The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Bacterial Community Structure and Function Shift in Rhizosphere Soil of Tobacco Plants Infected by Meloidogyne incognita

  • Wenjie, Tong (Yunnan Academy of Tobacco Agricultural Sciences) ;
  • Junying, Li (Yunnan Academy of Tobacco Agricultural Sciences) ;
  • Wenfeng, Cong (National Academy of Agriculture Green Development, China Agricultural University) ;
  • Cuiping, Zhang (Yuxi Branch of Yunnan Provincial Tobacco Company) ;
  • Zhaoli, Xu (Yunnan Academy of Tobacco Agricultural Sciences) ;
  • Xiaolong, Chen (Tobacco Leaf Purchase Center, China Tobacco Henan Industrial Co., Ltd.) ;
  • Min, Yang (College of Agronomy, Yunnan Urban Agricultural Engineering & Technological Research Center, Kunming University) ;
  • Jiani, Liu (College of Agronomy, Yunnan Urban Agricultural Engineering & Technological Research Center, Kunming University) ;
  • Lei, Yu (College of Agronomy, Yunnan Urban Agricultural Engineering & Technological Research Center, Kunming University) ;
  • Xiaopeng, Deng (Yunnan Academy of Tobacco Agricultural Sciences)
  • Received : 2022.08.03
  • Accepted : 2022.09.21
  • Published : 2022.12.01
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Abstract

Root-knot nematode disease is a widespread and catastrophic disease of tobacco. However, little is known about the relationship between rhizosphere bacterial community and root-knot nematode disease. This study used 16S rRNA gene sequencing and PICRUSt to assess bacterial community structure and function changes in rhizosphere soil from Meloidogyne incognita-infected tobacco plants. We studied the rhizosphere bacterial community structure of M. incognita-infected and uninfected tobacco plants through a paired comparison design in two regions of tobacco planting area, Yuxi and Jiuxiang of Yunnan Province, southwest China. According to the findings, M. incognita infection can alter the bacterial population in the soil. Uninfested soil has more operational taxonomic unit numbers and richness than infested soil. Principal Coordinate Analysis revealed clear separations between bacterial communities from infested and uninfested soil, indicating that different infection conditions resulted in significantly different bacterial community structures in soils. Firmicutes was prevalent in infested soil, but Chloroflexi and Acidobacteria were prevalent in uninfested soil. Sphingomonas, Streptomyces, and Bradyrhizobium were the dominant bacteria genera, and their abundance were higher in infested soil. By PICRUSt analysis, some metabolism-related functions and signal transduction functions of the rhizosphere bacterial community in the M. incognita infection-tobacco plants had a higher relative abundance than those uninfected. As a result, rhizosphere soils from tobacco plants infected with M. incognita showed considerable bacterial community structure and function alterations.

Keywords

Acknowledgement

This study was supported by the Project of Yunnan Branch Company of China Tobacco Corporation (No. 2019530000241011, 2018530000241016, 2020530000242010).

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