Journal of Convergence for Information Technology (융합정보논문지)

Convergence Society for SMB (중소기업융합학회)
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Antioxidant and Anti-inflammatory activity of Colpomenia sinuosa extract

불레기말 추출물의 항산화 및 항염효과

  • Cho, Young Jae (L.FOUNDER INC) ;
  • Kim, Sook-hee (K-Beauty industry fusion, Konkuk Continuing Education Center, Konkuk University) ;
  • Choi, Jae Young (Dept. of Culinary Arts & Hotel Food Service-Major in Culinary Arts, Yeonsung University) ;
  • Lee, Ja-bok (L.FOUNDER INC)
  • 조영재 ((주) 엘파운더) ;
  • 김숙희 (건국대학교 미래지식교육원 학점은행제 K뷰티산업융합학전공) ;
  • 최재영 (연성대학교 호텔외식조리과 호텔조리전공) ;
  • 이자복 ((주) 엘파운더)
  • Received : 2021.10.24
  • Accepted : 2022.01.22
  • Published : 2022.01.28
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Abstract

In this study, the antioxidant and anti-inflammatory properties of Colpomenia sinuosa extracts were identified. Antioxidant experiments included DPPH, ABTS, nitrite scavenging experiments, and FRAP, polyphenol concentration measurements, flavonoid concentration measurements. DPPH assay results showed an antioxidant function of 2.821 mg ascorbic acid/g extract. ABTS assay results showed an antioxidant function of 3.923 mg ascorbic acid/g extract. nitrite assay results showed an antioxidant function of 17.89 mg ascorbic acid/g extract. In FRAP, 1 mg of the Colpomenia sinuosa extract showed a reduction of 2.062±0.177 ㎍ of ascorbic acid. For polyphenols, 62.85±3.18 mg/g was shown. Flavonoids showed 10.01±0.24 mg/g. In the meantime, cell experiments showed cytotoxicity and anti-inflammatory functions. In cytotoxicity experiments, Colpomenia sinuosa extracts showed cytotoxicity of less than 20% and an inflammatory inhibition of 30.93±2.93% at a concentration of 100 ㎍/mL. These results indicate that Colpomenia sinuosa extract is available as functional cosmetic material.

본 연구에서는 불레기말 추출물의 항산화능 및 항염활성을 확인하였다. 라디컬 소거능으로서 DPPH, ABTS, nitrite 소거능 실험, FRAP 실험, 항산화 물질 측정으로서 폴리페놀과 플라보노이드 농도 측정을 실시하였다. DPPH 실험에서는 2.821 mg ascorbic acid / g의 항산화능을 나타내었으며, ABTS 실험에서는 3.923 mg ascorbic acid / g의 항산화능을 나타내었으며, nitrite 소거능 실험에서는 17.89 mg ascorbic acid / g의 항산화능을 나타내었다. FRAP에서는 불레기말 추출물의 1 mg이 ascorbic acid 2.062±0.177 ㎍의 환원력을 보였다. 폴리페놀 농도는 62.85±3.18 mg/g으로 나타났다. 플라보노이드 농도는 10.01±0.24 mg/g으로 나타났다. 한편 세포실험에서는 세포독성과 항염활성을 알아보았다. 세포독성의 경우 20% 이하의 세포 독성을 보였으며, 100 ㎍/mL 농도에서 30.93±2.93%의 염증 억제능을 보여 불레기말 추출물의 기능성 화장품 원료로서의 가능성을 확인하였다.

Keywords

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