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In Vitro Digestibility and Amino Acid Score of Rhizopus oligosporus Fermented Productsby Domestic Soybean (Glycine max L.) Cultivars

국내산 콩 품종별 Rhizopus oligosporus 발효물의 단백질 소화율과 아미노산가

  • Hye-Young, Park (Dept. of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Hyun-Joo, Kim (Dept. of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Jung Hyun, Seo (Dept. of Southern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Hye Sun, Choi (Dept. of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Jiyoung, Park (Dept. of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Eun-Yeong, Sim (Dept. of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Mi Jung, Kim (Dept. of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Hong-Sig, Kim (Dept. of Central Area Crop Science, National Institute of Crop Science, RDA)
  • 박혜영 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 김현주 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 서정현 (농촌진흥청 국립식량과학원 남부작물부) ;
  • 최혜선 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 박지영 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 심은영 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 김미정 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 김홍식 (농촌진흥청 국립식량과학원 중부작물부)
  • Received : 2022.10.11
  • Accepted : 2022.11.08
  • Published : 2022.12.31

Abstract

In vitro digestibility and protein digestibility corrected amino acid scores (PDCAAS) were investigated to verify the availability of protein in various Rhizopus oligosporus fermented products of domestic soybean (Glycine max L.) cultivars. Danbaegkong (DBK), Daepung (DP), Daewonkong (DWK), Saedanbaek (SDB), Seonyu (SY), and Cheongja4ho (CJ4) were used as raw samples, which were fermented using commercially available Rhizopus oligosporus for 48 h. All cultivars showed increased crude protein content after fermentation. The crude protein content of DBK and SDB was significantly higher than that of the other samples (55.12% in DBK and 54.22% in SDB) (p<0.001). CJ4 had the highest alanine content of 28.88 mg/g (p<0.001), and no significant difference in cysteine content was detected among the cultivars. In most of the fermented samples, the in vitro digestibility was 0.9 or higher, indicating high protein in the fermented samples. However, it is considered that restrictions on digestion are low. In DWK, the amino acid content and PDCAAS, which together indicate protein quality, were 0.917 and 0.855, respectively, confirming that it was the best cultivar to provide the raw material for fermentation. In conclusion Rhizopus oligosporus fermented soybean products can be considered a prospective source of protein with high utility value.

Keywords

Acknowledgement

본 논문은 농촌진흥청 국립식량과학원 연구개발 과제(PJ01608903)의 지원으로 이루어진 것임.

References

  1. Ahmad A, Ramasamy K, Jaafar SM, Majeed ABA, Mani V. 2014. Total isoflavones from soybean and tempeh reversed scopolamine-induced amnesia, improved cholinergic activities and reduced neuroinflammation in brain. Food Chem Toxicol 65:120-128 https://doi.org/10.1016/j.fct.2013.12.025
  2. Angulo-Bejarano PI, Verdugo-Montoya NM, Cuevas-Rodriguez EO, Milan-Carrillo J, Mora-Escobedo R, Lopez-Valenzuela JA, Garzon-Tiznado JA, Reyes-Moreno C. 2008. Tempeh flour from chickpea (Cicer arietinum L.) nutritional and physicochemical properties. Food Chem 106:106-112  https://doi.org/10.1016/j.foodchem.2007.05.049
  3. AOAC International. 2005. Official Method of Analysis AOAC International. 18th ed. Association of Official Analytical Chemists 
  4. Azeke MA, Fretzdorff B, Buening-Pfaue H, Betsche T. 2007. Comparative effect of boiling and solid substrate fer-mentation using the tempeh fungus (Rhizopus oligosporus) on the flatulence potential of African yambean (Sphenostylis stenocarpa L.) seeds. Food Chem 103:1420-1425 https://doi.org/10.1016/j.foodchem.2006.10.058
  5. Committee for the Establishment of a Korean Soybean Museum [CEKSM]. 2005. Soybean. pp.217-220. Korea University Press
  6. Cuevas-Rodriguez EO, Verdugo-Montoya NM, Angulo-Bejarano PI, Milan-Carrillo J, Mora-Escobedo R, Bello-Perez LA, Garzon-Tiznado JA, Reyes-Moreno C. 2006. Nutritional properties of tempeh flour from quality protein maize (Zea mays L.). LWT Food Sci Technol 39:1072-1079 https://doi.org/10.1016/j.lwt.2005.07.003
  7. Egounlety M, Aworh OC. 2003. Effect of soaking, dehulling, cooking and fermentation with Rhizopus oligosporus on the oligosaccharides, trypsin inhibitor, phytic acid and tannins of soybean (Glycine max Merr.), cowpea (Vigna unguiculata L. Walp) and groundbean (Macrotyloma geocarpa Harms). J Food Eng 56:249-254 https://doi.org/10.1016/S0260-8774(02)00262-5
  8. Guebel DV, Nudel BC, Giulietti AM. 1991. A simple and rapid micro-Kjeldahl method for total nitrogen analysis. Biotechnol Tech 5:427-430
  9. Gunawan-Puteri MDPT, Hassanein TR, Prabawati EK, Wijaya CH, Mutukumira AN. 2015. Sensory characteristics of seasoning powders from overripe tempeh, a solid state fermented soybean. Procedia Chem 14:263-269 https://doi.org/10.1016/j.proche.2015.03.037
  10. Ha BK. 2022. Recent soybean industry trends. Soybean Ind Inf 5:4-15
  11. Huang L, Huang Z, Zhang Y, Zhou S, Hu W, Dong M. 2019. Impact of tempeh flour on the rheology of wheat flour dough and bread staling. LWT 111:694-702 https://doi.org/10.1016/j.lwt.2019.04.004
  12. Im JY, Kim SC, Kim S, Choi Y, Yang MR, Cho IH, Kim HR. 2016. Protein and amino-acid contents in Backtae, Seoritae, Huktae, and Seomoktae soybeans with different cooking methods. Korean J Food Cookery Sci 32:567-574 https://doi.org/10.9724/kfcs.2016.32.5.567
  13. Jeong HK, Lee DH, Seo JS, Won SY, Kang HY, Chi JH. 2019. Changes in the characteristics and isoflavone content of soybean on different varieties and processing methods. J Korean Soc Food Sci Nutr 48:245-252 https://doi.org/10.3746/jkfn.2019.48.2.245
  14. Kang YH. 2014. Encyclopedia of Life Science. Available from https://terms.naver.com/entry.naver?docId=436546&cid=60261&categoryId=60261 [cited 4 October 2022]
  15. Kim DM, Jin JS, Kim KH. 1990. Morphological characteristics and proximate compositions of the recommended soybean varieties in Korea. Korean J Food Sci Technol 22:398-404
  16. Kim J. 2017. Anti-obesity effects of black soybean doenjang in C57BL/6 mice. J Life Sci 27:1486-1493 https://doi.org/10.5352/JLS.2017.27.12.1486
  17. Kim JP, Yang YS, Kim JH, Lee HH, Kim ES, Moon YW, Kim JY, Chung JK. 2012. Chemical properties and DPPH radical scavenging ability of sword bean (Canavalia gladiata) extract. Korean J Food Sci Technol 44:441-446 https://doi.org/10.9721/KJFST.2012.44.4.441
  18. Kim MJ, Rhee HS. 1993. Studies on the changes of taste compounds during soy paste fermentaion (III). Korean J Soc Food Sci 9:261-265
  19. Korean Soceity of Food Science and Technology [KoSFoST]. 1998. Encyclopedia of Food Science and Technology. Available from https://terms.naver.com/entry.naver?docId=295047&cid=60262&categoryId=60262 [cited 4 October 2022]
  20. Kurniawati S, Lestiani DD, Damastuti E, Santoso M. 2019. The selenium content of tempeh in Indonesia and its potential contribution to the dietary selenium requirements for adults. J Food Compos Anal 82:103222
  21. Lee CH, Youn Y, Song GS, Kim YS. 2011. Immunostimulatory effects of traditional doenjang. J Korean Soc Food Sci Nutr 40:1227-1234 https://doi.org/10.3746/JKFN.2011.40.9.1227
  22. Lee HC, Park IS. 2006. Fermented Food Science. pp.351-358. Shinkwang
  23. Lee HJ, Moon TH, Noh BS, Chang PS, Baek HH, Lee KK, Kim SJ, Yoo SH, Lee GW. 2014. Food Chemistry. pp.190-193. Soohaksa
  24. Lee IK, Kim JG. 2002. Effects of dietary supplementation of Korean soybean paste (doen-jang) on the lipid metabolism in rats fed a high fat and/or a high cholesterol diet. J Korean Public Health Assoc 28:282-305
  25. Lee SP, Koh KH, Yang JY, Oh SH, Kim JK. 2004. Fermented Food Science. pp. 291-292. Hyoil Munhwasa
  26. Lim HJ, Kim HY, Lee JM, Kim HJ. 2018. The anti-inflammatory influence of fermented soy products containinga fermented Rhus verniciflua extract on lipopolysaccharide (LPS)-treated RAW 264.7 cells. Korean J Food Sci Technol 50:642-652 https://doi.org/10.9721/KJFST.2018.50.6.642
  27. Megazyme. 2019. Mega-CalcTM data calculator protein digestibilityassay (K-PDCAAS)-Instructions. Available from https://view.officeapps.live.com/op/view.aspx?src=https%3A%2F%2Fwww.megazyme.com%2Fdocuments%2FData_Calculator%2FK-PDCAAS_CALC.xlsx&wdOrigin=BROWSELINK [cited 29 October 2022]
  28. Moon HK, Lee SW, Moon JN, Kim DH, Yoon WJ, Kim GY. 2011. Quality characteristics of various beans in distribution. J East Asian Soc Diet Life 21:215-221
  29. Murata K, Ikehata H, Miyamoto T. 1967. Studies on the nutritional value of tempeh. J Food Sci 32:580-586
  30. Park ES, Lee JY, Park KY. 2015. Anticancer effects of black soybean doenjang in HT-29 human colon cancer cells. J Korean Soc Food Sci Nutr 44:1270-1278 https://doi.org/10.3746/jkfn.2015.44.9.1270
  31. Park ES, Yoon S. 1983. The changes of phytic acid content and its interactions with protein and minerals in the preparation of tempeh. Korean J Nutr 16:281-286
  32. Plank DW. 2017. In vitro method for estimating in vivo protein digestibility. US Patent 9,738,920
  33. Rural Development Administration [RDA]. 2021a. Agricultural Technology Guide 116 Soybean. pp.90-101. Rural Development Administration Report No. 11-1390000-004496-14
  34. Rural Development Administration [RDA]. 2021b. Korean Food Composition Table. Available from http://koreanfood.rda.go.kr/kfi/fct/fctFoodSrch/list?menuId=PS03563 [cited 27 September2022]
  35. Schaafsma G. 2005. The protein digestibility-corrected amino acid score (PDCAAS)-A concept for describing protein quality in foods and food ingredients: A critical review. J AOAC Int 88:988-994 https://doi.org/10.1093/jaoac/88.3.988
  36. Shin DS, Choi ID, Lee SK, Park JY, Kim NG, Park CH, Choi HS. 2019. Properties of amino acid and volatile flavor compounds of fermented soybean products by soybean cultivar. Korean J Food Nutr 32:434-441
  37. Soka S, Suwanto A, Rusmana I, Sajuthi D, Iskandriati D, Jessica K. 2015. Analysis of intestinal mucosal immunoglobulin A in sprague dawley rats supplemented with tempeh. HAYATI J Biosci 22:48-52 https://doi.org/10.4308/hjb.22.1.48
  38. Song YE, Han HA, Lee SY, Shin SH, Choi SR, Kim SY. 2019. Quality characteristics and antioxidant activity of regional traditional soybean pastes (deonjang) in Jeonbuk province. Korean J Food Nutr 32:598-610
  39. Stillings BR, Hackler LR. 1965. Amino acid studies on the effect of fermentation time and heat-processing of tempeh. J Food Sci 30:1043-1048 https://doi.org/10.1111/j.1365-2621.1965.tb01884.x
  40. Su HK, Tsai MH, Chao HR, Wu ML, Lu JH. 2021. Data on effect of tempeh fermentation on patients with type II diabetes. Data Brief 38:107310
  41. Wikipedia. 2001. Protein Digestibility Corrected Amino Acid Score. Available from https://en.wikipedia.org/wiki/Protein_Digestibility_Corrected_Amino_Acid_Score [cited 5 October 2022]
  42. Wolkers-Rooijackers JCM, Endika MF, Smid EJ. 2018. Enhancing vitamin B12 in lupin tempeh by in situ fortification. LWT 96:513-518 https://doi.org/10.1016/j.lwt.2018.05.062
  43. Xiao Y, Fan J, Chen Y, Rui X, Zhang Q, Dong M. 2016. Enhanced total phenolic and isoflavone aglycone content, antioxidant activity and DNA damage protection of soybeans processed by solid state fermentation with Rhizopus oligosporus RT-3. RSC Adv 6:29741-29756 https://doi.org/10.1039/c6ra00074f
  44. Yang HJ, Kim MJ, Hong SP. 2019. Anti-diabetic effect of ganjang and doenjang in different aging periods. Korean J Food Preserv 26:300-307 https://doi.org/10.11002/kjfp.2019.26.3.300
  45. Yoon HS, Lee SH, Kang HJ, Eom HJ, Kim Y. 2019. Physicochemical and flavor characteristics of doenjang in Chungbuk provinces during fermentation. Korean J Food Nutr 32:687-695