한국어병학회지 (Journal of fish pathology)

  • 제35권2호
  • /
  • Pages.241-246
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  • 2022
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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White spot syndrome virus (WSSV)에 대한 단클론 항체 생산 및 특성 분석

Production and characterization of monoclonal antibodies against white spot syndrome virus (WSSV)

  • 공경희 (전남대학교 수산생명의학과) ;
  • 김춘섭 ((주)엔바이로젠) ;
  • 김도형 (부경대학교 수산생명의학과) ;
  • 김위식 (전남대학교 수산생명의학과)
  • Kyoung-Hui, Kong (Department of Aqualife Medicine, Chonnam National University) ;
  • Choon-Sup, Kim (Enbiogene) ;
  • Kim, Do-Hyung (Department of Aqualife life Medicine, Pukyong National University) ;
  • Wi-Sik, Kim (Department of Aqualife Medicine, Chonnam National University)
  • 투고 : 2022.11.13
  • 심사 : 2022.12.05
  • 발행 : 2022.12.31
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초록

White spot syndrome virus (WSSV) is a prevalent and virulent pathogen affecting cultured whiteleg shrimp (Litopenaeus vannamei) in Korea. In this study, seven monoclonal antibodies (mAbs) (10A12, 16C3, 17G4, 21G5, 22C4, 23B6 and 24G6) were produced by using purified WSSV. The reactivity of these mAbs was analysed by Western blot (WB), indirect immunofluorescence (IIF), and lateral flow immunochromatographic assay (LFIA). WB analysis demonstrated that three mAbs (17G4, 22C4, and 23B6) reacted specifically to VP28 with an approximate molecular weight of 24 kDa, mAb 16C3 reacted with approximately 17 kDa. IIF analysis demonstrated specific fluorescence signals on gill tissues of WSSV-infected shrimp, with five mAbs (10A12, 16C3, 22C4, 23B6, and 24G6), pleopods from WSSV-infected shrimp were used for LFIA, where, two mAbs (21G5 and 22C4) exhibited positive reaction. In conclusion, it can be inferred that the mAbs usage and specificity depends on the nature of assay used for diagnosis.

키워드

과제정보

이 논문은 2019년도 해양수산부 재원으로 해양수산과학기술진흥원의 지원을 받아 수행된 연구임(20190392, 수산생물 검역용 신속 및 정밀진단 기술 개발).

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