken together, our results suggest that Streptomyces sp. D-5 and its anti-MRSA compound could be employed as a potent agent in MRSA infection.

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Acknowledgement

이 논문은 2020년도 대구가톨릭대학교 학술연구비 지원에 의한 것이며 이에 감사드립니다(20201204).

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