The Korean Journal of Pain

The Korean Pain Society (대한통증학회)
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The role of basolateral amygdala orexin 1 receptors on the modulation of pain and psychosocial deficits in nitroglycerin-induced migraine model in adult male rats

  • Askari-Zahabi, Khadijeh (Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman) ;
  • Abbasnejad, Mehdi (Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman) ;
  • Kooshki, Razieh (Department of Biology, Faculty of Sciences, Lorestan University) ;
  • Raoof, Maryam (Department of Orofacial Pain and Dysfunction, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam) ;
  • Esmaeili-Mahani, Saeed (Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman) ;
  • Pourrahimi, Ali Mohammad (Kerman Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences) ;
  • Zamyad, Mahnaz (Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman)
  • Received : 2020.12.21
  • Accepted : 2021.04.23
  • Published : 2022.01.01
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Abstract

Background: Migraine headaches have been associated with sensory hyperactivity and anomalies in social/emotional responses. The main objective of this study was to evaluate the potential involvement of orexin 1 receptors (Orx1R) within the basolateral amygdala (BLA) in the modulation of pain and psychosocial dysfunction in a nitroglycerin (NTG)-induced rat model of migraine. Methods: Adult male Wistar rats were injected with NTG (5 mg/kg, intraperitoneal) every second day over nine days to induce migraine. The experiments were done in the following six groups (6 rats per group): untreated control, NTG, NTG plus vehicle, and NTG groups that were post-treated with intra-BLA microinjection of Orx1R antagonist SB-334867 (10, 20, and 40 nM). Thermal hyperalgesia was assessed using the hot plate and tail-flick tests. Moreover, the elevated plus maze (EPM) and open field (OF) tests were used to assess anxiety-like behaviors. The animals' sociability was evaluated using the three-chamber social task. The NTG-induced photophobia was assessed using a light-dark box. Results: We observed no change in NTG-induced thermal hyperalgesia following administration of SB-334867 (10, 20, and 40 nM). However, SB-334867 (20 and 40 nM) aggravated the NTG-induced anxiogenic responses in both the EPM and OF tasks. The NTG-induced social impairment was overpowered by SB-334867 at all doses. Time spent in the dark chamber of light-dark box was significantly increased in rats treated with SB-334867 (20 and 40 nM/rat). Conclusions: The findings suggest a role for Orx1R within the BLA in control comorbid affective complaints with migraine in rats.

Keywords

Acknowledgement

The authors wish to thank Shahid Bahonar University of Kerman and Kerman University of Medical Sciences for financial support.

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